Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results
There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2011/204723 |
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| author | Zora Haviarová Andrea Janegová Pavel Janega Štefan Durdík Peter Kováč Viera Štvrtinová Peter Mráz |
| author_facet | Zora Haviarová Andrea Janegová Pavel Janega Štefan Durdík Peter Kováč Viera Štvrtinová Peter Mráz |
| author_sort | Zora Haviarová |
| collection | DOAJ |
| description | There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpaired 𝑡-test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works. |
| format | Article |
| id | doaj-art-c044a57b4d0b4e869d3416b25db13a9b |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-c044a57b4d0b4e869d3416b25db13a9b2025-02-03T00:58:59ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322011-01-01201110.1155/2011/204723204723Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary ResultsZora Haviarová0Andrea Janegová1Pavel Janega2Štefan Durdík3Peter Kováč4Viera Štvrtinová5Peter Mráz6Department of Anatomy, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, SlovakiaDepartment of Pathology, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, SlovakiaDepartment of Pathology, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, SlovakiaIst Surgical Clinic, University Hospital and Medical Faculty, Comenius University, Mickiewiczova 13, 81369 Bratislava, SlovakiaDepartment of Forensic Medicine, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, Slovakia2nd Internal Clinic, University Hospital and Faculty of Medicine, Comenius University, Mickiewiczova 13, 81369 Bratislava, SlovakiaDepartment of Anatomy, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, SlovakiaThere are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpaired 𝑡-test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works.http://dx.doi.org/10.1155/2011/204723 |
| spellingShingle | Zora Haviarová Andrea Janegová Pavel Janega Štefan Durdík Peter Kováč Viera Štvrtinová Peter Mráz Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results International Journal of Vascular Medicine |
| title | Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results |
| title_full | Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results |
| title_fullStr | Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results |
| title_full_unstemmed | Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results |
| title_short | Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results |
| title_sort | expression of constitutive nitric oxide synthase isoforms in varicose vein wall preliminary results |
| url | http://dx.doi.org/10.1155/2011/204723 |
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