3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids
Digital light processing (DLP)-based bioprinting technology holds immense promise for the advancement of hydrogel constructs in biomedical applications. However, creating high-performance hydrogel constructs with this method is still a challenge, as it requires balancing the physicochemical properti...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24004365 |
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| author | Shunyao Zhu Xueyuan Liao Yue Xu Nazi Zhou Yingzi Pan Jinlin Song Taijing Zheng Lin Zhang Liyun Bai Yu Wang Xia Zhou Maling Gou Jie Tao Rui Liu |
| author_facet | Shunyao Zhu Xueyuan Liao Yue Xu Nazi Zhou Yingzi Pan Jinlin Song Taijing Zheng Lin Zhang Liyun Bai Yu Wang Xia Zhou Maling Gou Jie Tao Rui Liu |
| author_sort | Shunyao Zhu |
| collection | DOAJ |
| description | Digital light processing (DLP)-based bioprinting technology holds immense promise for the advancement of hydrogel constructs in biomedical applications. However, creating high-performance hydrogel constructs with this method is still a challenge, as it requires balancing the physicochemical properties of the matrix while also retaining the cellular activity of the encapsulated cells. Herein, we propose a facile and practical strategy for the 3D bioprinting of high-performance hydrogel constructs through the in-situ birth of stem cell spheroids. The strategy is achieved by loading the cell/dextran microdroplets within gelatin methacryloyl (GelMA) emulsion, where dextran functions as a decoy to capture and aggregate the cells for bioprinting while GelMA enables the mechanical support without losing the structural complexity and fidelity. Post-bioprinting, the leaching of dextran results in a smooth curved surface that promotes in-situ birth of spheroids within hydrogel constructs. This process significant enhances differentiation potential of encapsulated stem cells. As a proof-of-concept, we encapsulate dental pulp stem cells (DPSCs) within hydrogel constructs, showcasing their regenerative capabilities in dentin and neovascular-like structures in vivo. The strategy in our study enables high-performance hydrogel tissue construct fabrication with DLP-based bioprinting, which is anticipated to pave a promising way for diverse biomedical applications. |
| format | Article |
| id | doaj-art-c0565066ced449febe573c8a995d1b56 |
| institution | OA Journals |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-c0565066ced449febe573c8a995d1b562025-08-20T02:33:06ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-01-014339240510.1016/j.bioactmat.2024.09.0333D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroidsShunyao Zhu0Xueyuan Liao1Yue Xu2Nazi Zhou3Yingzi Pan4Jinlin Song5Taijing Zheng6Lin Zhang7Liyun Bai8Yu Wang9Xia Zhou10Maling Gou11Jie Tao12Rui Liu13Department of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaCollege of Stomatology, Chongqing Medical University, Chongqing, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, China; State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing, 400042, ChinaState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan, 610065, ChinaDepartment of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, China; Corresponding author.Department of Stomatology, Daping Hospital, Army Medical University (The Third Military Medical University), Chongqing, 400042, China; Corresponding author.Digital light processing (DLP)-based bioprinting technology holds immense promise for the advancement of hydrogel constructs in biomedical applications. However, creating high-performance hydrogel constructs with this method is still a challenge, as it requires balancing the physicochemical properties of the matrix while also retaining the cellular activity of the encapsulated cells. Herein, we propose a facile and practical strategy for the 3D bioprinting of high-performance hydrogel constructs through the in-situ birth of stem cell spheroids. The strategy is achieved by loading the cell/dextran microdroplets within gelatin methacryloyl (GelMA) emulsion, where dextran functions as a decoy to capture and aggregate the cells for bioprinting while GelMA enables the mechanical support without losing the structural complexity and fidelity. Post-bioprinting, the leaching of dextran results in a smooth curved surface that promotes in-situ birth of spheroids within hydrogel constructs. This process significant enhances differentiation potential of encapsulated stem cells. As a proof-of-concept, we encapsulate dental pulp stem cells (DPSCs) within hydrogel constructs, showcasing their regenerative capabilities in dentin and neovascular-like structures in vivo. The strategy in our study enables high-performance hydrogel tissue construct fabrication with DLP-based bioprinting, which is anticipated to pave a promising way for diverse biomedical applications.http://www.sciencedirect.com/science/article/pii/S2452199X24004365Spheroid3D bioprintingCell-concentrated bioinkTissue engineering |
| spellingShingle | Shunyao Zhu Xueyuan Liao Yue Xu Nazi Zhou Yingzi Pan Jinlin Song Taijing Zheng Lin Zhang Liyun Bai Yu Wang Xia Zhou Maling Gou Jie Tao Rui Liu 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids Bioactive Materials Spheroid 3D bioprinting Cell-concentrated bioink Tissue engineering |
| title | 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids |
| title_full | 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids |
| title_fullStr | 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids |
| title_full_unstemmed | 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids |
| title_short | 3D bioprinting of high-performance hydrogel with in-situ birth of stem cell spheroids |
| title_sort | 3d bioprinting of high performance hydrogel with in situ birth of stem cell spheroids |
| topic | Spheroid 3D bioprinting Cell-concentrated bioink Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24004365 |
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