EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway
[Objective:] To investigate the effect of early growth response gene 1 (EGR1) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) by regulating long non-coding RNA human leukocyte antigen complex group 11 (lnc-HCG11) and wingless/β-catenin (Wnt/β-catenin) pathway. [Metho...
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Editorial Office of Journal of Oral and Maxillofacial Surgery
2025-04-01
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| Series: | Kouqiang hemian waike zazhi |
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| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.02.005 |
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| author | LI Cheng LONG Xiangfeng ZHOU Qin XU Fangfang HUANG Xuecheng MAO Jiatuan |
| author_facet | LI Cheng LONG Xiangfeng ZHOU Qin XU Fangfang HUANG Xuecheng MAO Jiatuan |
| author_sort | LI Cheng |
| collection | DOAJ |
| description | [Objective:] To investigate the effect of early growth response gene 1 (EGR1) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) by regulating long non-coding RNA human leukocyte antigen complex group 11 (lnc-HCG11) and wingless/β-catenin (Wnt/β-catenin) pathway. [Methods:] The expression levels of EGR1 and lnc-HCG11 were changed in hPDLSCs through gene overexpression and interference assays, while the activation level of the Wnt/β-catenin pathway was altered by the β-catenin inhibitor IWR-1. Subsequently, real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting experiments were conducted to validate the intervention effect. After osteogenic induction, RT-qPCR was used to detect the expression levels of osteogenic genes runt-related transcription factor 2 (RUNX2), collagen type Ⅰα 1 (COL1A1), osteopontin (OPN), osteocalcin (OCN), and alizarin red staining was used to detect the mineralization level of cells. [Results:] The overexpression of EGR1 was observed to promote the expression of lnc-HCG11 in hPDLSCs, while the overexpression of lnc-HCG11 promoted the expression levels of RUNX2, COL1A1, OPN, OCN, and mineralization in hPDLSCs. The pro-osteogenic effect of EGR1 was inhibited by silencing lnc-HCG11, while treatment with IWR-1 suppressed the expression of active β-catenin and weakened the pro-osteogenic effect of lnc-HCG11 on hPDLSCs. [Conclusion:] EGR1 promotes osteogenic differentiation of hPDLSCs by activating the lnc-HCG11/Wnt/β-catenin pathway. |
| format | Article |
| id | doaj-art-c10eee24a5664ac5ae9cc205386de2b1 |
| institution | Kabale University |
| issn | 1005-4979 |
| language | zho |
| publishDate | 2025-04-01 |
| publisher | Editorial Office of Journal of Oral and Maxillofacial Surgery |
| record_format | Article |
| series | Kouqiang hemian waike zazhi |
| spelling | doaj-art-c10eee24a5664ac5ae9cc205386de2b12025-08-25T06:12:07ZzhoEditorial Office of Journal of Oral and Maxillofacial SurgeryKouqiang hemian waike zazhi1005-49792025-04-0135211612210.12439/kqhm.1005-4979.2025.02.005EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathwayLI Cheng0LONG Xiangfeng1ZHOU Qin2XU Fangfang3HUANG Xuecheng4MAO Jiatuan5Department of Stomatology, Jing'an District Institute of Dantal Diseases, Shanghai 200040Department of Stomatology, Guangnan People's Hospital, Wenshan 663300, ChinaDepartment of Stomatology, Jing'an District Institute of Dantal Diseases, Shanghai 200040Department of Stomatology, Jing'an District Institute of Dantal Diseases, Shanghai 200040Department of Stomatology, Guangnan People's Hospital, Wenshan 663300, ChinaDepartment of Stomatology, Guangnan People's Hospital, Wenshan 663300, China[Objective:] To investigate the effect of early growth response gene 1 (EGR1) on osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) by regulating long non-coding RNA human leukocyte antigen complex group 11 (lnc-HCG11) and wingless/β-catenin (Wnt/β-catenin) pathway. [Methods:] The expression levels of EGR1 and lnc-HCG11 were changed in hPDLSCs through gene overexpression and interference assays, while the activation level of the Wnt/β-catenin pathway was altered by the β-catenin inhibitor IWR-1. Subsequently, real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting experiments were conducted to validate the intervention effect. After osteogenic induction, RT-qPCR was used to detect the expression levels of osteogenic genes runt-related transcription factor 2 (RUNX2), collagen type Ⅰα 1 (COL1A1), osteopontin (OPN), osteocalcin (OCN), and alizarin red staining was used to detect the mineralization level of cells. [Results:] The overexpression of EGR1 was observed to promote the expression of lnc-HCG11 in hPDLSCs, while the overexpression of lnc-HCG11 promoted the expression levels of RUNX2, COL1A1, OPN, OCN, and mineralization in hPDLSCs. The pro-osteogenic effect of EGR1 was inhibited by silencing lnc-HCG11, while treatment with IWR-1 suppressed the expression of active β-catenin and weakened the pro-osteogenic effect of lnc-HCG11 on hPDLSCs. [Conclusion:] EGR1 promotes osteogenic differentiation of hPDLSCs by activating the lnc-HCG11/Wnt/β-catenin pathway.https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.02.005periodontitishuman periodontal ligament stem cellsearly growth response gene 1lncrna hcg11osteogenic differentiation |
| spellingShingle | LI Cheng LONG Xiangfeng ZHOU Qin XU Fangfang HUANG Xuecheng MAO Jiatuan EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway Kouqiang hemian waike zazhi periodontitis human periodontal ligament stem cells early growth response gene 1 lncrna hcg11 osteogenic differentiation |
| title | EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway |
| title_full | EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway |
| title_fullStr | EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway |
| title_full_unstemmed | EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway |
| title_short | EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway |
| title_sort | egr1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc hcg11 wnt β catenin pathway |
| topic | periodontitis human periodontal ligament stem cells early growth response gene 1 lncrna hcg11 osteogenic differentiation |
| url | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2025.02.005 |
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