NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development
Abstract Background Nicotinamide phosphoribosyltransferase (NAMPT) is an enzyme that involves into NMN-NAD + synthesis which involves into cellular metabolism related with aging, immune function, and neurodegeneration. However, its roles in early embryo development are still unclear. Methods In pres...
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BMC
2025-05-01
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| Series: | Biological Research |
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| Online Access: | https://doi.org/10.1186/s40659-025-00608-3 |
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| author | Mei-Hua Liao Xin Liu Xiao-Ting Yu Shun Zhang You-Zhu Li Lin-Lin Hu Shao-Chen Sun Jun-Li Wang |
| author_facet | Mei-Hua Liao Xin Liu Xiao-Ting Yu Shun Zhang You-Zhu Li Lin-Lin Hu Shao-Chen Sun Jun-Li Wang |
| author_sort | Mei-Hua Liao |
| collection | DOAJ |
| description | Abstract Background Nicotinamide phosphoribosyltransferase (NAMPT) is an enzyme that involves into NMN-NAD + synthesis which involves into cellular metabolism related with aging, immune function, and neurodegeneration. However, its roles in early embryo development are still unclear. Methods In present study we disturbed the NAMPT activity and employed immunofluorescence staining and live cell imaging to explore its roles during early embryo development. Results We showed that NAMPT mRNA level was stable during mouse early embryo development, and NAMPT accumulated in the nucleus of blastomeres in mouse embryos. The loss of NAMPT activity disturbed the early cleavage from zygote to 2-cell, 4-cell to morula formation in the dose-dependent manner. We found that NAMPT inhibition disrupted mitochondria function in 2-cell embryos, showing decreased mitochondria number and aberrant accumulation in the blastomeres, which further disturb mitochondrial membrane potential level and elevated ROS level in embryos, indicating the occurrence of oxidative stress. Moreover, NAMPT inhibition also increased the apoptotic index, showing with increased Annexin-V signals and apoptotic gene expression. Conclusions Taken together, our study provided the evidence that NAMPT was essential for the mitochondria function to control oxidative stress and apoptosis during mouse early embryo development. |
| format | Article |
| id | doaj-art-c0b8eea6cff44d1e86d506dfd26fe308 |
| institution | OA Journals |
| issn | 0717-6287 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj-art-c0b8eea6cff44d1e86d506dfd26fe3082025-08-20T01:49:46ZengBMCBiological Research0717-62872025-05-0158111010.1186/s40659-025-00608-3NAMPT regulates mitochondria and oxidative stress level for mouse early embryo developmentMei-Hua Liao0Xin Liu1Xiao-Ting Yu2Shun Zhang3You-Zhu Li4Lin-Lin Hu5Shao-Chen Sun6Jun-Li Wang7Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for NationalitiesCollege of Animal Science and Technology, Nanjing Agricultural UniversityKey Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for NationalitiesDepartment of Reproductive Medical Center, The Affiliated Hospital of Guilin Medical UniversityDepartment of Reproductive Medicine, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen UniversityKey Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for NationalitiesKey Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for NationalitiesKey Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for NationalitiesAbstract Background Nicotinamide phosphoribosyltransferase (NAMPT) is an enzyme that involves into NMN-NAD + synthesis which involves into cellular metabolism related with aging, immune function, and neurodegeneration. However, its roles in early embryo development are still unclear. Methods In present study we disturbed the NAMPT activity and employed immunofluorescence staining and live cell imaging to explore its roles during early embryo development. Results We showed that NAMPT mRNA level was stable during mouse early embryo development, and NAMPT accumulated in the nucleus of blastomeres in mouse embryos. The loss of NAMPT activity disturbed the early cleavage from zygote to 2-cell, 4-cell to morula formation in the dose-dependent manner. We found that NAMPT inhibition disrupted mitochondria function in 2-cell embryos, showing decreased mitochondria number and aberrant accumulation in the blastomeres, which further disturb mitochondrial membrane potential level and elevated ROS level in embryos, indicating the occurrence of oxidative stress. Moreover, NAMPT inhibition also increased the apoptotic index, showing with increased Annexin-V signals and apoptotic gene expression. Conclusions Taken together, our study provided the evidence that NAMPT was essential for the mitochondria function to control oxidative stress and apoptosis during mouse early embryo development.https://doi.org/10.1186/s40659-025-00608-3OocyteActinMitochondriaOxidative stressParkinson’s disease |
| spellingShingle | Mei-Hua Liao Xin Liu Xiao-Ting Yu Shun Zhang You-Zhu Li Lin-Lin Hu Shao-Chen Sun Jun-Li Wang NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development Biological Research Oocyte Actin Mitochondria Oxidative stress Parkinson’s disease |
| title | NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development |
| title_full | NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development |
| title_fullStr | NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development |
| title_full_unstemmed | NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development |
| title_short | NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development |
| title_sort | nampt regulates mitochondria and oxidative stress level for mouse early embryo development |
| topic | Oocyte Actin Mitochondria Oxidative stress Parkinson’s disease |
| url | https://doi.org/10.1186/s40659-025-00608-3 |
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