Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic
The symbiotic matching for nodulation of Bradyrhizobium japonicum strains is a synergy of multi-proteins and plays a key role in symbiotic nitrogen fixation in nature. Studies on mechanism of symbiotic matching are significant in both theory and practice. In this paper, B. japonicum USDA110-A with h...
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KeAi Communications Co., Ltd.
2012-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311912601361 |
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| author | Da-wei GUAN Ming-chao MA Zhong-yu MA Xin JIANG Li LI Feng-ming CAO De-long SHEN Hui-jun CHEN Jun LI |
| author_facet | Da-wei GUAN Ming-chao MA Zhong-yu MA Xin JIANG Li LI Feng-ming CAO De-long SHEN Hui-jun CHEN Jun LI |
| author_sort | Da-wei GUAN |
| collection | DOAJ |
| description | The symbiotic matching for nodulation of Bradyrhizobium japonicum strains is a synergy of multi-proteins and plays a key role in symbiotic nitrogen fixation in nature. Studies on mechanism of symbiotic matching are significant in both theory and practice. In this paper, B. japonicum USDA110-A with high symbiotic matching with high-oil content soybean cultivar Suinong 20 and B. japonicum 2178 with low symbiotic matching were selected for proteomic to reveal mechanism of different symbiotic nodulation. The results showed that the amount and categories of proteins identified in this test were different when the two strains were treated by symbiotic nodulation. There were 10 up-regulated proteins and 5 down-regulated proteins with significant difference for B. japonicum USDA110-A. Proteins associated with nodulation and metabolism of energy and material, which were propitious to symbiotic nodulation, were all up-regulated, such as PHDPS synthase, metal-dependent phosphohydrolase, glycosyl transferase family. In contrast, only 5 up-regulated and 7 down-regulated differential proteins were detected in B. japonicum 2178. Molecular chaperones and defensive proteins, which influence the folding of nascent polypeptide chains and the active of azotase were down-regulated. To a certain extent, the different responses of B. japonicum to daidzein were one of the most important reasons that cause varieties in symbiotic matching ability. |
| format | Article |
| id | doaj-art-c0a8e55f34fa4bd08c70c1860a902127 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2012-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-c0a8e55f34fa4bd08c70c1860a9021272025-08-20T03:57:39ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192012-08-011181377138310.1016/S2095-3119(12)60136-1Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary ProteomicDa-wei GUAN0Ming-chao MA1Zhong-yu MA2Xin JIANG3Li LI4Feng-ming CAO5De-long SHEN6Hui-jun CHEN7Jun LI8Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. China; Key Laboratory of Plant Nutrition and Nutrient Cycling, Ministry of Agriculture, Beijing 100081, P.R. China; GUAN Da-wei, Tel/Fax: +86-10-82108675Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. China; Key Laboratory of Plant Nutrition and Nutrient Cycling, Ministry of Agriculture, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agriculture Sciences, Beijing 100081, P.R. China; Key Laboratory of Plant Nutrition and Nutrient Cycling, Ministry of Agriculture, Beijing 100081, P.R. China; Correspondence LI Jun, Tel/Fax: +86-10-82106208The symbiotic matching for nodulation of Bradyrhizobium japonicum strains is a synergy of multi-proteins and plays a key role in symbiotic nitrogen fixation in nature. Studies on mechanism of symbiotic matching are significant in both theory and practice. In this paper, B. japonicum USDA110-A with high symbiotic matching with high-oil content soybean cultivar Suinong 20 and B. japonicum 2178 with low symbiotic matching were selected for proteomic to reveal mechanism of different symbiotic nodulation. The results showed that the amount and categories of proteins identified in this test were different when the two strains were treated by symbiotic nodulation. There were 10 up-regulated proteins and 5 down-regulated proteins with significant difference for B. japonicum USDA110-A. Proteins associated with nodulation and metabolism of energy and material, which were propitious to symbiotic nodulation, were all up-regulated, such as PHDPS synthase, metal-dependent phosphohydrolase, glycosyl transferase family. In contrast, only 5 up-regulated and 7 down-regulated differential proteins were detected in B. japonicum 2178. Molecular chaperones and defensive proteins, which influence the folding of nascent polypeptide chains and the active of azotase were down-regulated. To a certain extent, the different responses of B. japonicum to daidzein were one of the most important reasons that cause varieties in symbiotic matching ability.http://www.sciencedirect.com/science/article/pii/S2095311912601361Bradyrhizobium japonicumdaidzeinsymbiotic matching for nodulationproteomic technique |
| spellingShingle | Da-wei GUAN Ming-chao MA Zhong-yu MA Xin JIANG Li LI Feng-ming CAO De-long SHEN Hui-jun CHEN Jun LI Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic Journal of Integrative Agriculture Bradyrhizobium japonicum daidzein symbiotic matching for nodulation proteomic technique |
| title | Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic |
| title_full | Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic |
| title_fullStr | Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic |
| title_full_unstemmed | Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic |
| title_short | Analysis of Two Bradyrhizobium japonicum Strains with Different Symbiotic Matching for Nodulation by Primary Proteomic |
| title_sort | analysis of two bradyrhizobium japonicum strains with different symbiotic matching for nodulation by primary proteomic |
| topic | Bradyrhizobium japonicum daidzein symbiotic matching for nodulation proteomic technique |
| url | http://www.sciencedirect.com/science/article/pii/S2095311912601361 |
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