NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.
Chromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes...
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| Language: | English |
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Public Library of Science (PLoS)
2021-07-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1009459&type=printable |
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| author | Xue Cheng Valérie Côté Jacques Côté |
| author_facet | Xue Cheng Valérie Côté Jacques Côté |
| author_sort | Xue Cheng |
| collection | DOAJ |
| description | Chromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes upon DSB induction and defects in its activity correlate with altered DNA end resection and Rad51 recombinase recruitment. Importantly, we show that NuA4 is also recruited to the donor sequence during recombination along with increased H4 acetylation, indicating a direct role during strand invasion/D-loop formation after resection. We found that NuA4 cooperates locally with another HAT, the SAGA complex, during DSB repair as their combined action is essential for DNA end resection to occur. This cooperation of NuA4 and SAGA is required for recruitment of ATP-dependent chromatin remodelers, targeted acetylation of repair factors and homologous recombination. Our work reveals a multifaceted and conserved cooperation mechanism between acetyltransferase complexes to allow repair of DNA breaks by homologous recombination. |
| format | Article |
| id | doaj-art-c07c9a3c9fda49bf89c41828cf83325a |
| institution | DOAJ |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-c07c9a3c9fda49bf89c41828cf83325a2025-08-20T02:55:32ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-07-01177e100945910.1371/journal.pgen.1009459NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination.Xue ChengValérie CôtéJacques CôtéChromatin modifying complexes play important yet not fully defined roles in DNA repair processes. The essential NuA4 histone acetyltransferase (HAT) complex is recruited to double-strand break (DSB) sites and spreads along with DNA end resection. As predicted, NuA4 acetylates surrounding nucleosomes upon DSB induction and defects in its activity correlate with altered DNA end resection and Rad51 recombinase recruitment. Importantly, we show that NuA4 is also recruited to the donor sequence during recombination along with increased H4 acetylation, indicating a direct role during strand invasion/D-loop formation after resection. We found that NuA4 cooperates locally with another HAT, the SAGA complex, during DSB repair as their combined action is essential for DNA end resection to occur. This cooperation of NuA4 and SAGA is required for recruitment of ATP-dependent chromatin remodelers, targeted acetylation of repair factors and homologous recombination. Our work reveals a multifaceted and conserved cooperation mechanism between acetyltransferase complexes to allow repair of DNA breaks by homologous recombination.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1009459&type=printable |
| spellingShingle | Xue Cheng Valérie Côté Jacques Côté NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. PLoS Genetics |
| title | NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. |
| title_full | NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. |
| title_fullStr | NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. |
| title_full_unstemmed | NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. |
| title_short | NuA4 and SAGA acetyltransferase complexes cooperate for repair of DNA breaks by homologous recombination. |
| title_sort | nua4 and saga acetyltransferase complexes cooperate for repair of dna breaks by homologous recombination |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1009459&type=printable |
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