The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes
This study was designed to identify the efficiency of interferon-stimulated gene 15 (ISG15) expression for early pregnancy detection in Awassi ewes. A total of 10 ewes were enrolled: 3 carrying twins, 4 carrying single pregnancies, and 3 non-pregnant. Blood samples were collected on 0, 6, 15, and 25...
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| Format: | Article |
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_186171_ac20c1dde49ed8c074992ee3d70b9de9.pdf |
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| author | Eman H. Lazim Khawla A. Hussein Dhafer M. Aziz |
| author_facet | Eman H. Lazim Khawla A. Hussein Dhafer M. Aziz |
| author_sort | Eman H. Lazim |
| collection | DOAJ |
| description | This study was designed to identify the efficiency of interferon-stimulated gene 15 (ISG15) expression for early pregnancy detection in Awassi ewes. A total of 10 ewes were enrolled: 3 carrying twins, 4 carrying single pregnancies, and 3 non-pregnant. Blood samples were collected on 0, 6, 15, and 25 days of gestation to measure ISG15 expression levels using quantitative real-time PCR (qRT-PCR). The results of the relative expression values of ISG15 in pregnant and non-pregnant ewes on 0, 6, 15, and 25 days of pregnancy were 0.044±0.012 vs. 0.049±0.009, 0.203±0.083 vs. 0.201±0.093, 1.839±0.594 vs. 0.292±0.136, and 0.583±0.067 vs 0.200±0.051, respectively. In both twin and single pregnant ewes, the relative expression values of ISG15 were increased significantly (P<0.05) on 15 day of pregnancy, after that the values declined significantly (P<0.05) on day 25. No significant differences in ISG15 expression were observed between ewes carrying twins and those carrying single fetuses. Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) on days 0 and 6 of pregnancy was 0.405, whereas on days 15 and 25 it was 0.952 and 1.00, respectively. The accuracy, specificity, sensitivity, and positive and negative predictive values of ISG15 expression on days 15 and 25 of pregnancy were 90%, 100%, 85.7%, 100%, and 75%, and 100%, 100%, 100%, 100%, and 100%, respectively. In conclusion, ISG15 expression demonstrated high accuracy as an early pregnancy marker in Awassi ewes, with greater diagnostic reliability on day 25 compared to day 15 of gestation. |
| format | Article |
| id | doaj-art-c0a03d5b49fb4d178d781351543ad198 |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-c0a03d5b49fb4d178d781351543ad1982025-02-04T13:11:43ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139116917610.33899/ijvs.2025.155210.4021186171The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewesEman H. Lazim0Khawla A. Hussein1Dhafer M. Aziz2Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, IraqDepartment of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, IraqThis study was designed to identify the efficiency of interferon-stimulated gene 15 (ISG15) expression for early pregnancy detection in Awassi ewes. A total of 10 ewes were enrolled: 3 carrying twins, 4 carrying single pregnancies, and 3 non-pregnant. Blood samples were collected on 0, 6, 15, and 25 days of gestation to measure ISG15 expression levels using quantitative real-time PCR (qRT-PCR). The results of the relative expression values of ISG15 in pregnant and non-pregnant ewes on 0, 6, 15, and 25 days of pregnancy were 0.044±0.012 vs. 0.049±0.009, 0.203±0.083 vs. 0.201±0.093, 1.839±0.594 vs. 0.292±0.136, and 0.583±0.067 vs 0.200±0.051, respectively. In both twin and single pregnant ewes, the relative expression values of ISG15 were increased significantly (P<0.05) on 15 day of pregnancy, after that the values declined significantly (P<0.05) on day 25. No significant differences in ISG15 expression were observed between ewes carrying twins and those carrying single fetuses. Receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) on days 0 and 6 of pregnancy was 0.405, whereas on days 15 and 25 it was 0.952 and 1.00, respectively. The accuracy, specificity, sensitivity, and positive and negative predictive values of ISG15 expression on days 15 and 25 of pregnancy were 90%, 100%, 85.7%, 100%, and 75%, and 100%, 100%, 100%, 100%, and 100%, respectively. In conclusion, ISG15 expression demonstrated high accuracy as an early pregnancy marker in Awassi ewes, with greater diagnostic reliability on day 25 compared to day 15 of gestation.https://www.vetmedmosul.com/article_186171_ac20c1dde49ed8c074992ee3d70b9de9.pdfawassi ewesqrt-pcraccuracyisg15pregnancy |
| spellingShingle | Eman H. Lazim Khawla A. Hussein Dhafer M. Aziz The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes Iraqi Journal of Veterinary Sciences awassi ewes qrt-pcr accuracy isg15 pregnancy |
| title | The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes |
| title_full | The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes |
| title_fullStr | The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes |
| title_full_unstemmed | The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes |
| title_short | The efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in Awassi ewes |
| title_sort | efficiency of interferon stimulated gene 15 expression in peripheral blood for early pregnancy prediction in awassi ewes |
| topic | awassi ewes qrt-pcr accuracy isg15 pregnancy |
| url | https://www.vetmedmosul.com/article_186171_ac20c1dde49ed8c074992ee3d70b9de9.pdf |
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