Establishment of minigenomes for infectious bursal disease virus
Abstract Minigenomes (MGs) have greatly advanced research on the viral life cycle, including viral replication and transcription, virus‒host interactions, and the discovery of antivirals against RNA viruses. However, an MG for infectious bursal disease virus (IBDV) has not been well established. Her...
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BMC
2024-12-01
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| Series: | Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s13567-024-01423-6 |
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| author | Hui Yang Mingrui Zhang Sanying Wang Daxin Peng Luis Martinez-Sobrido Chengjin Ye |
| author_facet | Hui Yang Mingrui Zhang Sanying Wang Daxin Peng Luis Martinez-Sobrido Chengjin Ye |
| author_sort | Hui Yang |
| collection | DOAJ |
| description | Abstract Minigenomes (MGs) have greatly advanced research on the viral life cycle, including viral replication and transcription, virus‒host interactions, and the discovery of antivirals against RNA viruses. However, an MG for infectious bursal disease virus (IBDV) has not been well established. Here, we describe the development of IBDV MG, in which the entire coding sequences of viral genomic segments A and B are replaced with Renilla luciferase (Rluc) or enhanced green fluorescent protein (EGFP) reporter genes. Under the control of the RNA polymerase I promoter, the translation of IBDV MG is controlled by the viral proteins VP1 and VP3. Interestingly, IBDV B MG shows greater activity than does IBDV A MG. Moreover, the sense IBDV B MG was expressed at a higher level than the antisense IBDV B MG. In agreement with our previous findings, the translation of IBDV B MG controlled by VP1 and VP3 is independent of the cellular translation machinery components eukaryotic initiation factor (eIF)4E and eIF4G, but intact VP1 polymerase activity, VP3 dsRNA-binding activity, and the interaction between VP1 and VP3 are indispensable for both sense and antisense IBDV B MG activity. In addition, ribavirin, which inhibits IBDV replication, inhibits IBDV B MG activity in a dose-dependent manner. Collectively, the IBDV MG established in this study provides a powerful tool to investigate IBDV intracellular replication and transcription and virus‒host interactions and facilitates high-throughput screening for the identification of IBDV antivirals. |
| format | Article |
| id | doaj-art-c044a371af134961bae57bce265b0cde |
| institution | OA Journals |
| issn | 1297-9716 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj-art-c044a371af134961bae57bce265b0cde2025-08-20T02:31:50ZengBMCVeterinary Research1297-97162024-12-0155111110.1186/s13567-024-01423-6Establishment of minigenomes for infectious bursal disease virusHui Yang0Mingrui Zhang1Sanying Wang2Daxin Peng3Luis Martinez-Sobrido4Chengjin Ye5Disease Intervention and Prevention Program, Texas Biomedical Research InstituteWenzhou Medical UniversityZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang HospitalCollege of Veterinary Medicine, Yangzhou UniversityDisease Intervention and Prevention Program, Texas Biomedical Research InstituteDisease Intervention and Prevention Program, Texas Biomedical Research InstituteAbstract Minigenomes (MGs) have greatly advanced research on the viral life cycle, including viral replication and transcription, virus‒host interactions, and the discovery of antivirals against RNA viruses. However, an MG for infectious bursal disease virus (IBDV) has not been well established. Here, we describe the development of IBDV MG, in which the entire coding sequences of viral genomic segments A and B are replaced with Renilla luciferase (Rluc) or enhanced green fluorescent protein (EGFP) reporter genes. Under the control of the RNA polymerase I promoter, the translation of IBDV MG is controlled by the viral proteins VP1 and VP3. Interestingly, IBDV B MG shows greater activity than does IBDV A MG. Moreover, the sense IBDV B MG was expressed at a higher level than the antisense IBDV B MG. In agreement with our previous findings, the translation of IBDV B MG controlled by VP1 and VP3 is independent of the cellular translation machinery components eukaryotic initiation factor (eIF)4E and eIF4G, but intact VP1 polymerase activity, VP3 dsRNA-binding activity, and the interaction between VP1 and VP3 are indispensable for both sense and antisense IBDV B MG activity. In addition, ribavirin, which inhibits IBDV replication, inhibits IBDV B MG activity in a dose-dependent manner. Collectively, the IBDV MG established in this study provides a powerful tool to investigate IBDV intracellular replication and transcription and virus‒host interactions and facilitates high-throughput screening for the identification of IBDV antivirals.https://doi.org/10.1186/s13567-024-01423-6IBDVminigenomesVP1VP3reporter geneantivirals |
| spellingShingle | Hui Yang Mingrui Zhang Sanying Wang Daxin Peng Luis Martinez-Sobrido Chengjin Ye Establishment of minigenomes for infectious bursal disease virus Veterinary Research IBDV minigenomes VP1 VP3 reporter gene antivirals |
| title | Establishment of minigenomes for infectious bursal disease virus |
| title_full | Establishment of minigenomes for infectious bursal disease virus |
| title_fullStr | Establishment of minigenomes for infectious bursal disease virus |
| title_full_unstemmed | Establishment of minigenomes for infectious bursal disease virus |
| title_short | Establishment of minigenomes for infectious bursal disease virus |
| title_sort | establishment of minigenomes for infectious bursal disease virus |
| topic | IBDV minigenomes VP1 VP3 reporter gene antivirals |
| url | https://doi.org/10.1186/s13567-024-01423-6 |
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