High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo
Salinity is a major environmental stress limiting crop growth and yield in many arid and semi-arid regions of the world. In this study, we used a germination-based selection method to screen an EMS collection of Cucurbita pepo, consisting of 3,751 M2 lines, for salt tolerance. The screening resulted...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1548576/full |
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| author | Sonsoles Alonso Keshav Gautam Jessica Iglesias-Moya María Segura María del Mar Rebolloso María del Carmen Salas-Sanjuán Cecilia Martínez Manuel Jamilena |
| author_facet | Sonsoles Alonso Keshav Gautam Jessica Iglesias-Moya María Segura María del Mar Rebolloso María del Carmen Salas-Sanjuán Cecilia Martínez Manuel Jamilena |
| author_sort | Sonsoles Alonso |
| collection | DOAJ |
| description | Salinity is a major environmental stress limiting crop growth and yield in many arid and semi-arid regions of the world. In this study, we used a germination-based selection method to screen an EMS collection of Cucurbita pepo, consisting of 3,751 M2 lines, for salt tolerance. The screening resulted in the identification of six salt-tolerant mutants that exhibited enhanced germination and plant growth under salt stress conditions. This enhanced salt tolerance was found to be associated with increased production of proline, soluble sugars, and anthocyanins, which are known to exert osmoprotective and ROS scavenging functions. The mutant lines TS-1378 and TS-2075 were selected to be tested as rootstocks and showed a positive effect on the vegetative growth of scions under standard and saline conditions. In conclusion, the research provides an efficient protocol for high throughput screening for salt tolerance, novel useful mutants to study the mechanism behind salt tolerance, and valuable genetic resources for pumpkin breeding. |
| format | Article |
| id | doaj-art-c0ca98bd2bf44fa593a522266049ec06 |
| institution | DOAJ |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-c0ca98bd2bf44fa593a522266049ec062025-08-20T03:10:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15485761548576High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepoSonsoles Alonso0Keshav Gautam1Jessica Iglesias-Moya2María Segura3María del Mar Rebolloso4María del Carmen Salas-Sanjuán5Cecilia Martínez6Manuel Jamilena7Department of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainDepartment of Agronomy, and CIAIMBITAL Research Center. University of Almería, Almería, SpainDepartment of Agronomy, and CIAIMBITAL Research Center. University of Almería, Almería, SpainDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainDepartment of Biology and Geology, Agrifood Campus of International Excellence (CeiA3), and Research Center CIAMBITAL, University of Almería, Almería, SpainSalinity is a major environmental stress limiting crop growth and yield in many arid and semi-arid regions of the world. In this study, we used a germination-based selection method to screen an EMS collection of Cucurbita pepo, consisting of 3,751 M2 lines, for salt tolerance. The screening resulted in the identification of six salt-tolerant mutants that exhibited enhanced germination and plant growth under salt stress conditions. This enhanced salt tolerance was found to be associated with increased production of proline, soluble sugars, and anthocyanins, which are known to exert osmoprotective and ROS scavenging functions. The mutant lines TS-1378 and TS-2075 were selected to be tested as rootstocks and showed a positive effect on the vegetative growth of scions under standard and saline conditions. In conclusion, the research provides an efficient protocol for high throughput screening for salt tolerance, novel useful mutants to study the mechanism behind salt tolerance, and valuable genetic resources for pumpkin breeding.https://www.frontiersin.org/articles/10.3389/fpls.2025.1548576/fullCucurbita peposcreeningsalt stressgerminationEMSmutants |
| spellingShingle | Sonsoles Alonso Keshav Gautam Jessica Iglesias-Moya María Segura María del Mar Rebolloso María del Carmen Salas-Sanjuán Cecilia Martínez Manuel Jamilena High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo Frontiers in Plant Science Cucurbita pepo screening salt stress germination EMS mutants |
| title | High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo |
| title_full | High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo |
| title_fullStr | High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo |
| title_full_unstemmed | High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo |
| title_short | High throughput screening and evaluation of salt-tolerant mutants from an EMS collection of Cucurbita pepo |
| title_sort | high throughput screening and evaluation of salt tolerant mutants from an ems collection of cucurbita pepo |
| topic | Cucurbita pepo screening salt stress germination EMS mutants |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1548576/full |
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