The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes
Pepper is one of the most cultivated vegetables worldwide and also consumed substantially as a flavouring ingredient in different culinary traditions. Therefore, many researchers have focused on its breeding to develop new varieties. One of plant breeding aims is to attain disease and pest resistanc...
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Hasan Eleroğlu
2019-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2828 |
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| author | Flavien Shimira Davut Keleş Hatıra Taşkın Kazım Abak |
| author_facet | Flavien Shimira Davut Keleş Hatıra Taşkın Kazım Abak |
| author_sort | Flavien Shimira |
| collection | DOAJ |
| description | Pepper is one of the most cultivated vegetables worldwide and also consumed substantially as a flavouring ingredient in different culinary traditions. Therefore, many researchers have focused on its breeding to develop new varieties. One of plant breeding aims is to attain disease and pest resistance. The use of tissue culture methods in plant breeding has many advantages. The response of two nematode resistant pepper genotypes to the anther culture and the effect of cold pre-treatment to the floral buds have been investigated in this study. Alata 2095 and Alata 2096 both specified as resistant to the nematode by Alata Horticultural Research Institute (Alata, Mersin, Turkey) were used as plant material. Two pre-treatments were used in this study: cold and no cold. In cold treatment, flower buds were kept in fridge at 4°C for 24 hours prior to the anther culture. Murashige and Skoog medium contained 0.25% activated charcoal, 6.5 g L-1 agar, 0.5 mg L-1 6-benzyl-amino-purine (BAP), 4 mg L-1 naphthalene-acetic-acid (NAA), 15 mg L-1 silver nitrate (AgNO3), and 30 g L-1 sucrose was used. After assessment, the highest mean of plant number was 39.08 per 100 anthers for Alata 2095 genotype. It was 46.61 and 31.56 in cold and no cold treatment, respectively. For Alata 2096 genotype, the mean was 1.96 per 100 anthers (1.68 and 2.25 in cold and no cold treatment, respectively). Statistical analyses confirmed that there was significant difference between treatments, genotypes and also significant interaction between those factors. At the end of the study, we can say that Alata 2095 genotype has a good androgenic response and it can be beneficial in further pepper breeding studies. |
| format | Article |
| id | doaj-art-c0c0ee519b7a40cab7e8eaa0d023c0d5 |
| institution | OA Journals |
| issn | 2148-127X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-c0c0ee519b7a40cab7e8eaa0d023c0d52025-08-20T02:02:29ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2019-12-017122103211010.24925/turjaf.v7i12.2103-2110.28281381The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) GenotypesFlavien Shimira0Davut Keleş1Hatıra Taşkın2Kazım Abak3Department of Horticultural Production and Marketing, Faculty of Agricultural Sciences and Technologies, European University of Lefke, Lefke, Department of Horticulture, Faculty of Agriculture, Cukurova University, Adana, TurkeyAlata Horticultural Research Institute, Erdemli-MersinDepartment of Horticulture, Faculty of Agriculture, Cukurova University, AdanaDepartment of Horticultural Production and Marketing, Faculty of Agricultural Sciences and Technologies, European University of Lefke, LefkePepper is one of the most cultivated vegetables worldwide and also consumed substantially as a flavouring ingredient in different culinary traditions. Therefore, many researchers have focused on its breeding to develop new varieties. One of plant breeding aims is to attain disease and pest resistance. The use of tissue culture methods in plant breeding has many advantages. The response of two nematode resistant pepper genotypes to the anther culture and the effect of cold pre-treatment to the floral buds have been investigated in this study. Alata 2095 and Alata 2096 both specified as resistant to the nematode by Alata Horticultural Research Institute (Alata, Mersin, Turkey) were used as plant material. Two pre-treatments were used in this study: cold and no cold. In cold treatment, flower buds were kept in fridge at 4°C for 24 hours prior to the anther culture. Murashige and Skoog medium contained 0.25% activated charcoal, 6.5 g L-1 agar, 0.5 mg L-1 6-benzyl-amino-purine (BAP), 4 mg L-1 naphthalene-acetic-acid (NAA), 15 mg L-1 silver nitrate (AgNO3), and 30 g L-1 sucrose was used. After assessment, the highest mean of plant number was 39.08 per 100 anthers for Alata 2095 genotype. It was 46.61 and 31.56 in cold and no cold treatment, respectively. For Alata 2096 genotype, the mean was 1.96 per 100 anthers (1.68 and 2.25 in cold and no cold treatment, respectively). Statistical analyses confirmed that there was significant difference between treatments, genotypes and also significant interaction between those factors. At the end of the study, we can say that Alata 2095 genotype has a good androgenic response and it can be beneficial in further pepper breeding studies.http://www.agrifoodscience.com/index.php/TURJAF/article/view/2828anther culturegenotypehaploid plant productionnematode resistancecold pre-treatment |
| spellingShingle | Flavien Shimira Davut Keleş Hatıra Taşkın Kazım Abak The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes Turkish Journal of Agriculture: Food Science and Technology anther culture genotype haploid plant production nematode resistance cold pre-treatment |
| title | The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes |
| title_full | The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes |
| title_fullStr | The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes |
| title_full_unstemmed | The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes |
| title_short | The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes |
| title_sort | assessment of androgenic response of two nematode resistant pepper capsicum annuum l genotypes |
| topic | anther culture genotype haploid plant production nematode resistance cold pre-treatment |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2828 |
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