Identification of novel mobile colistin resistance gene mcr-10
Mobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1732231 |
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| author | Chengcheng Wang Yu Feng Lina Liu Li Wei Mei Kang Zhiyong Zong |
| author_facet | Chengcheng Wang Yu Feng Lina Liu Li Wei Mei Kang Zhiyong Zong |
| author_sort | Chengcheng Wang |
| collection | DOAJ |
| description | Mobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical to MCR-9. mcr-10 confers 4-fold increase in colistin MIC (from 1 to 4 mg/L) when cloned into a colistin-susceptible E. roggenkampii strain. By screening GenBank, mcr-10 was found in various Enterobacteriaceae species of countries in four continents, suggesting that this gene has widely spread. MCR-10 shows 79.04% to 83.67% amino acid identity and highly conserved predicted protein structures with chromosomally encoded MCR-like phosphoethanolamine transferases (designated MCR-B here) of various Buttiauxella species. MCR-10, MCR-9 and MCR-B proteins may, therefore, originate from a common ancestor. mcr-10 was adjacent to a site-specific recombinase-encoding gene and was bracketed by IS903 and may be mobilized by site-specific recombination or composite transposon. Our results indicate that mcr-10 is a novel plasmid-borne colistin resistance gene and warrants immediate monitoring and further studies. |
| format | Article |
| id | doaj-art-c0c774b757ab4e24a350a77d94552fb4 |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-c0c774b757ab4e24a350a77d94552fb42025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019150851610.1080/22221751.2020.1732231Identification of novel mobile colistin resistance gene mcr-10Chengcheng Wang0Yu Feng1Lina Liu2Li Wei3Mei Kang4Zhiyong Zong5Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaCenter of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaCenter for Pathogen Research, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaDepartment of Infection Control, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaLaboratory of Clinical Microbiology, Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaCenter of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, People’s Republic of ChinaMobile colistin resistance (mcr) genes represent an emerging challenge. Here we describe a novel mcr gene, mcr-10, on an IncFIA plasmid of an Enterobacter roggenkampii clinical strain. mcr-10 has the highest nucleotide identity (79.69%) with mcr-9 and encodes MCR-10 with 82.93% amino acids identical to MCR-9. mcr-10 confers 4-fold increase in colistin MIC (from 1 to 4 mg/L) when cloned into a colistin-susceptible E. roggenkampii strain. By screening GenBank, mcr-10 was found in various Enterobacteriaceae species of countries in four continents, suggesting that this gene has widely spread. MCR-10 shows 79.04% to 83.67% amino acid identity and highly conserved predicted protein structures with chromosomally encoded MCR-like phosphoethanolamine transferases (designated MCR-B here) of various Buttiauxella species. MCR-10, MCR-9 and MCR-B proteins may, therefore, originate from a common ancestor. mcr-10 was adjacent to a site-specific recombinase-encoding gene and was bracketed by IS903 and may be mobilized by site-specific recombination or composite transposon. Our results indicate that mcr-10 is a novel plasmid-borne colistin resistance gene and warrants immediate monitoring and further studies.https://www.tandfonline.com/doi/10.1080/22221751.2020.1732231Colistin resistancemcrmcr-10plasmidEnterobacter roggenkampii |
| spellingShingle | Chengcheng Wang Yu Feng Lina Liu Li Wei Mei Kang Zhiyong Zong Identification of novel mobile colistin resistance gene mcr-10 Emerging Microbes and Infections Colistin resistance mcr mcr-10 plasmid Enterobacter roggenkampii |
| title | Identification of novel mobile colistin resistance gene mcr-10 |
| title_full | Identification of novel mobile colistin resistance gene mcr-10 |
| title_fullStr | Identification of novel mobile colistin resistance gene mcr-10 |
| title_full_unstemmed | Identification of novel mobile colistin resistance gene mcr-10 |
| title_short | Identification of novel mobile colistin resistance gene mcr-10 |
| title_sort | identification of novel mobile colistin resistance gene mcr 10 |
| topic | Colistin resistance mcr mcr-10 plasmid Enterobacter roggenkampii |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1732231 |
| work_keys_str_mv | AT chengchengwang identificationofnovelmobilecolistinresistancegenemcr10 AT yufeng identificationofnovelmobilecolistinresistancegenemcr10 AT linaliu identificationofnovelmobilecolistinresistancegenemcr10 AT liwei identificationofnovelmobilecolistinresistancegenemcr10 AT meikang identificationofnovelmobilecolistinresistancegenemcr10 AT zhiyongzong identificationofnovelmobilecolistinresistancegenemcr10 |