The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques

Beta-blockers are pharmaceuticals used to treat cardiovascular diseases such as hypertension, angina pectoris, and arrhythmia. Due to high consumption, they are continuously released into the environment, being detected in many aqueous matrices. The aim of this research is to test the effectiveness...

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Main Authors: Mihail Simion Beldean-Galea, Mihaela-Cătălina Herghelegiu, Vlad-Alexandru Pănescu, Jérôme Vial, Maria Concetta Bruzzoniti, Maria-Virginia Coman
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/5/1016
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author Mihail Simion Beldean-Galea
Mihaela-Cătălina Herghelegiu
Vlad-Alexandru Pănescu
Jérôme Vial
Maria Concetta Bruzzoniti
Maria-Virginia Coman
author_facet Mihail Simion Beldean-Galea
Mihaela-Cătălina Herghelegiu
Vlad-Alexandru Pănescu
Jérôme Vial
Maria Concetta Bruzzoniti
Maria-Virginia Coman
author_sort Mihail Simion Beldean-Galea
collection DOAJ
description Beta-blockers are pharmaceuticals used to treat cardiovascular diseases such as hypertension, angina pectoris, and arrhythmia. Due to high consumption, they are continuously released into the environment, being detected in many aqueous matrices. The aim of this research is to test the effectiveness of two green liquid-phase microextraction procedures, such as dispersive liquid–liquid microextraction (DLLME) and solidification of floating organic droplet microextraction (SFOME) for the selective extraction of eight beta-blockers (atenolol, nadolol, pindolol, acebutolol, metoprolol, bisoprolol, propranolol, and betaxolol) from aqueous matrices for their analysis by gas chromatography (GC) or liquid chromatography (LC). The influence of extraction parameters, such as the type and volume of extraction and disperser solvents, and ionic strength were studied. The developed extraction procedures provide a good enrichment factor for six compounds (61.22–243.97), good extraction recovery (53.04–92.1%), and good sample cleaning for both extraction procedures. Good limits of detection (0.13 to 0.69 µg/mL for GC and 0.07 to 0.15 µg/mL for HPLC) and limits of quantification (0.39 to 2.10 µg/mL for GC and 0.20 to 0.45 µg/mL for LC) were obtained. The developed procedures were successfully applied to the analysis of selected beta-blockers in wastewater samples, proving their applicability to the real samples.
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spelling doaj-art-c0ed8feed9b94c019700b7c73b8fa9c02025-08-20T02:59:15ZengMDPI AGMolecules1420-30492025-02-01305101610.3390/molecules30051016The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic TechniquesMihail Simion Beldean-Galea0Mihaela-Cătălina Herghelegiu1Vlad-Alexandru Pănescu2Jérôme Vial3Maria Concetta Bruzzoniti4Maria-Virginia Coman5Faculty of Environmental Science and Engineering, Babeș-Bolyai University, 30 Fântânele Str., RO-400294 Cluj-Napoca, RomaniaFaculty of Environmental Science and Engineering, Babeș-Bolyai University, 30 Fântânele Str., RO-400294 Cluj-Napoca, RomaniaFaculty of Environmental Science and Engineering, Babeș-Bolyai University, 30 Fântânele Str., RO-400294 Cluj-Napoca, RomaniaChemistry, Biology and Innovation Department, École Supérieure de Physique et de Chimie Industrielles ESPCI Paris PSL, 10 Rue Vauquelin, 75005 Paris, FranceDepartment of Chemistry, University of Turin, Via P. Giuria 5, 10125 Turin, Italy“Raluca Ripan” Institute for Research in Chemistry, Babeş-Bolyai University, 30 Fântânele Str., RO-400294 Cluj-Napoca, RomaniaBeta-blockers are pharmaceuticals used to treat cardiovascular diseases such as hypertension, angina pectoris, and arrhythmia. Due to high consumption, they are continuously released into the environment, being detected in many aqueous matrices. The aim of this research is to test the effectiveness of two green liquid-phase microextraction procedures, such as dispersive liquid–liquid microextraction (DLLME) and solidification of floating organic droplet microextraction (SFOME) for the selective extraction of eight beta-blockers (atenolol, nadolol, pindolol, acebutolol, metoprolol, bisoprolol, propranolol, and betaxolol) from aqueous matrices for their analysis by gas chromatography (GC) or liquid chromatography (LC). The influence of extraction parameters, such as the type and volume of extraction and disperser solvents, and ionic strength were studied. The developed extraction procedures provide a good enrichment factor for six compounds (61.22–243.97), good extraction recovery (53.04–92.1%), and good sample cleaning for both extraction procedures. Good limits of detection (0.13 to 0.69 µg/mL for GC and 0.07 to 0.15 µg/mL for HPLC) and limits of quantification (0.39 to 2.10 µg/mL for GC and 0.20 to 0.45 µg/mL for LC) were obtained. The developed procedures were successfully applied to the analysis of selected beta-blockers in wastewater samples, proving their applicability to the real samples.https://www.mdpi.com/1420-3049/30/5/1016DLLMESFOMEbeta-blockerschromatographic techniqueswastewater samples
spellingShingle Mihail Simion Beldean-Galea
Mihaela-Cătălina Herghelegiu
Vlad-Alexandru Pănescu
Jérôme Vial
Maria Concetta Bruzzoniti
Maria-Virginia Coman
The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
Molecules
DLLME
SFOME
beta-blockers
chromatographic techniques
wastewater samples
title The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
title_full The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
title_fullStr The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
title_full_unstemmed The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
title_short The Effectiveness of Liquid-Phase Microextraction of Beta-Blockers from Aqueous Matrices for Their Analysis by Chromatographic Techniques
title_sort effectiveness of liquid phase microextraction of beta blockers from aqueous matrices for their analysis by chromatographic techniques
topic DLLME
SFOME
beta-blockers
chromatographic techniques
wastewater samples
url https://www.mdpi.com/1420-3049/30/5/1016
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