Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>

<i>Penaeus semisulcatus</i>, a commercially valuable aquaculture species, is widely distributed along the southeastern coast of China and throughout the South China Sea. Despite its economic importance, the comprehensive understanding of its germplasm characteristics remains limited. Thi...

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Main Authors: Yundong Li, Juan Chen, Ziyi Jiang, Song Jiang, Qibin Yang, Lishi Yang, Jianhua Huang, Jianzhi Shi, Yangyang Ding, Tianmi Liu, Falin Zhou
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Journal of Marine Science and Engineering
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Online Access:https://www.mdpi.com/2077-1312/13/4/655
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author Yundong Li
Juan Chen
Ziyi Jiang
Song Jiang
Qibin Yang
Lishi Yang
Jianhua Huang
Jianzhi Shi
Yangyang Ding
Tianmi Liu
Falin Zhou
author_facet Yundong Li
Juan Chen
Ziyi Jiang
Song Jiang
Qibin Yang
Lishi Yang
Jianhua Huang
Jianzhi Shi
Yangyang Ding
Tianmi Liu
Falin Zhou
author_sort Yundong Li
collection DOAJ
description <i>Penaeus semisulcatus</i>, a commercially valuable aquaculture species, is widely distributed along the southeastern coast of China and throughout the South China Sea. Despite its economic importance, the comprehensive understanding of its germplasm characteristics remains limited. This investigation evaluated nutrient profiles, amino acid composition, lipid constituents, antioxidant capacity, and genomic variability across three farmed aquaculture populations maintained under standardized environmental parameters to discern divergence in nutritive attributes and hereditary characteristics. The results revealed significant interpopulation variation in ash content (<i>p</i> < 0.05), while other proximate components showed comparable levels. Among 17 detected amino acids, glutamic acid was the most abundant (2.07–2.30 g/100 g), while cysteine had the lowest concentration (0.10–0.11 g/100 g). Notably, the PsHK population had a relatively higher amino acid content, indicating superior nutritional value. Fatty acid analysis detected 13 fatty acids, with C18:0 (88.27–108.00 mg/100 g) being the most abundant, and C20:2 (3.40–4.70 mg/100 g) the least. The PsHK population exhibited significantly higher levels of all fatty acids compared to the other two populations (<i>p</i> < 0.05). Antioxidant enzyme activity assays revealed no significant differences in antioxidant capacity across the three populations, indicating a similar overall antioxidant status. Genetic diversity analysis indicated that the PsHK population had the highest genetic diversity, with a relatively pronounced genetic differentiation between the PsHK and PsRP populations. In conclusion, the comparative analysis of these three cultured populations highlights that the PsHK population excels in both nutritional composition and genetic diversity. With its superior nutritional profile and rich genetic background, <i>P. semisulcatus</i> shows great potential for aquaculture development. These findings provide valuable insights for future germplasm improvement and aquaculture optimization efforts, offering a scientific basis for refining breeding strategies and enhancing the nutritional evaluation of <i>P. semisulcatus</i>. Additionally, the comparative analysis of genetic diversity and biochemical composition contributes to a better understanding of population variation, which is essential for the sustainable management and utilization of this species in aquaculture.
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spelling doaj-art-c0969b5a37ff4391a7fc452bdc07c4d22025-08-20T02:28:31ZengMDPI AGJournal of Marine Science and Engineering2077-13122025-03-0113465510.3390/jmse13040655Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>Yundong Li0Juan Chen1Ziyi Jiang2Song Jiang3Qibin Yang4Lishi Yang5Jianhua Huang6Jianzhi Shi7Yangyang Ding8Tianmi Liu9Falin Zhou10South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaKeck School of Medicine, University of Southern California, 1975 Zonal Ave, Los Angeles, CA 90033, USASouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, ChinaFisheries Technology Extension Center of Hainan Province, Haikou 570311, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China<i>Penaeus semisulcatus</i>, a commercially valuable aquaculture species, is widely distributed along the southeastern coast of China and throughout the South China Sea. Despite its economic importance, the comprehensive understanding of its germplasm characteristics remains limited. This investigation evaluated nutrient profiles, amino acid composition, lipid constituents, antioxidant capacity, and genomic variability across three farmed aquaculture populations maintained under standardized environmental parameters to discern divergence in nutritive attributes and hereditary characteristics. The results revealed significant interpopulation variation in ash content (<i>p</i> < 0.05), while other proximate components showed comparable levels. Among 17 detected amino acids, glutamic acid was the most abundant (2.07–2.30 g/100 g), while cysteine had the lowest concentration (0.10–0.11 g/100 g). Notably, the PsHK population had a relatively higher amino acid content, indicating superior nutritional value. Fatty acid analysis detected 13 fatty acids, with C18:0 (88.27–108.00 mg/100 g) being the most abundant, and C20:2 (3.40–4.70 mg/100 g) the least. The PsHK population exhibited significantly higher levels of all fatty acids compared to the other two populations (<i>p</i> < 0.05). Antioxidant enzyme activity assays revealed no significant differences in antioxidant capacity across the three populations, indicating a similar overall antioxidant status. Genetic diversity analysis indicated that the PsHK population had the highest genetic diversity, with a relatively pronounced genetic differentiation between the PsHK and PsRP populations. In conclusion, the comparative analysis of these three cultured populations highlights that the PsHK population excels in both nutritional composition and genetic diversity. With its superior nutritional profile and rich genetic background, <i>P. semisulcatus</i> shows great potential for aquaculture development. These findings provide valuable insights for future germplasm improvement and aquaculture optimization efforts, offering a scientific basis for refining breeding strategies and enhancing the nutritional evaluation of <i>P. semisulcatus</i>. Additionally, the comparative analysis of genetic diversity and biochemical composition contributes to a better understanding of population variation, which is essential for the sustainable management and utilization of this species in aquaculture.https://www.mdpi.com/2077-1312/13/4/655<i>Penaeus semisulcatus</i>amino acidsfatty acidsantioxidant enzyme activitygenetic diversity
spellingShingle Yundong Li
Juan Chen
Ziyi Jiang
Song Jiang
Qibin Yang
Lishi Yang
Jianhua Huang
Jianzhi Shi
Yangyang Ding
Tianmi Liu
Falin Zhou
Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
Journal of Marine Science and Engineering
<i>Penaeus semisulcatus</i>
amino acids
fatty acids
antioxidant enzyme activity
genetic diversity
title Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
title_full Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
title_fullStr Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
title_full_unstemmed Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
title_short Comparative Analysis of Amino Acid Composition, Fatty Acid Profiles, and Genetic Diversity Among Three Populations of <i>Penaeus semisulcatus</i>
title_sort comparative analysis of amino acid composition fatty acid profiles and genetic diversity among three populations of i penaeus semisulcatus i
topic <i>Penaeus semisulcatus</i>
amino acids
fatty acids
antioxidant enzyme activity
genetic diversity
url https://www.mdpi.com/2077-1312/13/4/655
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