Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb
Abstract Background Planar scintigraphy remains commonplace in clinical practice and has been used for quantification and dosimetry estimation over an expanding range of gamma-emitting radionuclides in recent years. Applications of planar scintigraphy, in combination with SPECT/CT imaging, can add v...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-07-01
|
| Series: | EJNMMI Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40658-025-00775-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849334341114527744 |
|---|---|
| author | John D. Wright Isaline Renard Isis A. Middleton Juozas Domarkas Émer M. Foyle Paul J. Lusby Stephen J. Archibald |
| author_facet | John D. Wright Isaline Renard Isis A. Middleton Juozas Domarkas Émer M. Foyle Paul J. Lusby Stephen J. Archibald |
| author_sort | John D. Wright |
| collection | DOAJ |
| description | Abstract Background Planar scintigraphy remains commonplace in clinical practice and has been used for quantification and dosimetry estimation over an expanding range of gamma-emitting radionuclides in recent years. Applications of planar scintigraphy, in combination with SPECT/CT imaging, can add value to radiopharmaceutical development in preclinical models and in translation to human use. The aim of this study was to demonstrate whole-body quantitative accuracy in mice using pinhole collimated planar scintigraphy on a preclinical SPECT/CT system, following corrections to sensitivity variations across the field of view. Results Planar projections were acquired using short imaging time frames, thus allowing for dynamic biodistribution data to be collected and compared to the known injected activity and whole-body SPECT data. Encapsulation of [99mTc]TcO4 – in a supramolecular cage was used to demonstrate the visual and quantitative changes in biodistribution over time, as compared to [99mTc]TcO4 – alone. For these radiopharmaceuticals, whole-body quantification was 98.7 ± 7.3% of the decay-corrected true injected activity, as opposed to 74.8 ± 7.5% when calculated without a sensitivity correction. Similarly, the final planar scintigraphy frame acquired at 1-hour post-injection quantitatively agreed with activity values returned from the whole-body SPECT: 99.5 ± 10.6% (final frame, planar) vs. 99.1 ± 5.5% (SPECT). Regions of interest (ROIs) over selected organs between planar scintigraphy and SPECT were also in good agreement. Quantitative accuracy of planar scintigraphy was further validated in a preclinical tumour model of prostate cancer using [161Tb]Tb-PSMA-617. In this case, the whole-body planar value was 94.6 ± 3.6% of the recorded injected activity and, consistent with 99mTc findings, was underestimated without sensitivity correction (76.6 ± 3.1%). Tumour uptake values were equivalent between corrected planar scintigraphy (5.2%IA) and SPECT (5.3%IA) at 1-hour post-injection. Conclusions Using a common radionuclide and one of emerging radiotherapeutic interest, whole-body injected activity and organ-specific ROI values obtained by planar scintigraphy strongly correlated to the true injected activity and values obtained by SPECT following sensitivity-based corrections. The addition of quantitative dynamic planar scintigraphy into the preclinical workflow followed by SPECT imaging adds value to pharmacokinetic and dosimetry assessments of novel gamma-emitting radiopharmaceuticals in imaging and therapeutic applications. |
| format | Article |
| id | doaj-art-c0e40952f4ea4ecd91cc521187a32f84 |
| institution | Kabale University |
| issn | 2197-7364 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Physics |
| spelling | doaj-art-c0e40952f4ea4ecd91cc521187a32f842025-08-20T03:45:35ZengSpringerOpenEJNMMI Physics2197-73642025-07-0112111510.1186/s40658-025-00775-yQuantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161TbJohn D. Wright0Isaline Renard1Isis A. Middleton2Juozas Domarkas3Émer M. Foyle4Paul J. Lusby5Stephen J. Archibald6School of Biomedical Engineering and Imaging Sciences, King’s College London, St Thomas’ HospitalSchool of Biomedical Engineering and Imaging Sciences, King’s College London, St Thomas’ HospitalEaStCHEM School of Chemistry, University of EdinburghCentre for Biomedicine and PET Research Centre, Hull York Medical School, University