Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints
Gene (s) for brown fiber trait was transferred into cytoplasmic male-sterile (CMS), maintainer and restorer lines of upland cotton (Gossypium hirsutum L) by hybridization breeding procedure, and three corresponding lines, “Zong A-ZJ12”, “Zong B-ZJ015” and “Zong R-ZJ02” all with brown fiber trait wer...
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Zhejiang University Press
2002-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.01.0001 |
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| author | WANG Xue-de LI Yue-you |
| author_facet | WANG Xue-de LI Yue-you |
| author_sort | WANG Xue-de |
| collection | DOAJ |
| description | Gene (s) for brown fiber trait was transferred into cytoplasmic male-sterile (CMS), maintainer and restorer lines of upland cotton (Gossypium hirsutum L) by hybridization breeding procedure, and three corresponding lines, “Zong A-ZJ12”, “Zong B-ZJ015” and “Zong R-ZJ02” all with brown fiber trait were developed. Sterile line seeds were separately sowed in rows from maintainer or restorer seeds in the ratio 2∶1 (sterile to fertile plant row) in the seed production field in Agricultural Experiment Station of Zhejiang University. Under the condition of natural insect pollination, the seed yield for sterile line seed reproduction was 1948.5 kg/ha and that for hybrid seed production 1 282.5 kg/ha. Lint yield of a hybrid combination “Zongza No. 1” (Zong A-ZJ12 × Zong R-ZJ02) was 1 266.0 kg/ha and showed significant yield heterosis. “Zong A-ZJ12” was a sterile line without pollen, its flower organs, such as anther volume and fresh weight, stigma length, ovule volume and fresh weight, were all noticeably smaller than maintainer and restorer line. DNA fingerprint atlas of three lines and F<sub>1</sub> hybrid for this CMS system was obtained by AFLP technology, in which there are 52 polymorphism fragments using 18 pairs of AFLP primers. This result also showed that polymorphism of DNA fingerprint produced by the AFLP technique was excellent on the fingerprint stability, repetition as well as efficiency. |
| format | Article |
| id | doaj-art-c0693d3eb431406d8d7cd207fa696204 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c0693d3eb431406d8d7cd207fa6962042025-08-20T03:15:38ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552002-01-01281610.3785/1008-9209.2002.01.000110089209Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprintsWANG Xue-deLI Yue-youGene (s) for brown fiber trait was transferred into cytoplasmic male-sterile (CMS), maintainer and restorer lines of upland cotton (Gossypium hirsutum L) by hybridization breeding procedure, and three corresponding lines, “Zong A-ZJ12”, “Zong B-ZJ015” and “Zong R-ZJ02” all with brown fiber trait were developed. Sterile line seeds were separately sowed in rows from maintainer or restorer seeds in the ratio 2∶1 (sterile to fertile plant row) in the seed production field in Agricultural Experiment Station of Zhejiang University. Under the condition of natural insect pollination, the seed yield for sterile line seed reproduction was 1948.5 kg/ha and that for hybrid seed production 1 282.5 kg/ha. Lint yield of a hybrid combination “Zongza No. 1” (Zong A-ZJ12 × Zong R-ZJ02) was 1 266.0 kg/ha and showed significant yield heterosis. “Zong A-ZJ12” was a sterile line without pollen, its flower organs, such as anther volume and fresh weight, stigma length, ovule volume and fresh weight, were all noticeably smaller than maintainer and restorer line. DNA fingerprint atlas of three lines and F<sub>1</sub> hybrid for this CMS system was obtained by AFLP technology, in which there are 52 polymorphism fragments using 18 pairs of AFLP primers. This result also showed that polymorphism of DNA fingerprint produced by the AFLP technique was excellent on the fingerprint stability, repetition as well as efficiency.https://www.academax.com/doi/10.3785/1008-9209.2002.01.0001colored cottonCMSAFLPDNA fingerprint |
| spellingShingle | WANG Xue-de LI Yue-you Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints 浙江大学学报. 农业与生命科学版 colored cotton CMS AFLP DNA fingerprint |
| title | Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints |
| title_full | Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints |
| title_fullStr | Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints |
| title_full_unstemmed | Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints |
| title_short | Development of cytoplasmic male-sterile, maintainer and restorer lines in colored cotton and construction of their DNA fingerprints |
| title_sort | development of cytoplasmic male sterile maintainer and restorer lines in colored cotton and construction of their dna fingerprints |
| topic | colored cotton CMS AFLP DNA fingerprint |
| url | https://www.academax.com/doi/10.3785/1008-9209.2002.01.0001 |
| work_keys_str_mv | AT wangxuede developmentofcytoplasmicmalesterilemaintainerandrestorerlinesincoloredcottonandconstructionoftheirdnafingerprints AT liyueyou developmentofcytoplasmicmalesterilemaintainerandrestorerlinesincoloredcottonandconstructionoftheirdnafingerprints |