Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i>
Qualitative and quantitative evaluation of tomatoes carote-noid composition is considered to be the basis of tomato selection. Among known methods of identification and carotenoid content determination thing layer chromatography (TLC) is characterized by inexpensive, quick and availab-le method of a...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"
2018-02-01
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| Series: | Овощи России |
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| Online Access: | https://www.vegetables.su/jour/article/view/461 |
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| author | N. A. Golubkina A. V. Mоlchanova M. M. Tareeva O. G. Baback N. A. Nekrashevich I. Yu. Kondratyeva |
| author_facet | N. A. Golubkina A. V. Mоlchanova M. M. Tareeva O. G. Baback N. A. Nekrashevich I. Yu. Kondratyeva |
| author_sort | N. A. Golubkina |
| collection | DOAJ |
| description | Qualitative and quantitative evaluation of tomatoes carote-noid composition is considered to be the basis of tomato selection. Among known methods of identification and carotenoid content determination thing layer chromatography (TLC) is characterized by inexpensive, quick and availab-le method of analysis. Comparison of individual tomato carotenoid content data obtained using wellknown empirical formulas and based of TLC separation on chromatoraphic paper was achieved. Empirical formulas for the determination of lycopene and beta-carotene concentrations were shown to give high variations in beta-carotene content and decreased values of total carotenoids concentration values. Developed conditions of chromatographic separation and identification of selected carotenoids are based on different polarity of individual pigments and specific absorption spectra of the latter. Method of thin layer chromatography may serve as a quick and effective method for quality evaluation of tomato fruit of different color and determination of beta-carotene, ζ-carotene, neurosporene, lycopene and lutein content. |
| format | Article |
| id | doaj-art-c112cae855a34052850abbd370c998bb |
| institution | Kabale University |
| issn | 2072-9146 2618-7132 |
| language | English |
| publishDate | 2018-02-01 |
| publisher | Federal State Budgetary Scientific Institution "Federal Scientific Vegetable Center" |
| record_format | Article |
| series | Овощи России |
| spelling | doaj-art-c112cae855a34052850abbd370c998bb2025-08-20T03:59:40ZengFederal State Budgetary Scientific Institution "Federal Scientific Vegetable Center"Овощи России2072-91462618-71322018-02-0105969910.18619/2072-9146-2017-5-96-99404Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i>N. A. Golubkina0A. V. Mоlchanova1M. M. Tareeva2O. G. Baback3N. A. Nekrashevich4I. Yu. Kondratyeva5FSBSI, Federal Scientific Vegetable CentreFSBSI, Federal Scientific Vegetable CentreFSBSI, Federal Scientific Vegetable CentreInstitute of genetics and cytologyInstitute of genetics and cytologyFSBSI, Federal Scientific Vegetable CentreQualitative and quantitative evaluation of tomatoes carote-noid composition is considered to be the basis of tomato selection. Among known methods of identification and carotenoid content determination thing layer chromatography (TLC) is characterized by inexpensive, quick and availab-le method of analysis. Comparison of individual tomato carotenoid content data obtained using wellknown empirical formulas and based of TLC separation on chromatoraphic paper was achieved. Empirical formulas for the determination of lycopene and beta-carotene concentrations were shown to give high variations in beta-carotene content and decreased values of total carotenoids concentration values. Developed conditions of chromatographic separation and identification of selected carotenoids are based on different polarity of individual pigments and specific absorption spectra of the latter. Method of thin layer chromatography may serve as a quick and effective method for quality evaluation of tomato fruit of different color and determination of beta-carotene, ζ-carotene, neurosporene, lycopene and lutein content.https://www.vegetables.su/jour/article/view/461carotenoidstomatoesthin layer chromatography |
| spellingShingle | N. A. Golubkina A. V. Mоlchanova M. M. Tareeva O. G. Baback N. A. Nekrashevich I. Yu. Kondratyeva Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> Овощи России carotenoids tomatoes thin layer chromatography |
| title | Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> |
| title_full | Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> |
| title_fullStr | Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> |
| title_full_unstemmed | Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> |
| title_short | Quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes <i>Solanum lycopersicum</i> |
| title_sort | quantitative thing layer chromatography for evaluation of carotenoid composition of tomatoes i solanum lycopersicum i |
| topic | carotenoids tomatoes thin layer chromatography |
| url | https://www.vegetables.su/jour/article/view/461 |
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