Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties
Mushrooms of the genus Ganoderma are known for diverse biological activities, demonstrated both traditionally and experimentally. Their secondary metabolites have shown cytotoxic potential across different cancer cell lines. Besides exploration of the most active components in different species or g...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1526502/full |
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| author | Jan Šťastný Ángela Morellá-Aucejo Ángela Morellá-Aucejo Ángela Morellá-Aucejo Tomáš Skala Andrea Bernardos Andrea Bernardos Andrea Bernardos Petr Maršík Araceli Lérida-Viso Araceli Lérida-Viso Araceli Lérida-Viso Jaroslav Matějka Anna Mascellani Bergo María Dolores Marcos María Dolores Marcos María Dolores Marcos Ramón Martínez-Máñez Ramón Martínez-Máñez Ramón Martínez-Máñez Ivan Jablonský Pavel Klouček |
| author_facet | Jan Šťastný Ángela Morellá-Aucejo Ángela Morellá-Aucejo Ángela Morellá-Aucejo Tomáš Skala Andrea Bernardos Andrea Bernardos Andrea Bernardos Petr Maršík Araceli Lérida-Viso Araceli Lérida-Viso Araceli Lérida-Viso Jaroslav Matějka Anna Mascellani Bergo María Dolores Marcos María Dolores Marcos María Dolores Marcos Ramón Martínez-Máñez Ramón Martínez-Máñez Ramón Martínez-Máñez Ivan Jablonský Pavel Klouček |
| author_sort | Jan Šťastný |
| collection | DOAJ |
| description | Mushrooms of the genus Ganoderma are known for diverse biological activities, demonstrated both traditionally and experimentally. Their secondary metabolites have shown cytotoxic potential across different cancer cell lines. Besides exploration of the most active components in different species or genotypes, new formulation techniques are in development. In recent years, there has been a growing interest in the use of nanomaterials because of significant potential for pharmacology applications as substance carriers. Applying nanoparticles may enhance the medicinal effect of the mushroom substances. This study investigated the cytotoxic properties of Ganoderma species methanolic extracts against the HeLa cancer cell line. Notably, the extract obtained from Ganoderma pfeifferi demonstrated the highest activity and was further used for encapsulation within synthesized mesoporous silica nanoparticles MCM-41. Subsequently, the cytotoxic effect of the loaded MCM-41 to the free form of extract was compared. The obtained results indicate successful encapsulation, and similar activity comparing encapsulated form to free extracts (IC50 16.6 μg/mL and 20.5 μg/mL, respectively). In addition, the four unique compounds were identified as applanoxidic acid A, applanoxidic acid G, ganoderone A, and ganoderone B in the G. pfeifferi. This study is an essential prerequisite for further steps like nanoparticle functionalization for sustained or on-command delivery of these natural extracts. |
| format | Article |
| id | doaj-art-c0fc16c361294b1d97cf8679b01df1de |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-c0fc16c361294b1d97cf8679b01df1de2025-01-23T11:07:59ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011610.3389/fphar.2025.15265021526502Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic propertiesJan Šťastný0Ángela Morellá-Aucejo1Ángela Morellá-Aucejo2Ángela Morellá-Aucejo3Tomáš Skala4Andrea Bernardos5Andrea Bernardos6Andrea Bernardos7Petr Maršík8Araceli Lérida-Viso9Araceli Lérida-Viso10Araceli Lérida-Viso11Jaroslav Matějka12Anna Mascellani Bergo13María Dolores Marcos14María Dolores Marcos15María Dolores Marcos16Ramón Martínez-Máñez17Ramón Martínez-Máñez18Ramón Martínez-Máñez19Ivan Jablonský20Pavel Klouček21Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tec-nológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, SpainUnidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València-Centro de Investigación Príncipe Felipe, Valencia, SpainCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Valencia, SpainDepartment of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tec-nológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, SpainUnidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València-Centro de Investigación Príncipe Felipe, Valencia, SpainCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Valencia, SpainDepartment of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tec-nológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, SpainUnidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València-Centro de Investigación Príncipe Felipe, Valencia, SpainCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Valencia, SpainDepartment of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tec-nológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, SpainUnidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València-Centro de Investigación Príncipe Felipe, Valencia, SpainCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Valencia, SpainInstituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tec-nológico (IDM), Universitat Politècnica de València-Universitat de València, Valencia, SpainUnidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València-Centro de Investigación Príncipe Felipe, Valencia, SpainCIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Valencia, SpainDepartment of Horticulture, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaDepartment of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, CzechiaMushrooms of the genus Ganoderma are known for diverse biological activities, demonstrated both traditionally and experimentally. Their secondary metabolites have shown cytotoxic potential across different cancer cell lines. Besides exploration of the most active components in different species or genotypes, new formulation techniques are in development. In recent years, there has been a growing interest in the use of nanomaterials because of significant potential for pharmacology applications as substance carriers. Applying nanoparticles may enhance the medicinal effect of the mushroom substances. This study investigated the cytotoxic properties of Ganoderma species methanolic extracts against the HeLa cancer cell line. Notably, the extract obtained from Ganoderma pfeifferi demonstrated the highest activity and was further used for encapsulation within synthesized mesoporous silica nanoparticles MCM-41. Subsequently, the cytotoxic effect of the loaded MCM-41 to the free form of extract was compared. The obtained results indicate successful encapsulation, and similar activity comparing encapsulated form to free extracts (IC50 16.6 μg/mL and 20.5 μg/mL, respectively). In addition, the four unique compounds were identified as applanoxidic acid A, applanoxidic acid G, ganoderone A, and ganoderone B in the G. pfeifferi. This study is an essential prerequisite for further steps like nanoparticle functionalization for sustained or on-command delivery of these natural extracts.https://www.frontiersin.org/articles/10.3389/fphar.2025.1526502/fullHeLabioactivityreishi mushroomantiproliferative activityapplanoxidic acids |
| spellingShingle | Jan Šťastný Ángela Morellá-Aucejo Ángela Morellá-Aucejo Ángela Morellá-Aucejo Tomáš Skala Andrea Bernardos Andrea Bernardos Andrea Bernardos Petr Maršík Araceli Lérida-Viso Araceli Lérida-Viso Araceli Lérida-Viso Jaroslav Matějka Anna Mascellani Bergo María Dolores Marcos María Dolores Marcos María Dolores Marcos Ramón Martínez-Máñez Ramón Martínez-Máñez Ramón Martínez-Máñez Ivan Jablonský Pavel Klouček Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties Frontiers in Pharmacology HeLa bioactivity reishi mushroom antiproliferative activity applanoxidic acids |
| title | Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties |
| title_full | Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties |
| title_fullStr | Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties |
| title_full_unstemmed | Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties |
| title_short | Chemical characterization and encapsulation of Ganoderma pfeifferi extract with cytotoxic properties |
| title_sort | chemical characterization and encapsulation of ganoderma pfeifferi extract with cytotoxic properties |
| topic | HeLa bioactivity reishi mushroom antiproliferative activity applanoxidic acids |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1526502/full |
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