First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt

Abstract Objective This study aimed to assess the prevalence of Cryptosporidium genotypes in the canal water bodies of Minya Al-Qamh District in Sharqia Governorate, Northern Egypt. Rural populations in Egypt lack access to clean water. They obtain their water supplies from different drains and cana...

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Main Authors: Marwa Omar, Samia E. Etewa, Tahani I. Farag, Samar Abd EL-Nabi, Heba Abdelal
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Research Notes
Subjects:
Online Access:https://doi.org/10.1186/s13104-025-07360-7
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author Marwa Omar
Samia E. Etewa
Tahani I. Farag
Samar Abd EL-Nabi
Heba Abdelal
author_facet Marwa Omar
Samia E. Etewa
Tahani I. Farag
Samar Abd EL-Nabi
Heba Abdelal
author_sort Marwa Omar
collection DOAJ
description Abstract Objective This study aimed to assess the prevalence of Cryptosporidium genotypes in the canal water bodies of Minya Al-Qamh District in Sharqia Governorate, Northern Egypt. Rural populations in Egypt lack access to clean water. They obtain their water supplies from different drains and canals, which are frequently exposed to contamination by human activities and untreated agricultural waste, introducing Cryptosporidium infection to unprotected waterways. A total of (72) canal water samples served for the molecular detection of Cryptosporidium species by PCR amplification and sequencing. Results Only one sample 1.4% (1/72) belonging to the village of Al-Aziziyyah was PCR-positive for Cryptosporidium contamination. Based on the (COWP) gene sequencing, this species was identified as Cryptosporidium parvum (C. parvum). The current work’s findings marked the first report on the molecular characterization of Cryptosporidium species in the canal water of Sharqia Province. Our results suggested that the source of C. parvum contamination could be of human and/or animal origin. Therefore, further studies are warranted to track the source of human infection and mitigate contamination for improved water quality.
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institution Kabale University
issn 1756-0500
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publishDate 2025-07-01
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spelling doaj-art-c033f1c8215046fdbf66673f1fb03d722025-08-20T03:42:19ZengBMCBMC Research Notes1756-05002025-07-011811710.1186/s13104-025-07360-7First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern EgyptMarwa Omar0Samia E. Etewa1Tahani I. Farag2Samar Abd EL-Nabi3Heba Abdelal4Department of Medical Parasitology, Faculty of Medicine, Zagazig UniversityDepartment of Medical Parasitology, Faculty of Medicine, Zagazig UniversityDepartment of Medical Parasitology, Faculty of Medicine, Zagazig UniversityDepartment of Medical Parasitology, Faculty of Medicine, Zagazig UniversityMaison des Sciences Humaines, LIS: Cross-National Data CenterAbstract Objective This study aimed to assess the prevalence of Cryptosporidium genotypes in the canal water bodies of Minya Al-Qamh District in Sharqia Governorate, Northern Egypt. Rural populations in Egypt lack access to clean water. They obtain their water supplies from different drains and canals, which are frequently exposed to contamination by human activities and untreated agricultural waste, introducing Cryptosporidium infection to unprotected waterways. A total of (72) canal water samples served for the molecular detection of Cryptosporidium species by PCR amplification and sequencing. Results Only one sample 1.4% (1/72) belonging to the village of Al-Aziziyyah was PCR-positive for Cryptosporidium contamination. Based on the (COWP) gene sequencing, this species was identified as Cryptosporidium parvum (C. parvum). The current work’s findings marked the first report on the molecular characterization of Cryptosporidium species in the canal water of Sharqia Province. Our results suggested that the source of C. parvum contamination could be of human and/or animal origin. Therefore, further studies are warranted to track the source of human infection and mitigate contamination for improved water quality.https://doi.org/10.1186/s13104-025-07360-7CryptosporidiumEgyptGenotypingMinya Al-QamhContaminationCanal water
spellingShingle Marwa Omar
Samia E. Etewa
Tahani I. Farag
Samar Abd EL-Nabi
Heba Abdelal
First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
BMC Research Notes
Cryptosporidium
Egypt
Genotyping
Minya Al-Qamh
Contamination
Canal water
title First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
title_full First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
title_fullStr First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
title_full_unstemmed First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
title_short First molecular identification of Cryptosporidium species isolated from canal water bodies in Minya Al-Qamh district, Northern Egypt
title_sort first molecular identification of cryptosporidium species isolated from canal water bodies in minya al qamh district northern egypt
topic Cryptosporidium
Egypt
Genotyping
Minya Al-Qamh
Contamination
Canal water
url https://doi.org/10.1186/s13104-025-07360-7
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