Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center
Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 inte...
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The Journal of Infection in Developing Countries
2021-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12996 |
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| author | Iliana Alejandra Cortés-Ortíz Julio Cesar Juárez-Gómez Concepción Cu-Quijano Rocio Flores-Paz Emilio Mariano Durán-Manuel Clemente Cruz-Cruz Victor Hugo Gutiérrez-Muñoz Oscar Sosa-Hernández Noé Escobar-Escamilla Juan Carlos Bravata-Alcántara Laura Delgado-Balbuena Gabriela Ibáñez-Cervantes Juan Manuel Bello-López |
| author_facet | Iliana Alejandra Cortés-Ortíz Julio Cesar Juárez-Gómez Concepción Cu-Quijano Rocio Flores-Paz Emilio Mariano Durán-Manuel Clemente Cruz-Cruz Victor Hugo Gutiérrez-Muñoz Oscar Sosa-Hernández Noé Escobar-Escamilla Juan Carlos Bravata-Alcántara Laura Delgado-Balbuena Gabriela Ibáñez-Cervantes Juan Manuel Bello-López |
| author_sort | Iliana Alejandra Cortés-Ortíz |
| collection | DOAJ |
| description |
Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak.
Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed.
Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”.
Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel.
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| format | Article |
| id | doaj-art-c0341c70b01f4bc9877355c10eed23d8 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-c0341c70b01f4bc9877355c10eed23d82025-08-20T03:52:41ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-05-01150510.3855/jidc.12996Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention centerIliana Alejandra Cortés-Ortíz0Julio Cesar Juárez-Gómez1Concepción Cu-Quijano2Rocio Flores-Paz3Emilio Mariano Durán-Manuel4Clemente Cruz-Cruz5Victor Hugo Gutiérrez-Muñoz6Oscar Sosa-Hernández7Noé Escobar-Escamilla8Juan Carlos Bravata-Alcántara9Laura Delgado-Balbuena10Gabriela Ibáñez-Cervantes11Juan Manuel Bello-López12Laboratory of Bacteriology, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoLaboratory of Bacteriology, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoLaboratory of Bacteriology, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoLaboratory of Bacteriology, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoResearch Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoResearch Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoHospital Epidemiological Surveillance Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoHospital Epidemiological Surveillance Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoInstituto de Diagnóstico y Referencia Epidemiológicos "Dr. Manuel Martínez Báez", Francisco de P. Miranda, Mexico City, MexicoMolecular Diagnostic Laboratory, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoResearch Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoResearch Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, MexicoResearch Unit, Hospital Juárez de México, Instituto Politécnico Nacional, Mexico City, Mexico Introduction: Infections acquired in hospitals are the cause of high morbidity and mortality and with the emergence of resistant bacteria, the problem is greater. The aim of this work was to determine the genetic characteristics and timeline of Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron involved in an intrahospital outbreak. Methodology: Investigation was made from the first detection of K. pneumoniae blaNDM-1, strain “466”, and the last clone “423”. 16S rRNA gene analysis showed that 466 strain and clones were related to K. pneumoniae. Extended-spectrum β-lactamases (ESBL) was detected according to the Clinical and Laboratory Standards Institute (CLSI) and real time-PCR. Typing of K. pneumoniae blaNDM-1 strains was carried by ERIC-PCR and sequencing the variable region of the integrons were performed. Results: A cluster of six resistant isolates of K. pneumoniae blaNDM-1 was detected in intensive care unit (ICU), internal medicine (IM) and orthopedics (OT). Timeline revealed that the first bacterial identification was in ICU and the last clone in OT service. The array genetic of variable region was “IntI/aadA5-drfA17/qacEΔ1-Sul1”. Conclusions: The evidences highlight the importance of the epidemiological surveillance of Extended-spectrum β-lactamases (ESBL) strains, as well as the need for molecular epidemiological studies to identify the routes of transmission and the contamination sources within health personnel. https://jidc.org/index.php/journal/article/view/12996Hospital outbreakKlebsiella pneumoniaeblaNDM-1class 1 integron |
| spellingShingle | Iliana Alejandra Cortés-Ortíz Julio Cesar Juárez-Gómez Concepción Cu-Quijano Rocio Flores-Paz Emilio Mariano Durán-Manuel Clemente Cruz-Cruz Victor Hugo Gutiérrez-Muñoz Oscar Sosa-Hernández Noé Escobar-Escamilla Juan Carlos Bravata-Alcántara Laura Delgado-Balbuena Gabriela Ibáñez-Cervantes Juan Manuel Bello-López Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center Journal of Infection in Developing Countries Hospital outbreak Klebsiella pneumoniae blaNDM-1 class 1 integron |
| title | Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center |
| title_full | Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center |
| title_fullStr | Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center |
| title_full_unstemmed | Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center |
| title_short | Klebsiella pneumoniae blaNDM-1 carrying a class 1 integron causing a hospital outbreak in a Mexican attention center |
| title_sort | klebsiella pneumoniae blandm 1 carrying a class 1 integron causing a hospital outbreak in a mexican attention center |
| topic | Hospital outbreak Klebsiella pneumoniae blaNDM-1 class 1 integron |
| url | https://jidc.org/index.php/journal/article/view/12996 |
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