Study on the monoclonal antibody to chlorpyrifos
One hybridoma cell line (1G10) secreting monoclonal antibody (McAb) against chlorpyrifos was produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin (BSA). Isotype and subclass of the monoclonal...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2006-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.06.0591 |
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| author | JIN Ren-yao GUI Wen-jun WU Jian-xiang WANG Chun-mei ZHU Guo-nian |
| author_facet | JIN Ren-yao GUI Wen-jun WU Jian-xiang WANG Chun-mei ZHU Guo-nian |
| author_sort | JIN Ren-yao |
| collection | DOAJ |
| description | One hybridoma cell line (1G10) secreting monoclonal antibody (McAb) against chlorpyrifos was produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin (BSA). Isotype and subclass of the monoclonal cell (1G10) was classified as IgG1. The light chain of the McAb was indentified to be κ. Its titres of ascitic fluids were up to 1 × 10<sup>-6</sup> by indirect ELISA. The inhibition rate of 50% of the McAb with the insecticide chlorpyrifos detected by ELISA was 80.8 μg·L<sup>-1</sup>, the McAb could specifically react with chlorpyrifos but no cross-reactivity to chlorpyrifos analogues. |
| format | Article |
| id | doaj-art-c055fbb76dfb4488a01b24a7b4fc394d |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2006-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c055fbb76dfb4488a01b24a7b4fc394d2025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552006-11-013259159710.3785/1008-9209.2006.06.059110089209Study on the monoclonal antibody to chlorpyrifosJIN Ren-yaoGUI Wen-junWU Jian-xiangWANG Chun-meiZHU Guo-nianOne hybridoma cell line (1G10) secreting monoclonal antibody (McAb) against chlorpyrifos was produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/C which were immunized by the artificial antigen conjugated with bovine serum albumin (BSA). Isotype and subclass of the monoclonal cell (1G10) was classified as IgG1. The light chain of the McAb was indentified to be κ. Its titres of ascitic fluids were up to 1 × 10<sup>-6</sup> by indirect ELISA. The inhibition rate of 50% of the McAb with the insecticide chlorpyrifos detected by ELISA was 80.8 μg·L<sup>-1</sup>, the McAb could specifically react with chlorpyrifos but no cross-reactivity to chlorpyrifos analogues.https://www.academax.com/doi/10.3785/1008-9209.2006.06.0591chlorpyrifosmonoclonal antibodyELISA |
| spellingShingle | JIN Ren-yao GUI Wen-jun WU Jian-xiang WANG Chun-mei ZHU Guo-nian Study on the monoclonal antibody to chlorpyrifos 浙江大学学报. 农业与生命科学版 chlorpyrifos monoclonal antibody ELISA |
| title | Study on the monoclonal antibody to chlorpyrifos |
| title_full | Study on the monoclonal antibody to chlorpyrifos |
| title_fullStr | Study on the monoclonal antibody to chlorpyrifos |
| title_full_unstemmed | Study on the monoclonal antibody to chlorpyrifos |
| title_short | Study on the monoclonal antibody to chlorpyrifos |
| title_sort | study on the monoclonal antibody to chlorpyrifos |
| topic | chlorpyrifos monoclonal antibody ELISA |
| url | https://www.academax.com/doi/10.3785/1008-9209.2006.06.0591 |
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