A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b.
MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0178045&type=printable |
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| _version_ | 1841533119768821760 |
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| author | Wenyang Sun Jing Lan Lei Chen Jinjie Qiu Zonggang Luo Mingzhou Li Jinyong Wang Jiugang Zhao Tinghuan Zhang Xi Long Jie Chai Zunqiang Yan Zongyi Guo Shuangbao Gun |
| author_facet | Wenyang Sun Jing Lan Lei Chen Jinjie Qiu Zonggang Luo Mingzhou Li Jinyong Wang Jiugang Zhao Tinghuan Zhang Xi Long Jie Chai Zunqiang Yan Zongyi Guo Shuangbao Gun |
| author_sort | Wenyang Sun |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA biogenesis. |
| format | Article |
| id | doaj-art-c076864cb3e5497c91f33913a9690a94 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c076864cb3e5497c91f33913a9690a942025-01-17T05:32:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01125e017804510.1371/journal.pone.0178045A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b.Wenyang SunJing LanLei ChenJinjie QiuZonggang LuoMingzhou LiJinyong WangJiugang ZhaoTinghuan ZhangXi LongJie ChaiZunqiang YanZongyi GuoShuangbao GunMicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA biogenesis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0178045&type=printable |
| spellingShingle | Wenyang Sun Jing Lan Lei Chen Jinjie Qiu Zonggang Luo Mingzhou Li Jinyong Wang Jiugang Zhao Tinghuan Zhang Xi Long Jie Chai Zunqiang Yan Zongyi Guo Shuangbao Gun A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. PLoS ONE |
| title | A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. |
| title_full | A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. |
| title_fullStr | A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. |
| title_full_unstemmed | A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. |
| title_short | A mutation in porcine pre-miR-15b alters the biogenesis of MiR-15b\16-1 cluster and strand selection of MiR-15b. |
| title_sort | mutation in porcine pre mir 15b alters the biogenesis of mir 15b 16 1 cluster and strand selection of mir 15b |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0178045&type=printable |
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