Verteporfin is an effective inhibitor of HCMV replication
Human cytomegalovirus (HCMV), a double-stranded DNA virus from the Betaherpesvirinae subfamily, constitutes significant risks to newborns and immunocompromised individuals, potentially leading to severe neurodevelopmental disorders. The purpose of this study was to identify FDA-approved drugs that c...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170224001680 |
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| author | Woo Young Lim Ju Hyun Lee Youngju Choi Keejung Yoon |
| author_facet | Woo Young Lim Ju Hyun Lee Youngju Choi Keejung Yoon |
| author_sort | Woo Young Lim |
| collection | DOAJ |
| description | Human cytomegalovirus (HCMV), a double-stranded DNA virus from the Betaherpesvirinae subfamily, constitutes significant risks to newborns and immunocompromised individuals, potentially leading to severe neurodevelopmental disorders. The purpose of this study was to identify FDA-approved drugs that can inhibit HCMV replication through a drug repositioning approach. Using an HCMV progeny assay, verteporfin, a medication used as a photosensitizer in photodynamic therapy, was found to inhibit HCMV production in a dose-dependent manner, significantly reducing replication at concentrations as low as 0.5 µM, approximately 1/20th of the concentration used in anti-cancer research. Further analysis revealed that verteporfin did not interfere with HCMV host cell entry or nuclear transport but reduced viral mRNA and protein levels throughout the HCMV life cycle from the immediate-early stages. These results suggest that verteporfin has the potential to be rapidly and safely developed as a repurposed drug to inhibit HCMV infection. |
| format | Article |
| id | doaj-art-c089acf674b64df883c2db19cedf0b67 |
| institution | Kabale University |
| issn | 1872-7492 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-c089acf674b64df883c2db19cedf0b672024-12-19T10:52:16ZengElsevierVirus Research1872-74922024-12-01350199475Verteporfin is an effective inhibitor of HCMV replicationWoo Young Lim0Ju Hyun Lee1Youngju Choi2Keejung Yoon3Department of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, South KoreaDepartment of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, South KoreaDepartment of Microbiology, School of Medicine, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, South KoreaDepartment of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, South Korea; College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, South Korea; Corresponding author.Human cytomegalovirus (HCMV), a double-stranded DNA virus from the Betaherpesvirinae subfamily, constitutes significant risks to newborns and immunocompromised individuals, potentially leading to severe neurodevelopmental disorders. The purpose of this study was to identify FDA-approved drugs that can inhibit HCMV replication through a drug repositioning approach. Using an HCMV progeny assay, verteporfin, a medication used as a photosensitizer in photodynamic therapy, was found to inhibit HCMV production in a dose-dependent manner, significantly reducing replication at concentrations as low as 0.5 µM, approximately 1/20th of the concentration used in anti-cancer research. Further analysis revealed that verteporfin did not interfere with HCMV host cell entry or nuclear transport but reduced viral mRNA and protein levels throughout the HCMV life cycle from the immediate-early stages. These results suggest that verteporfin has the potential to be rapidly and safely developed as a repurposed drug to inhibit HCMV infection.http://www.sciencedirect.com/science/article/pii/S0168170224001680Human cytomegalovirusVerteporfinDrug repositioningAntiviral activity |
| spellingShingle | Woo Young Lim Ju Hyun Lee Youngju Choi Keejung Yoon Verteporfin is an effective inhibitor of HCMV replication Virus Research Human cytomegalovirus Verteporfin Drug repositioning Antiviral activity |
| title | Verteporfin is an effective inhibitor of HCMV replication |
| title_full | Verteporfin is an effective inhibitor of HCMV replication |
| title_fullStr | Verteporfin is an effective inhibitor of HCMV replication |
| title_full_unstemmed | Verteporfin is an effective inhibitor of HCMV replication |
| title_short | Verteporfin is an effective inhibitor of HCMV replication |
| title_sort | verteporfin is an effective inhibitor of hcmv replication |
| topic | Human cytomegalovirus Verteporfin Drug repositioning Antiviral activity |
| url | http://www.sciencedirect.com/science/article/pii/S0168170224001680 |
| work_keys_str_mv | AT wooyounglim verteporfinisaneffectiveinhibitorofhcmvreplication AT juhyunlee verteporfinisaneffectiveinhibitorofhcmvreplication AT youngjuchoi verteporfinisaneffectiveinhibitorofhcmvreplication AT keejungyoon verteporfinisaneffectiveinhibitorofhcmvreplication |