of HullEaStCHEM School of Chemistry, University of EdinburghEaStCHEM School of Chemistry, University of EdinburghSchool of Biomedical Engineering and Imaging Sciences, King’s College London, St Thomas’ HospitalAbstract Background Planar scintigraphy remains commonplace in clinical practice and has been used for quantification and dosimetry estimation over an expanding range of gamma-emitting radionuclides in recent years. Applications of planar scintigraphy, in combination with SPECT/CT imaging, can add value to radiopharmaceutical development in preclinical models and in translation to human use. The aim of this study was to demonstrate whole-body quantitative accuracy in mice using pinhole collimated planar scintigraphy on a preclinical SPECT/CT system, following corrections to sensitivity variations across the field of view. Results Planar projections were acquired using short imaging time frames, thus allowing for dynamic biodistribution data to be collected and compared to the known injected activity and whole-body SPECT data. Encapsulation of [99mTc]TcO4 – in a supramolecular cage was used to demonstrate the visual and quantitative changes in biodistribution over time, as compared to [99mTc]TcO4 – alone. For these radiopharmaceuticals, whole-body quantification was 98.7 ± 7.3% of the decay-corrected true injected activity, as opposed to 74.8 ± 7.5% when calculated without a sensitivity correction. Similarly, the final planar scintigraphy frame acquired at 1-hour post-injection quantitatively agreed with activity values returned from the whole-body SPECT: 99.5 ± 10.6% (final frame, planar) vs. 99.1 ± 5.5% (SPECT). Regions of interest (ROIs) over selected organs between planar scintigraphy and SPECT were also in good agreement. Quantitative accuracy of planar scintigraphy was further validated in a preclinical tumour model of prostate cancer using [161Tb]Tb-PSMA-617. In this case, the whole-body planar value was 94.6 ± 3.6% of the recorded injected activity and, consistent with 99mTc findings, was underestimated without sensitivity correction (76.6 ± 3.1%). Tumour uptake values were equivalent between corrected planar scintigraphy (5.2%IA) and SPECT (5.3%IA) at 1-hour post-injection. Conclusions Using a common radionuclide and one of emerging radiotherapeutic interest, whole-body injected activity and organ-specific ROI values obtained by planar scintigraphy strongly correlated to the true injected activity and values obtained by SPECT following sensitivity-based corrections. The addition of quantitative dynamic planar scintigraphy into the preclinical workflow followed by SPECT imaging adds value to pharmacokinetic and dosimetry assessments of novel gamma-emitting radiopharmaceuticals in imaging and therapeutic applications.https://doi.org/10.1186/s40658-025-00775-yQuantificationDynamicPlanar scintigraphySPECTGamma cameraPreclinical |
| spellingShingle | John D. Wright Isaline Renard Isis A. Middleton Juozas Domarkas Émer M. Foyle Paul J. Lusby Stephen J. Archibald Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb EJNMMI Physics Quantification Dynamic Planar scintigraphy SPECT Gamma camera Preclinical |
| title | Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb |
| title_full | Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb |
| title_fullStr | Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb |
| title_full_unstemmed | Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb |
| title_short | Quantitative whole-body dynamic planar scintigraphy in mice with 99mTc and 161Tb |
| title_sort | quantitative whole body dynamic planar scintigraphy in mice with 99mtc and 161tb |
| topic | Quantification Dynamic Planar scintigraphy SPECT Gamma camera Preclinical |
| url | https://doi.org/10.1186/s40658-025-00775-y |
| work_keys_str_mv | AT johndwright quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT isalinerenard quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT isisamiddleton quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT juozasdomarkas quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT emermfoyle quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT pauljlusby quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb AT stephenjarchibald quantitativewholebodydynamicplanarscintigraphyinmicewith99mtcand161tb |