Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat

We investigated whether maternal diabetes induced in rats using streptozotocin (STZ) on Day 5 of pregnancy affects the intrauterine developmental timeline. A total of 30 pregnant Sprague-Dawley diabetic rats (DRs) and 20 control rats (CRs) were used to obtain 21-day fetuses (F21) and newborn (NB) pu...

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Main Authors: Marcela Salazar García, Elba Reyes Maldonado, María Cristina Revilla Monsalve, Laura Villavicencio Guzmán, Alfonso Reyes López, Concepción Sánchez-Gómez
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Diabetes Research
Online Access:http://dx.doi.org/10.1155/2015/354265
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author Marcela Salazar García
Elba Reyes Maldonado
María Cristina Revilla Monsalve
Laura Villavicencio Guzmán
Alfonso Reyes López
Concepción Sánchez-Gómez
author_facet Marcela Salazar García
Elba Reyes Maldonado
María Cristina Revilla Monsalve
Laura Villavicencio Guzmán
Alfonso Reyes López
Concepción Sánchez-Gómez
author_sort Marcela Salazar García
collection DOAJ
description We investigated whether maternal diabetes induced in rats using streptozotocin (STZ) on Day 5 of pregnancy affects the intrauterine developmental timeline. A total of 30 pregnant Sprague-Dawley diabetic rats (DRs) and 20 control rats (CRs) were used to obtain 21-day fetuses (F21) and newborn (NB) pups. Gestational age, weight, and body size were recorded as were the maxillofacial morphometry and morphohistological characteristics of the limbs. In DRs, pregnancy continued for ∼1.7 days, and delivery occurred 23 days postcoitus (DPC). In this group, the number of pups was lower, and 13% had maxillofacial defects. F21 in the DR group had lower weights and were smaller; moreover, the morphological characteristics of the maxillofacial structures, derived from the neural crest, were discordant with their chronological gestational age, resembling 18- to 19-day-old fetuses. These deficiencies were counterbalanced in NB pups. We conclude that hyperglycemia, which results from maternal diabetes and precedes embryo implantation, deregulates the intrauterine developmental timeline, restricts embryo-fetal growth, and primarily delays the remodeling and maturation of the structures derived from neural crest cells.
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spelling doaj-art-c079e2e591f04350848ac4dfc2041ea82025-08-20T02:19:23ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/354265354265Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in RatMarcela Salazar García0Elba Reyes Maldonado1María Cristina Revilla Monsalve2Laura Villavicencio Guzmán3Alfonso Reyes López4Concepción Sánchez-Gómez5Laboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Colonia Doctores, DF, MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala, 11340 Colonia Santo Tomas, DF, MexicoUnidad de Investigación Médica en Enfermedades Metabólicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Avenida Cuauhtémoc 330, 06725 Colonia Doctores, DF, MexicoLaboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Colonia Doctores, DF, MexicoDirección de Investigación, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Colonia Doctores, DF, MexicoLaboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, 06720 Colonia Doctores, DF, MexicoWe investigated whether maternal diabetes induced in rats using streptozotocin (STZ) on Day 5 of pregnancy affects the intrauterine developmental timeline. A total of 30 pregnant Sprague-Dawley diabetic rats (DRs) and 20 control rats (CRs) were used to obtain 21-day fetuses (F21) and newborn (NB) pups. Gestational age, weight, and body size were recorded as were the maxillofacial morphometry and morphohistological characteristics of the limbs. In DRs, pregnancy continued for ∼1.7 days, and delivery occurred 23 days postcoitus (DPC). In this group, the number of pups was lower, and 13% had maxillofacial defects. F21 in the DR group had lower weights and were smaller; moreover, the morphological characteristics of the maxillofacial structures, derived from the neural crest, were discordant with their chronological gestational age, resembling 18- to 19-day-old fetuses. These deficiencies were counterbalanced in NB pups. We conclude that hyperglycemia, which results from maternal diabetes and precedes embryo implantation, deregulates the intrauterine developmental timeline, restricts embryo-fetal growth, and primarily delays the remodeling and maturation of the structures derived from neural crest cells.http://dx.doi.org/10.1155/2015/354265
spellingShingle Marcela Salazar García
Elba Reyes Maldonado
María Cristina Revilla Monsalve
Laura Villavicencio Guzmán
Alfonso Reyes López
Concepción Sánchez-Gómez
Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
Journal of Diabetes Research
title Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
title_full Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
title_fullStr Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
title_full_unstemmed Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
title_short Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat
title_sort importance of maternal diabetes on the chronological deregulation of the intrauterine development an experimental study in rat
url http://dx.doi.org/10.1155/2015/354265
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