Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients
The knowledge on how gut microbes contribute to the inflammatory bowel disease (IBD) at the onset of disease is still scarce. We compared gut microbiota in newly diagnosed, treatment-naïve adult IBD (Crohn’s disease (CD) and ulcerative colitis (UC)) to irritable bowel syndrome (IBS) patients and hea...
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| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2083419 |
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| author | Hana Čipčić Paljetak Anja Barešić Marina Panek Mihaela Perić Mario Matijašić Ivana Lojkić Ana Barišić Darija Vranešić Bender Dina Ljubas Kelečić Marko Brinar Mirjana Kalauz Marija Miličević Dora Grgić Nikša Turk Irena Karas Silvija Čuković-Čavka Željko Krznarić Donatella Verbanac |
| author_facet | Hana Čipčić Paljetak Anja Barešić Marina Panek Mihaela Perić Mario Matijašić Ivana Lojkić Ana Barišić Darija Vranešić Bender Dina Ljubas Kelečić Marko Brinar Mirjana Kalauz Marija Miličević Dora Grgić Nikša Turk Irena Karas Silvija Čuković-Čavka Željko Krznarić Donatella Verbanac |
| author_sort | Hana Čipčić Paljetak |
| collection | DOAJ |
| description | The knowledge on how gut microbes contribute to the inflammatory bowel disease (IBD) at the onset of disease is still scarce. We compared gut microbiota in newly diagnosed, treatment-naïve adult IBD (Crohn’s disease (CD) and ulcerative colitis (UC)) to irritable bowel syndrome (IBS) patients and healthy group. Mucosal and fecal microbiota of 49 patients (13 UC, 10 CD, and 26 IBS) before treatment initiation, and fecal microbiota of 12 healthy subjects was characterized by 16S rRNA gene sequencing. Mucosa was sampled at six positions, from terminal ileum to rectum. We demonstrate that mucosal microbiota is spatially homogeneous, cannot be differentiated based on the local inflammation status and yet provides bacterial footprints superior to fecal in discriminating disease phenotypes. IBD groups showed decreased bacterial diversity in mucosa at all taxonomic levels compared to IBS. In CD and UC, Dialister was significantly increased, and expansion of Haemophilus and Propionibacterium characterized UC. Compared to healthy individuals, fecal microbiota of IBD and IBS patients had increased abundance of Proteobacteria, Enterobacteriaceae, in particular. Shift toward reduction of Adlercreutzia and butyrate-producing taxa was found in feces of IBD patients. Microbiota alterations detected in newly diagnosed treatment-naïve adult patients indicate that the microbiota changes are set and detectable at the disease onset and likely have a discerning role in IBD pathophysiology. Our results justify further investigation of the taxa discriminating between disease groups, such as H. parainfluenzae, R. gnavus, Turicibacteriaceae, Dialister, and Adlercreutzia as potential biomarkers of the disease. |
| format | Article |
| id | doaj-art-c0c0c6fbc82d45b0a95de343b7ea55e1 |
| institution | DOAJ |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-c0c0c6fbc82d45b0a95de343b7ea55e12025-08-20T03:22:16ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2083419Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patientsHana Čipčić Paljetak0Anja Barešić1Marina Panek2Mihaela Perić3Mario Matijašić4Ivana Lojkić5Ana Barišić6Darija Vranešić Bender7Dina Ljubas Kelečić8Marko Brinar9Mirjana Kalauz10Marija Miličević11Dora Grgić12Nikša Turk13Irena Karas14Silvija Čuković-Čavka15Željko Krznarić16Donatella Verbanac17Center for Translational and Clinical Research, University of Zagreb School of Medicine, Zagreb, CroatiaDivision of Electronics, Ruđer Bošković Institute, Zagreb, CroatiaCenter for Translational and Clinical Research, University of Zagreb School of Medicine, Zagreb, CroatiaCenter for Translational and Clinical Research, University of Zagreb School of Medicine, Zagreb, CroatiaCenter for Translational and Clinical Research, University of Zagreb School of Medicine, Zagreb, CroatiaDepartment for Virology, Croatian Veterinary Institute, Zagreb, CroatiaDepartment of Internal Medicine, Unit of Clinical Nutrition, University Hospital Centre Zagreb, Zagreb, CroatiaDepartment