Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR)
Solid-state <sup>13</sup>C NMR spectroscopy using cross-polarization magic-angle spinning is a highly valuable technique for the semi-quantitative analysis of complex solid matrices. One of its key advantages is that it does not require any manipulation of the matrix, such as extractions...
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MDPI AG
2025-06-01
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| Series: | Seeds |
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| author | Stefania Mazzini Marco Zuccolo Angela Bassoli Claudio Gardana Gigliola Borgonovo |
| author_facet | Stefania Mazzini Marco Zuccolo Angela Bassoli Claudio Gardana Gigliola Borgonovo |
| author_sort | Stefania Mazzini |
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| description | Solid-state <sup>13</sup>C NMR spectroscopy using cross-polarization magic-angle spinning is a highly valuable technique for the semi-quantitative analysis of complex solid matrices. One of its key advantages is that it does not require any manipulation of the matrix, such as extractions or other treatments, which is particularly important for preserving the integrity of unstable secondary metabolites. Glucosinolates (β-thioglucoside-N-hydrosulphates) are crucial secondary metabolites specific to <i>Brassica</i> species, and many of them are known to be highly unstable. In this study, we evaluated solid-state nuclear magnetic resonance spectroscopy as an alternative method for the identification and quantification of total glucosinolates in the seeds of <i>Sisymbrium officinale</i>, <i>Brassica napus</i>, <i>Sinapis alba</i>, <i>Brassica nigra</i>, and <i>Moringa oleifera</i>. The results obtained with this method showed good agreement with those from conventional chemical analyses of the seed material. Although, based on a limited number of samples, this preliminary study suggests that the proposed approach could be a useful alternative for quantifying total glucosinolate content in seeds. |
| format | Article |
| id | doaj-art-c0ba0e953902480090db948ef64fa9aa |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Seeds |
| spelling | doaj-art-c0ba0e953902480090db948ef64fa9aa2025-08-20T03:27:43ZengMDPI AGSeeds2674-10242025-06-01422710.3390/seeds4020027Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR)Stefania Mazzini0Marco Zuccolo1Angela Bassoli2Claudio Gardana3Gigliola Borgonovo4Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Agricultural and Environmental Sciences-Production, Landscape and Agroenergy-DiSAA, Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, ItalyDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, ItalySolid-state <sup>13</sup>C NMR spectroscopy using cross-polarization magic-angle spinning is a highly valuable technique for the semi-quantitative analysis of complex solid matrices. One of its key advantages is that it does not require any manipulation of the matrix, such as extractions or other treatments, which is particularly important for preserving the integrity of unstable secondary metabolites. Glucosinolates (β-thioglucoside-N-hydrosulphates) are crucial secondary metabolites specific to <i>Brassica</i> species, and many of them are known to be highly unstable. In this study, we evaluated solid-state nuclear magnetic resonance spectroscopy as an alternative method for the identification and quantification of total glucosinolates in the seeds of <i>Sisymbrium officinale</i>, <i>Brassica napus</i>, <i>Sinapis alba</i>, <i>Brassica nigra</i>, and <i>Moringa oleifera</i>. The results obtained with this method showed good agreement with those from conventional chemical analyses of the seed material. Although, based on a limited number of samples, this preliminary study suggests that the proposed approach could be a useful alternative for quantifying total glucosinolate content in seeds.https://www.mdpi.com/2674-1024/4/2/27glucosinolatessolid-state nuclear magnetic resonance (NMR)cross-polarization magic-angle spinning (CP-MAS)seedsfood plants |
| spellingShingle | Stefania Mazzini Marco Zuccolo Angela Bassoli Claudio Gardana Gigliola Borgonovo Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) Seeds glucosinolates solid-state nuclear magnetic resonance (NMR) cross-polarization magic-angle spinning (CP-MAS) seeds food plants |
| title | Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) |
| title_full | Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) |
| title_fullStr | Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) |
| title_full_unstemmed | Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) |
| title_short | Quantification of Glucosinolates in Seeds by Solid-State <sup>13</sup>C-Nuclear Magnetic Resonance (NMR) |
| title_sort | quantification of glucosinolates in seeds by solid state sup 13 sup c nuclear magnetic resonance nmr |
| topic | glucosinolates solid-state nuclear magnetic resonance (NMR) cross-polarization magic-angle spinning (CP-MAS) seeds food plants |
| url | https://www.mdpi.com/2674-1024/4/2/27 |
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