Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma
Background. Innate immune antimicrobial peptides, including β-defensin-1, promote the chemotaxis and activation of several immune cells. The role of β-defensin-1 in asthma and chronic obstructive pulmonary disease (COPD) remains unclear. Methods. Induced sputum was collected from healthy controls an...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/407271 |
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| author | Katherine J. Baines Thomas K. Wright Jodie L. Simpson Vanessa M. McDonald Lisa G. Wood Kristy S. Parsons Peter A. Wark Peter G. Gibson |
| author_facet | Katherine J. Baines Thomas K. Wright Jodie L. Simpson Vanessa M. McDonald Lisa G. Wood Kristy S. Parsons Peter A. Wark Peter G. Gibson |
| author_sort | Katherine J. Baines |
| collection | DOAJ |
| description | Background. Innate immune antimicrobial peptides, including β-defensin-1, promote the chemotaxis and activation of several immune cells. The role of β-defensin-1 in asthma and chronic obstructive pulmonary disease (COPD) remains unclear. Methods. Induced sputum was collected from healthy controls and individuals with asthma or COPD. β-defensin-1 protein in sputum supernatant was quantified by ELISA. Biomarker potential was examined using receiver operating characteristic curves. β-defensin-1 release from primary bronchial epithelial cells (pBECs) was investigated in culture with and without cigarette smoke extract (CSE). Results. Airway β-defensin-1 protein was elevated in COPD participants compared to asthma participants and healthy controls. Inflammatory phenotype had no effect on β-defensin-1 levels in asthma or COPD. β-defensin-1 protein was significantly higher in severe asthma compared to controlled and uncontrolled asthma. β-defensin-1 protein could predict the presence of COPD from both healthy controls and asthma patients. Exposure of pBECs to CSE decreased β-defensin-1 production in healthy controls; however in pBECs from COPD participants the level of β-defensin-1 remanied unchanged. Conclusions. Elevated β-defensin-1 protein is a feature of COPD and severe asthma regardless of inflammatory phenotype. β-defensin-1 production is dysregulated in the epithelium of patients with COPD and may be an effective biomarker and potential therapeutic target. |
| format | Article |
| id | doaj-art-c0747b5323564e79b1cec0015f56f190 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c0747b5323564e79b1cec0015f56f1902025-02-03T01:32:05ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/407271407271Airway β-Defensin-1 Protein Is Elevated in COPD and Severe AsthmaKatherine J. Baines0Thomas K. Wright1Jodie L. Simpson2Vanessa M. McDonald3Lisa G. Wood4Kristy S. Parsons5Peter A. Wark6Peter G. Gibson7Priority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaPriority Research Centre for Asthma and Respiratory Diseases, Hunter Medical Research Institute, The University of Newcastle, New Lambton Heights, NSW 2305, AustraliaBackground. Innate immune antimicrobial peptides, including β-defensin-1, promote the chemotaxis and activation of several immune cells. The role of β-defensin-1 in asthma and chronic obstructive pulmonary disease (COPD) remains unclear. Methods. Induced sputum was collected from healthy controls and individuals with asthma or COPD. β-defensin-1 protein in sputum supernatant was quantified by ELISA. Biomarker potential was examined using receiver operating characteristic curves. β-defensin-1 release from primary bronchial epithelial cells (pBECs) was investigated in culture with and without cigarette smoke extract (CSE). Results. Airway β-defensin-1 protein was elevated in COPD participants compared to asthma participants and healthy controls. Inflammatory phenotype had no effect on β-defensin-1 levels in asthma or COPD. β-defensin-1 protein was significantly higher in severe asthma compared to controlled and uncontrolled asthma. β-defensin-1 protein could predict the presence of COPD from both healthy controls and asthma patients. Exposure of pBECs to CSE decreased β-defensin-1 production in healthy controls; however in pBECs from COPD participants the level of β-defensin-1 remanied unchanged. Conclusions. Elevated β-defensin-1 protein is a feature of COPD and severe asthma regardless of inflammatory phenotype. β-defensin-1 production is dysregulated in the epithelium of patients with COPD and may be an effective biomarker and potential therapeutic target.http://dx.doi.org/10.1155/2015/407271 |
| spellingShingle | Katherine J. Baines Thomas K. Wright Jodie L. Simpson Vanessa M. McDonald Lisa G. Wood Kristy S. Parsons Peter A. Wark Peter G. Gibson Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma Mediators of Inflammation |
| title | Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma |
| title_full | Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma |
| title_fullStr | Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma |
| title_full_unstemmed | Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma |
| title_short | Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma |
| title_sort | airway β defensin 1 protein is elevated in copd and severe asthma |
| url | http://dx.doi.org/10.1155/2015/407271 |
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