Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice

Abstract Dietary methionine restriction (MetR) promotes metabolic health, and we tested the impact of short durations of MetR on high fat diet (HFD)‐induced metabolic dysfunction with the maintenance of HFD. Male C57BL/6J mice were fed HFD from 10 to 25 weeks of age, then maintained on HFD or fed HF...

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Main Authors: Marissa I. McGilvrey, Bethany Fortier, Diana Cooke, Maryam A. Mahdi, Benjamin Tero, Christian M. Potts, Abigail Kaija, Larisa Ryzhova, Carolina Cora, Adam Richardson, Douglas Guzior, Ilka Pinz, Calvin Vary, Robert A. Koza, Gene Ables, Lucy Liaw
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Physiological Reports
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Online Access:https://doi.org/10.14814/phy2.70405
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author Marissa I. McGilvrey
Bethany Fortier
Diana Cooke
Maryam A. Mahdi
Benjamin Tero
Christian M. Potts
Abigail Kaija
Larisa Ryzhova
Carolina Cora
Adam Richardson
Douglas Guzior
Ilka Pinz
Calvin Vary
Robert A. Koza
Gene Ables
Lucy Liaw
author_facet Marissa I. McGilvrey
Bethany Fortier
Diana Cooke
Maryam A. Mahdi
Benjamin Tero
Christian M. Potts
Abigail Kaija
Larisa Ryzhova
Carolina Cora
Adam Richardson
Douglas Guzior
Ilka Pinz
Calvin Vary
Robert A. Koza
Gene Ables
Lucy Liaw
author_sort Marissa I. McGilvrey
collection DOAJ
description Abstract Dietary methionine restriction (MetR) promotes metabolic health, and we tested the impact of short durations of MetR on high fat diet (HFD)‐induced metabolic dysfunction with the maintenance of HFD. Male C57BL/6J mice were fed HFD from 10 to 25 weeks of age, then maintained on HFD or fed HFD with 80% reduced methionine (HFD‐MetR) for 3, 5, or 10 days. Blood, liver, adipose tissue, and aortae underwent phenotypic assessment, proteomics, and metabolomics. HFD‐MetR induced rapid weight loss and robust metabolic improvement within 10 days. Significant reductions in body weight, circulating triglycerides, glucose, insulin, adipokines and hepatokines reflected metabolic health. Proteomics revealed enriched metabolic signatures in perivascular adipose tissue (PVAT) and structural remodeling signatures in aorta. Metabolomics identified a cardioprotective signature in blood plasma, and activated mitochondrial activity and energy production in liver and brown adipose tissue. HFD‐MetR reversed metabolic dysfunction, and novel proteomic and metabolomic signatures were identified. Multi‐organ molecular changes in lipid metabolism, mitochondrial function, and bioenergetics are predicted to impact adipose tissue and liver function and cardiovascular health. Our identification of rapid changes in protein and metabolite signatures with accelerated restoration of metabolic health can be leveraged to evaluate biomarkers of metabolic health and disease in a translational context.
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spelling doaj-art-c0f90ff32486417c8ea100e2f15ec7712025-08-20T03:26:48ZengWileyPhysiological Reports2051-817X2025-06-011312n/an/a10.14814/phy2.70405Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male miceMarissa I. McGilvrey0Bethany Fortier1Diana Cooke2Maryam A. Mahdi3Benjamin Tero4Christian M. Potts5Abigail Kaija6Larisa Ryzhova7Carolina Cora8Adam Richardson9Douglas Guzior10Ilka Pinz11Calvin Vary12Robert A. Koza13Gene Ables14Lucy Liaw15Center for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USAOrentreich Foundation for the Advancement of Science, Inc. Cold Spring New York USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USAPanome Bio St. Louis Missouri USAPanome Bio St. Louis Missouri USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USAOrentreich Foundation for the Advancement of Science, Inc. Cold Spring New York USACenter for Molecular Medicine, MaineHealth Institute for Research Scarborough Maine USAAbstract Dietary methionine restriction (MetR) promotes metabolic health, and we tested the impact of short durations of MetR on high fat diet (HFD)‐induced metabolic dysfunction with the maintenance of HFD. Male C57BL/6J mice were fed HFD from 10 to 25 weeks of age, then maintained on HFD or fed HFD with 80% reduced methionine (HFD‐MetR) for 3, 5, or 10 days. Blood, liver, adipose tissue, and aortae underwent phenotypic assessment, proteomics, and metabolomics. HFD‐MetR induced rapid weight loss and robust metabolic improvement within 10 days. Significant reductions in body weight, circulating triglycerides, glucose, insulin, adipokines and hepatokines reflected metabolic health. Proteomics revealed enriched metabolic signatures in perivascular adipose tissue (PVAT) and structural remodeling signatures in aorta. Metabolomics identified a cardioprotective signature in blood plasma, and activated mitochondrial activity and energy production in liver and brown adipose tissue. HFD‐MetR reversed metabolic dysfunction, and novel proteomic and metabolomic signatures were identified. Multi‐organ molecular changes in lipid metabolism, mitochondrial function, and bioenergetics are predicted to impact adipose tissue and liver function and cardiovascular health. Our identification of rapid changes in protein and metabolite signatures with accelerated restoration of metabolic health can be leveraged to evaluate biomarkers of metabolic health and disease in a translational context.https://doi.org/10.14814/phy2.70405adipose tissuehigh fat dietmetabolomicsmethionine restrictionproteomics
spellingShingle Marissa I. McGilvrey
Bethany Fortier
Diana Cooke
Maryam A. Mahdi
Benjamin Tero
Christian M. Potts
Abigail Kaija
Larisa Ryzhova
Carolina Cora
Adam Richardson
Douglas Guzior
Ilka Pinz
Calvin Vary
Robert A. Koza
Gene Ables
Lucy Liaw
Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
Physiological Reports
adipose tissue
high fat diet
metabolomics
methionine restriction
proteomics
title Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
title_full Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
title_fullStr Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
title_full_unstemmed Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
title_short Short‐term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
title_sort short term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
topic adipose tissue
high fat diet
metabolomics
methionine restriction
proteomics
url https://doi.org/10.14814/phy2.70405
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