Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS
Metabolic profiling using nonsputum samples has demonstrated excellent performance in diagnosing infectious diseases. But little is known about the lipid metabolism alternation in children with tuberculosis (TB). Therefore, the study was performed to explore lipid metabolic changes caused by Mycobac...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2023/8111355 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551142095060992 |
|---|---|
| author | Baixu Sun Fang Liu Qingqin Yin Tingting Jiang Min Fang Li Duan Shuting Quan Xue Tian Adong Shen Kaixia Mi Lin Sun |
| author_facet | Baixu Sun Fang Liu Qingqin Yin Tingting Jiang Min Fang Li Duan Shuting Quan Xue Tian Adong Shen Kaixia Mi Lin Sun |
| author_sort | Baixu Sun |
| collection | DOAJ |
| description | Metabolic profiling using nonsputum samples has demonstrated excellent performance in diagnosing infectious diseases. But little is known about the lipid metabolism alternation in children with tuberculosis (TB). Therefore, the study was performed to explore lipid metabolic changes caused by Mycobacterium tuberculosis infection and identify specific lipids as diagnostic biomarkers in children with TB using UHPLC-MS/MS. Plasma samples obtained from 70 active TB children, 21 non-TB infectious disease children, and 21 healthy controls were analyzed by a partial least-squares discriminant analysis model in the training set, and 12 metabolites were identified that can separate children with TB from non-TB controls. In the independent testing cohort with 49 subjects, three of the markers, PC (15:0/17:1), PC (17:1/18:2), and PE (18:1/20:3), presented with high diagnostic values. The areas under the curve of the three metabolites were 0.904, 0.833, and 0.895, respectively. The levels of the altered lipid metabolites were found to be associated with the severity of the TB disease. Taken together, plasma lipid metabolites are potentially useful for diagnosis of active TB in children and would provide insights into the pathogenesis of the disease. |
| format | Article |
| id | doaj-art-c043e9393fd64e70a3ef5899169e8551 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-c043e9393fd64e70a3ef5899169e85512025-02-03T06:04:52ZengWileyJournal of Immunology Research2314-71562023-01-01202310.1155/2023/8111355Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MSBaixu Sun0Fang Liu1Qingqin Yin2Tingting Jiang3Min Fang4Li Duan5Shuting Quan6Xue Tian7Adong Shen8Kaixia Mi9Lin Sun10Beijing Children’s HospitalBeijing Children’s HospitalBeijing Children’s HospitalBaoding Children’s HospitalDepartment of Pediatrics Infectious DiseasesDepartment of Pediatrics Infectious DiseasesBeijing Children’s HospitalBeijing Children’s HospitalBeijing Children’s HospitalCAS Key Laboratory of Pathogenic Microbiology and ImmunologyBeijing Children’s HospitalMetabolic profiling using nonsputum samples has demonstrated excellent performance in diagnosing infectious diseases. But little is known about the lipid metabolism alternation in children with tuberculosis (TB). Therefore, the study was performed to explore lipid metabolic changes caused by Mycobacterium tuberculosis infection and identify specific lipids as diagnostic biomarkers in children with TB using UHPLC-MS/MS. Plasma samples obtained from 70 active TB children, 21 non-TB infectious disease children, and 21 healthy controls were analyzed by a partial least-squares discriminant analysis model in the training set, and 12 metabolites were identified that can separate children with TB from non-TB controls. In the independent testing cohort with 49 subjects, three of the markers, PC (15:0/17:1), PC (17:1/18:2), and PE (18:1/20:3), presented with high diagnostic values. The areas under the curve of the three metabolites were 0.904, 0.833, and 0.895, respectively. The levels of the altered lipid metabolites were found to be associated with the severity of the TB disease. Taken together, plasma lipid metabolites are potentially useful for diagnosis of active TB in children and would provide insights into the pathogenesis of the disease.http://dx.doi.org/10.1155/2023/8111355 |
| spellingShingle | Baixu Sun Fang Liu Qingqin Yin Tingting Jiang Min Fang Li Duan Shuting Quan Xue Tian Adong Shen Kaixia Mi Lin Sun Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS Journal of Immunology Research |
| title | Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS |
| title_full | Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS |
| title_fullStr | Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS |
| title_full_unstemmed | Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS |
| title_short | Exploration of Lipid Metabolism Alterations in Children with Active Tuberculosis Using UHPLC-MS/MS |
| title_sort | exploration of lipid metabolism alterations in children with active tuberculosis using uhplc ms ms |
| url | http://dx.doi.org/10.1155/2023/8111355 |
| work_keys_str_mv | AT baixusun explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT fangliu explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT qingqinyin explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT tingtingjiang explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT minfang explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT liduan explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT shutingquan explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT xuetian explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT adongshen explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT kaixiami explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms AT linsun explorationoflipidmetabolismalterationsinchildrenwithactivetuberculosisusinguhplcmsms |