<i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>)
Understanding gene function is important for crop improvement and breeding efforts, especially in a genetically complex polyploid plant species such as the octoploid strawberry. <i>Agrobacterium</i>-mediated transient assays are a widely used tool for investigating gene functions and off...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/23/3290 |
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| author | Yoon Jeong Jang Hyeondae Han Seonghee Lee |
| author_facet | Yoon Jeong Jang Hyeondae Han Seonghee Lee |
| author_sort | Yoon Jeong Jang |
| collection | DOAJ |
| description | Understanding gene function is important for crop improvement and breeding efforts, especially in a genetically complex polyploid plant species such as the octoploid strawberry. <i>Agrobacterium</i>-mediated transient assays are a widely used tool for investigating gene functions and offer a reliable alternative to stable transformation. However, variability in tissue-specific responses and inconsistent applicability of <i>Agrobacterium</i>-mediated transient assay across diverse plant species can be challenging. In this study, we provide a method utilizing <i>Agrobacterium</i>-mediated transient expression to examine the function of genes in octoploid strawberry. Our approach encompasses leaf, root, and fruit tissues, providing a comprehensive strategy for validating gene functions in strawberries. Through meticulous optimization and validation in planta, this method offers valuable insights into gene function in strawberry functional genomics and genetics research. By addressing tissue-specific variability, our methodology serves as a valuable technical resource that could facilitate advancements in identifying gene functions in octoploid strawberry. |
| format | Article |
| id | doaj-art-c10d08ca00b04aea83148771b4389e8a |
| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-c10d08ca00b04aea83148771b4389e8a2025-08-20T02:38:42ZengMDPI AGPlants2223-77472024-11-011323329010.3390/plants13233290<i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>)Yoon Jeong Jang0Hyeondae Han1Seonghee Lee2Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Republic of KoreaVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Republic of KoreaHorticultural Sciences Department, IFAS Gulf Coast Research and Education Center, University of Florida, Wimauma, FL 33598, USAUnderstanding gene function is important for crop improvement and breeding efforts, especially in a genetically complex polyploid plant species such as the octoploid strawberry. <i>Agrobacterium</i>-mediated transient assays are a widely used tool for investigating gene functions and offer a reliable alternative to stable transformation. However, variability in tissue-specific responses and inconsistent applicability of <i>Agrobacterium</i>-mediated transient assay across diverse plant species can be challenging. In this study, we provide a method utilizing <i>Agrobacterium</i>-mediated transient expression to examine the function of genes in octoploid strawberry. Our approach encompasses leaf, root, and fruit tissues, providing a comprehensive strategy for validating gene functions in strawberries. Through meticulous optimization and validation in planta, this method offers valuable insights into gene function in strawberry functional genomics and genetics research. By addressing tissue-specific variability, our methodology serves as a valuable technical resource that could facilitate advancements in identifying gene functions in octoploid strawberry.https://www.mdpi.com/2223-7747/13/23/3290transient assays<i>Agrobacterium</i>-mediatedagroinfiltrationoctoploid strawberry |
| spellingShingle | Yoon Jeong Jang Hyeondae Han Seonghee Lee <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) Plants transient assays <i>Agrobacterium</i>-mediated agroinfiltration octoploid strawberry |
| title | <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) |
| title_full | <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) |
| title_fullStr | <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) |
| title_full_unstemmed | <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) |
| title_short | <i>Agrobacterium</i>-Mediated Transient Expression Methods to Validate Gene Functions in Strawberry (<i>F.</i> × <i>ananassa</i>) |
| title_sort | i agrobacterium i mediated transient expression methods to validate gene functions in strawberry i f i i ananassa i |
| topic | transient assays <i>Agrobacterium</i>-mediated agroinfiltration octoploid strawberry |
| url | https://www.mdpi.com/2223-7747/13/23/3290 |
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