of Internal Medicine, Unit of Clinical Nutrition, University Hospital Centre Zagreb, Zagreb, CroatiaDepartment of Internal Medicine, Unit of Clinical Nutrition, University Hospital Centre Zagreb, Zagreb, CroatiaUniversity of Zagreb School of Medicine, Zagreb, CroatiaUniversity of Zagreb School of Medicine, Zagreb, CroatiaUniversity of Zagreb School of Medicine, Zagreb, CroatiaDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University Hospital Centre Zagreb, Zagreb, CroatiaDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University Hospital Centre Zagreb, Zagreb, CroatiaDepartment of Internal Medicine, Unit of Clinical Nutrition, University Hospital Centre Zagreb, Zagreb, CroatiaUniversity of Zagreb School of Medicine, Zagreb, CroatiaDepartment of Internal Medicine, Unit of Clinical Nutrition, University Hospital Centre Zagreb, Zagreb, CroatiaCenter for Translational and Clinical Research, University of Zagreb School of Medicine, Zagreb, CroatiaThe knowledge on how gut microbes contribute to the inflammatory bowel disease (IBD) at the onset of disease is still scarce. We compared gut microbiota in newly diagnosed, treatment-naïve adult IBD (Crohn’s disease (CD) and ulcerative colitis (UC)) to irritable bowel syndrome (IBS) patients and healthy group. Mucosal and fecal microbiota of 49 patients (13 UC, 10 CD, and 26 IBS) before treatment initiation, and fecal microbiota of 12 healthy subjects was characterized by 16S rRNA gene sequencing. Mucosa was sampled at six positions, from terminal ileum to rectum. We demonstrate that mucosal microbiota is spatially homogeneous, cannot be differentiated based on the local inflammation status and yet provides bacterial footprints superior to fecal in discriminating disease phenotypes. IBD groups showed decreased bacterial diversity in mucosa at all taxonomic levels compared to IBS. In CD and UC, Dialister was significantly increased, and expansion of Haemophilus and Propionibacterium characterized UC. Compared to healthy individuals, fecal microbiota of IBD and IBS patients had increased abundance of Proteobacteria, Enterobacteriaceae, in particular. Shift toward reduction of Adlercreutzia and butyrate-producing taxa was found in feces of IBD patients. Microbiota alterations detected in newly diagnosed treatment-naïve adult patients indicate that the microbiota changes are set and detectable at the disease onset and likely have a discerning role in IBD pathophysiology. Our results justify further investigation of the taxa discriminating between disease groups, such as H. parainfluenzae, R. gnavus, Turicibacteriaceae, Dialister, and Adlercreutzia as potential biomarkers of the disease.https://www.tandfonline.com/doi/10.1080/19490976.2022.2083419Gut microbiotagut mucosafecal microbiotainflammatory bowel diseaseCrohn’s diseaseulcerative colitis |
| spellingShingle | Hana Čipčić Paljetak Anja Barešić Marina Panek Mihaela Perić Mario Matijašić Ivana Lojkić Ana Barišić Darija Vranešić Bender Dina Ljubas Kelečić Marko Brinar Mirjana Kalauz Marija Miličević Dora Grgić Nikša Turk Irena Karas Silvija Čuković-Čavka Željko Krznarić Donatella Verbanac Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients Gut Microbes Gut microbiota gut mucosa fecal microbiota inflammatory bowel disease Crohn’s disease ulcerative colitis |
| title | Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients |
| title_full | Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients |
| title_fullStr | Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients |
| title_full_unstemmed | Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients |
| title_short | Gut microbiota in mucosa and feces of newly diagnosed, treatment-naïve adult inflammatory bowel disease and irritable bowel syndrome patients |
| title_sort | gut microbiota in mucosa and feces of newly diagnosed treatment naive adult inflammatory bowel disease and irritable bowel syndrome patients |
| topic | Gut microbiota gut mucosa fecal microbiota inflammatory bowel disease Crohn’s disease ulcerative colitis |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2083419 |
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