Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes
Mechanically activated (MA) Piezo1 channels play an important role in both normal physiology and pathological dysfunction in multiple tissues and organs. In skeletal muscle cells, Piezo1 channels are involved in the regulation of postnatal myogenesis and muscle regeneration after injury. To further...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.70008 |
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| author | Natalia A. Vilchinskaya Ksenia V. Sergeeva Boris S. Shenkman Timur M. Mirzoev |
| author_facet | Natalia A. Vilchinskaya Ksenia V. Sergeeva Boris S. Shenkman Timur M. Mirzoev |
| author_sort | Natalia A. Vilchinskaya |
| collection | DOAJ |
| description | Mechanically activated (MA) Piezo1 channels play an important role in both normal physiology and pathological dysfunction in multiple tissues and organs. In skeletal muscle cells, Piezo1 channels are involved in the regulation of postnatal myogenesis and muscle regeneration after injury. To further understand the role of MA Piezo1 channels as potential critical sensors of mechanical perturbations during muscle contractions, we studied the possible contribution of MA Piezo1 channels to enhanced protein synthetic response of C2C12 myotubes to mechanical simulation. C2C12 myotubes were subjected to mechanical stimulation by electrical pulse stimulation (EPS) alone or EPS in combination with Yoda1, Gadolinium or Yoda1 + Gadolinium. EPS alone elicited an increase in anabolic signaling and protein synthesis (PS). Incubation with Yoda1 during EPS enhanced anabolic signaling and PS compared to EPS alone. Gadolinium or Yoda1 + gadolinium during EPS abolished or diminished the Yoda1 + EPS‐induced effects on anabolic signaling and PS. Our work demonstrates that chemical activation of Piezo1 channels during mechanical stimulation contributes to enhanced protein anabolism in C2C12 myotubes. |
| format | Article |
| id | doaj-art-c0d9b2a98bde477ba6fd429efbc3fdec |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-c0d9b2a98bde477ba6fd429efbc3fdec2025-08-20T01:57:14ZengWileyFEBS Open Bio2211-54632025-06-0115694094810.1002/2211-5463.70008Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubesNatalia A. Vilchinskaya0Ksenia V. Sergeeva1Boris S. Shenkman2Timur M. Mirzoev3Myology Laboratory Institute of Biomedical Problems of the Russian Academy of Sciences Moscow RussiaMyology Laboratory Institute of Biomedical Problems of the Russian Academy of Sciences Moscow RussiaMyology Laboratory Institute of Biomedical Problems of the Russian Academy of Sciences Moscow RussiaMyology Laboratory Institute of Biomedical Problems of the Russian Academy of Sciences Moscow RussiaMechanically activated (MA) Piezo1 channels play an important role in both normal physiology and pathological dysfunction in multiple tissues and organs. In skeletal muscle cells, Piezo1 channels are involved in the regulation of postnatal myogenesis and muscle regeneration after injury. To further understand the role of MA Piezo1 channels as potential critical sensors of mechanical perturbations during muscle contractions, we studied the possible contribution of MA Piezo1 channels to enhanced protein synthetic response of C2C12 myotubes to mechanical simulation. C2C12 myotubes were subjected to mechanical stimulation by electrical pulse stimulation (EPS) alone or EPS in combination with Yoda1, Gadolinium or Yoda1 + Gadolinium. EPS alone elicited an increase in anabolic signaling and protein synthesis (PS). Incubation with Yoda1 during EPS enhanced anabolic signaling and PS compared to EPS alone. Gadolinium or Yoda1 + gadolinium during EPS abolished or diminished the Yoda1 + EPS‐induced effects on anabolic signaling and PS. Our work demonstrates that chemical activation of Piezo1 channels during mechanical stimulation contributes to enhanced protein anabolism in C2C12 myotubes.https://doi.org/10.1002/2211-5463.70008C2C12 myotubeselectrical pulse stimulationgadoliniumPiezo1protein synthesisYoda1 |
| spellingShingle | Natalia A. Vilchinskaya Ksenia V. Sergeeva Boris S. Shenkman Timur M. Mirzoev Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes FEBS Open Bio C2C12 myotubes electrical pulse stimulation gadolinium Piezo1 protein synthesis Yoda1 |
| title | Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| title_full | Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| title_fullStr | Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| title_full_unstemmed | Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| title_short | Piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| title_sort | piezo1 channels enhance anabolic signaling activation induced by electrical stimulation of cultured myotubes |
| topic | C2C12 myotubes electrical pulse stimulation gadolinium Piezo1 protein synthesis Yoda1 |
| url | https://doi.org/10.1002/2211-5463.70008 |
| work_keys_str_mv | AT nataliaavilchinskaya piezo1channelsenhanceanabolicsignalingactivationinducedbyelectricalstimulationofculturedmyotubes AT kseniavsergeeva piezo1channelsenhanceanabolicsignalingactivationinducedbyelectricalstimulationofculturedmyotubes AT borissshenkman piezo1channelsenhanceanabolicsignalingactivationinducedbyelectricalstimulationofculturedmyotubes AT timurmmirzoev piezo1channelsenhanceanabolicsignalingactivationinducedbyelectricalstimulationofculturedmyotubes |