Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation
PAH contamination from coking plants have received widespread attention. However, the microbial diversity, co-occurrence patterns, and functional genes of bacteria in aged coking contaminated soils by PAHs are still not clear. In our study, we used a macro-genetic approach to detect PAH-contaminated...
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2025-04-01
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| author | Liping Zheng Yifan Yan Qun Li Junyang Du Xiaosong Lu Li Xu Qunhui Xie Yangsheng Chen Aiguo Zhang Bin Zhao |
| author_facet | Liping Zheng Yifan Yan Qun Li Junyang Du Xiaosong Lu Li Xu Qunhui Xie Yangsheng Chen Aiguo Zhang Bin Zhao |
| author_sort | Liping Zheng |
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| description | PAH contamination from coking plants have received widespread attention. However, the microbial diversity, co-occurrence patterns, and functional genes of bacteria in aged coking contaminated soils by PAHs are still not clear. In our study, we used a macro-genetic approach to detect PAH-contaminated soils from both a coking production area (CA group) and an office zone (OA group) in an abandoned coking plant, and analyzed the characteristic bacteria and function genes, microbial network interaction patterns, and soil P-cycling in long-term PAH-contaminated soils. The results revealed that <i>Proteobacteria</i> were significantly positively correlated with PAHs and <i>Betaprobacteria bacterium</i> rifcsplowo2 12 full 6514, candidatus <i>Muproteobacteria bacterium</i> RBG16609, and <i>Sulfurifustis variabilis</i>, which belong to <i>Proteobacteria</i>, were characteristic bacteria in PAH-contaminated soils. The phn, which is the PAH degradation gene, was abundantly expressed in the PAH-contaminated soil. The <i>phn</i> gene cluster genes (<i>phnE</i>, <i>phnC</i>, and <i>phnD</i>) were significantly expressed in the CA group of PAH-contaminated soils (<i>p</i> < 0.05). By integrating microbial diversity, network structure, and functional genes, it offers a comprehensive understanding of soil ecosystem response indicators to prolonged PAH stress. The results of this study will provide new ideas for constructing an assessment index system for soil health and screening biomarkers for PAH-contaminated soils. |
| format | Article |
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| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Microorganisms |
| spelling | doaj-art-c07c5340a38c405db536ca62c06ee03e2025-08-20T02:28:18ZengMDPI AGMicroorganisms2076-26072025-04-0113486910.3390/microorganisms13040869Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and BioremediationLiping Zheng0Yifan Yan1Qun Li2Junyang Du3Xiaosong Lu4Li Xu5Qunhui Xie6Yangsheng Chen7Aiguo Zhang8Bin Zhao9State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, ChinaState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, ChinaState Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, ChinaSchool of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, ChinaSchool of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 310024, ChinaPAH contamination from coking plants have received widespread attention. However, the microbial diversity, co-occurrence patterns, and functional genes of bacteria in aged coking contaminated soils by PAHs are still not clear. In our study, we used a macro-genetic approach to detect PAH-contaminated soils from both a coking production area (CA group) and an office zone (OA group) in an abandoned coking plant, and analyzed the characteristic bacteria and function genes, microbial network interaction patterns, and soil P-cycling in long-term PAH-contaminated soils. The results revealed that <i>Proteobacteria</i> were significantly positively correlated with PAHs and <i>Betaprobacteria bacterium</i> rifcsplowo2 12 full 6514, candidatus <i>Muproteobacteria bacterium</i> RBG16609, and <i>Sulfurifustis variabilis</i>, which belong to <i>Proteobacteria</i>, were characteristic bacteria in PAH-contaminated soils. The phn, which is the PAH degradation gene, was abundantly expressed in the PAH-contaminated soil. The <i>phn</i> gene cluster genes (<i>phnE</i>, <i>phnC</i>, and <i>phnD</i>) were significantly expressed in the CA group of PAH-contaminated soils (<i>p</i> < 0.05). By integrating microbial diversity, network structure, and functional genes, it offers a comprehensive understanding of soil ecosystem response indicators to prolonged PAH stress. The results of this study will provide new ideas for constructing an assessment index system for soil health and screening biomarkers for PAH-contaminated soils.https://www.mdpi.com/2076-2607/13/4/869PAHscoking plantmicrobial diversityco-occurrence patternsfunctional gene |
| spellingShingle | Liping Zheng Yifan Yan Qun Li Junyang Du Xiaosong Lu Li Xu Qunhui Xie Yangsheng Chen Aiguo Zhang Bin Zhao Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation Microorganisms PAHs coking plant microbial diversity co-occurrence patterns functional gene |
| title | Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation |
| title_full | Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation |
| title_fullStr | Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation |
| title_full_unstemmed | Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation |
| title_short | Microbial Diversity, Co-Occurrence Patterns, and Functional Genes of Bacteria in Aged Coking Contaminated Soils by Polycyclic Aromatic Hydrocarbons: Implications to Soil Health and Bioremediation |
| title_sort | microbial diversity co occurrence patterns and functional genes of bacteria in aged coking contaminated soils by polycyclic aromatic hydrocarbons implications to soil health and bioremediation |
| topic | PAHs coking plant microbial diversity co-occurrence patterns functional gene |
| url | https://www.mdpi.com/2076-2607/13/4/869 |
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