Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey
Ash shoestring-associated virus (ASaV; <i>Emaravirus fraxini</i>) is a five-segmented, negative-sense RNA virus associated with chlorosis and leaf deformation in <i>Fraxinus</i> species. This study investigated the genetic diversity and evolutionary dynamics of ASaV by analyz...
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MDPI AG
2025-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/3/633 |
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| author | Sahar Nouri Susanne von Bargen Artemis Rumbou Thomas R. Gaskin Carmen Büttner Shaheen Nourinejhad Zarghani |
| author_facet | Sahar Nouri Susanne von Bargen Artemis Rumbou Thomas R. Gaskin Carmen Büttner Shaheen Nourinejhad Zarghani |
| author_sort | Sahar Nouri |
| collection | DOAJ |
| description | Ash shoestring-associated virus (ASaV; <i>Emaravirus fraxini</i>) is a five-segmented, negative-sense RNA virus associated with chlorosis and leaf deformation in <i>Fraxinus</i> species. This study investigated the genetic diversity and evolutionary dynamics of ASaV by analyzing nearly full-length RNA2–RNA5 and partial RNA1 sequences from isolates collected from different geographic regions and <i>Fraxinus</i> hosts. The sequence data uncovered that ASaV has a conserved genome, in which RNA3 and RNA5 showed more genetic divergence than other segments in the sequenced isolates. ASaV RNA3 and partial RNA1 were the most informative genomic regions for phylogenetic studies. There was a correlation between the clustering of the ASaV isolates and host species when the phylogenetic tree was constructed based on the RNA1 region. The ASaV genome is predominantly under purifying selection. Newly designed primers in this study facilitated robust amplification of genomic regions. |
| format | Article |
| id | doaj-art-c08a62a8520341aeb04571bc6a59f8df |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c08a62a8520341aeb04571bc6a59f8df2025-08-20T01:49:05ZengMDPI AGMicroorganisms2076-26072025-03-0113363310.3390/microorganisms13030633Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide SurveySahar Nouri0Susanne von Bargen1Artemis Rumbou2Thomas R. Gaskin3Carmen Büttner4Shaheen Nourinejhad Zarghani5Division of Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Lentzeallee 55–57, 14195 Berlin, GermanyDivision of Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Lentzeallee 55–57, 14195 Berlin, GermanyDivision of Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Lentzeallee 55–57, 14195 Berlin, GermanyBrandenburg State Office of Rural Development, Agriculture and Land Consolidation, Müllroser Chaussee 54, 15236 Frankfurt (Oder), GermanyDivision of Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Lentzeallee 55–57, 14195 Berlin, GermanyDivision of Phytomedicine, Faculty of Life Sciences, Albrecht Daniel Thaer-Institute of Agricultural and Horticultural Sciences, Humboldt University of Berlin, Lentzeallee 55–57, 14195 Berlin, GermanyAsh shoestring-associated virus (ASaV; <i>Emaravirus fraxini</i>) is a five-segmented, negative-sense RNA virus associated with chlorosis and leaf deformation in <i>Fraxinus</i> species. This study investigated the genetic diversity and evolutionary dynamics of ASaV by analyzing nearly full-length RNA2–RNA5 and partial RNA1 sequences from isolates collected from different geographic regions and <i>Fraxinus</i> hosts. The sequence data uncovered that ASaV has a conserved genome, in which RNA3 and RNA5 showed more genetic divergence than other segments in the sequenced isolates. ASaV RNA3 and partial RNA1 were the most informative genomic regions for phylogenetic studies. There was a correlation between the clustering of the ASaV isolates and host species when the phylogenetic tree was constructed based on the RNA1 region. The ASaV genome is predominantly under purifying selection. Newly designed primers in this study facilitated robust amplification of genomic regions.https://www.mdpi.com/2076-2607/13/3/633emaravirusforestnegative-strand RNA virusevolutionpopulation parametersgenetic differentiation |
| spellingShingle | Sahar Nouri Susanne von Bargen Artemis Rumbou Thomas R. Gaskin Carmen Büttner Shaheen Nourinejhad Zarghani Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey Microorganisms emaravirus forest negative-strand RNA virus evolution population parameters genetic differentiation |
| title | Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey |
| title_full | Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey |
| title_fullStr | Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey |
| title_full_unstemmed | Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey |
| title_short | Evolutionary Dynamics and Population Genetics of Ash Shoestring-Associated Virus in a European-Wide Survey |
| title_sort | evolutionary dynamics and population genetics of ash shoestring associated virus in a european wide survey |
| topic | emaravirus forest negative-strand RNA virus evolution population parameters genetic differentiation |
| url | https://www.mdpi.com/2076-2607/13/3/633 |
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