Beware of host immune responses towards bacteriophages potentially impacting phage therapy

Abstract The rising spread of antimicrobial resistance challenges animal welfare, productivity and food supply with respect to bacterial infections in different livestock species. Bacteriophages offer alternative means to control infections with resistant bacteria. This work investigated the host im...

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Main Authors: Thomas Démoulins, Jérémy D. R. Cherbuin, Thatcha Yimthin, Lukas Eggerschwiler, Fazal Adnan, Joerg Jores
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Veterinary Research
Subjects:
Online Access:https://doi.org/10.1186/s13567-025-01600-1
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author Thomas Démoulins
Jérémy D. R. Cherbuin
Thatcha Yimthin
Lukas Eggerschwiler
Fazal Adnan
Joerg Jores
author_facet Thomas Démoulins
Jérémy D. R. Cherbuin
Thatcha Yimthin
Lukas Eggerschwiler
Fazal Adnan
Joerg Jores
author_sort Thomas Démoulins
collection DOAJ
description Abstract The rising spread of antimicrobial resistance challenges animal welfare, productivity and food supply with respect to bacterial infections in different livestock species. Bacteriophages offer alternative means to control infections with resistant bacteria. This work investigated the host immune responses towards lytic bacteriophages K and T1, acting respectively on Gram-positive or Gram-negative bacteria. We employed an ex vivo platform to decipher various immune responses elicited by primary blood cells of eight outbred cattle. Bacteriophage K was not recognized whereas bacteriophage T1 induced a profound immune response. Therefore, prior characterization of host immune responses towards therapeutic phages should be considered before therapy starts.
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institution Kabale University
issn 1297-9716
language English
publishDate 2025-08-01
publisher BMC
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series Veterinary Research
spelling doaj-art-c0eacc7fa3cf44e18bde617d2b197cac2025-08-20T03:43:30ZengBMCVeterinary Research1297-97162025-08-015611810.1186/s13567-025-01600-1Beware of host immune responses towards bacteriophages potentially impacting phage therapyThomas Démoulins0Jérémy D. R. Cherbuin1Thatcha Yimthin2Lukas Eggerschwiler3Fazal Adnan4Joerg Jores5Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of BernInstitute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of BernInstitute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of BernResearch Contracts Animals GroupAtta Ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST)Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of BernAbstract The rising spread of antimicrobial resistance challenges animal welfare, productivity and food supply with respect to bacterial infections in different livestock species. Bacteriophages offer alternative means to control infections with resistant bacteria. This work investigated the host immune responses towards lytic bacteriophages K and T1, acting respectively on Gram-positive or Gram-negative bacteria. We employed an ex vivo platform to decipher various immune responses elicited by primary blood cells of eight outbred cattle. Bacteriophage K was not recognized whereas bacteriophage T1 induced a profound immune response. Therefore, prior characterization of host immune responses towards therapeutic phages should be considered before therapy starts.https://doi.org/10.1186/s13567-025-01600-1Bacteriophagesex vivo immune responsephage therapycattle
spellingShingle Thomas Démoulins
Jérémy D. R. Cherbuin
Thatcha Yimthin
Lukas Eggerschwiler
Fazal Adnan
Joerg Jores
Beware of host immune responses towards bacteriophages potentially impacting phage therapy
Veterinary Research
Bacteriophages
ex vivo immune response
phage therapy
cattle
title Beware of host immune responses towards bacteriophages potentially impacting phage therapy
title_full Beware of host immune responses towards bacteriophages potentially impacting phage therapy
title_fullStr Beware of host immune responses towards bacteriophages potentially impacting phage therapy
title_full_unstemmed Beware of host immune responses towards bacteriophages potentially impacting phage therapy
title_short Beware of host immune responses towards bacteriophages potentially impacting phage therapy
title_sort beware of host immune responses towards bacteriophages potentially impacting phage therapy
topic Bacteriophages
ex vivo immune response
phage therapy
cattle
url https://doi.org/10.1186/s13567-025-01600-1
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