Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration
The purpose of our work was developing the HPLC-MS method for determining piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate and researching the presence of residual amounts of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk in 12...
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| Format: | Article |
| Language: | English |
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Zaporizhzhia State Medical and Pharmaceutical University
2019-07-01
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| Series: | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
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| Online Access: | http://pharmed.zsmu.edu.ua/article/view/171265/172665 |
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| author | B. O. Varynskyi Ye. O. Karpun V. V. Parchenko O. I. Panasenko B. P. Kyrychko І. V. Hyrenko |
| author_facet | B. O. Varynskyi Ye. O. Karpun V. V. Parchenko O. I. Panasenko B. P. Kyrychko І. V. Hyrenko |
| author_sort | B. O. Varynskyi |
| collection | DOAJ |
| description | The purpose of our work was developing the HPLC-MS method for determining piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate and researching the presence of residual amounts of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk in 12 hours after injection.
Materials and methods. Agilent 1260 Infinity Liquid Chromatography System (degasser, binary pump, autosampler, column thermostat, diode array detector, single quadrupole mass spectrometer Agilent 6120.
Results. The method of determination of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk was developed and the residual amounts of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk in 12 hours after injection was determined.
Conclusions. The conditions for sample preparation of milk samples for the determination of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)acetate have been proposed. It was found that 0.5 % methanoic acid is the most favorable protein precipitation agent. The content of the medicinal substance in the cow’s milk sample was measured in 12 hours after the injection of the 1 % solution. |
| format | Article |
| id | doaj-art-c0f5c87f309f4a24abd1d02c253ce4c6 |
| institution | Kabale University |
| issn | 2306-8094 2409-2932 |
| language | English |
| publishDate | 2019-07-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
| spelling | doaj-art-c0f5c87f309f4a24abd1d02c253ce4c62025-08-20T03:35:51ZengZaporizhzhia State Medical and Pharmaceutical UniversityAktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki2306-80942409-29322019-07-0112215315910.14739/2409-2932.2019.2.171265Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administrationB. O. VarynskyiYe. O. KarpunV. V. ParchenkoO. I. PanasenkoB. P. KyrychkoІ. V. HyrenkoThe purpose of our work was developing the HPLC-MS method for determining piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate and researching the presence of residual amounts of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk in 12 hours after injection. Materials and methods. Agilent 1260 Infinity Liquid Chromatography System (degasser, binary pump, autosampler, column thermostat, diode array detector, single quadrupole mass spectrometer Agilent 6120. Results. The method of determination of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk was developed and the residual amounts of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in cow’s milk in 12 hours after injection was determined. Conclusions. The conditions for sample preparation of milk samples for the determination of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio)acetate have been proposed. It was found that 0.5 % methanoic acid is the most favorable protein precipitation agent. The content of the medicinal substance in the cow’s milk sample was measured in 12 hours after the injection of the 1 % solution.http://pharmed.zsmu.edu.ua/article/view/171265/172665liquid chromatography-mass spectrometry14-triazoleTrifuzol-neo |
| spellingShingle | B. O. Varynskyi Ye. O. Karpun V. V. Parchenko O. I. Panasenko B. P. Kyrychko І. V. Hyrenko Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki liquid chromatography-mass spectrometry 1 4-triazole Trifuzol-neo |
| title | Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration |
| title_full | Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration |
| title_fullStr | Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration |
| title_full_unstemmed | Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration |
| title_short | Development of determination method and study of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl)thio) acetate in milk of cows in 12 hours after administration |
| title_sort | development of determination method and study of piperidinium 2 5 furan 2 yl 4 phenyl 4h 1 2 4 triazol 3 yl thio acetate in milk of cows in 12 hours after administration |
| topic | liquid chromatography-mass spectrometry 1 4-triazole Trifuzol-neo |
| url | http://pharmed.zsmu.edu.ua/article/view/171265/172665 |
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