Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1
Rui Li,1,* Zhibin Huang,2,* Mengmeng Chen3 1Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 2Medical Room, Nantong College of Science and Technology, Nantong, People’s Republic of China; 3Department of Stomatology, The Fourth...
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Dove Medical Press
2025-02-01
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| description | Rui Li,1,* Zhibin Huang,2,* Mengmeng Chen3 1Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 2Medical Room, Nantong College of Science and Technology, Nantong, People’s Republic of China; 3Department of Stomatology, The Fourth Hospital of Harbin, Harbin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mengmeng Chen, Department of Stomatology, The Fourth Hospital of Harbin, No. 119, Jingyu Street, Daowai District, Harbin City, Heilongjiang Province, 150026, People’s Republic of China, Tel +86 0451-88029850, Email chenmengm74@163.comBackground: Several long non-coding RNAs (lncRNAs) are dysregulated in chronic periodontitis (CP).Purpose: The study aimed to elucidate the molecular mechanisms and clinical significance of lncRNA EPB41L4A antisense RNA 1 (EPB41L4A-AS1) in CP.Patients and Methods: This study enrolled 101 patients with CP and 90 subjects with healthy periodontal tissues. Patients with CP were categorized according to severity. The expression of EPB41L4A-AS1 and osteogenic markers in the lipopolysaccharide (LPS)-induced human periodontal ligament cells (hPDLCs) was assessed using real-time quantitative reverse transcription PCR (RT-qPCR). The diagnostic significance of EPB41L4A-AS1 was evaluated using receiver operating characteristic (ROC) analysis. The levels of inflammatory factors were measured using an enzyme-linked immunosorbent assay. Cell proliferation and apoptosis were analyzed using cell counting kit − 8 and flow cytometry, respectively. The interaction between EPB41L4A-AS1 and microRNAs was verified using dual luciferase reporter assays, RNA immunoprecipitation, and RNA pull-down assays.Results: EPB41L4A-AS1 was downregulated in the gingival sulcus fluid of patients with CP and LPS-induced hPDLCs. Additionally, EPB41L4A-AS1 could distinguish patients with CP from control subjects with sensitivity (88.12%) and specificity (81.11%). The expression of EPB41L4A-AS1 was downregulated in patients with severe CP. EPB41L4A-AS1 downregulation was directly correlated with severe clinical indicators and inversely correlated with inflammatory indicators. The overexpression of EPB41L4A-AS1 promoted the proliferation and osteogenic differentiation of hPDLCs and mitigated LPS-induced inflammation. Mechanistically, EPB41L4A-AS1 directly targets and downregulates miR-214-3p expression, resulting in the upregulation of Yes1-associated transcriptional regulator (YAP1) levels. The overexpression of miR-214-3p partially suppressed the effects of EPB41L4A-AS1 on LPS-induced hPDLC injury and osteogenic differentiation.Conclusion: The overexpression of EPB41L4A-AS1 suppressed LPS-induced hPDLC injury and enhanced osteogenic differentiation through the miR-214-3p/YAP1 axis. Thus, EPB41L4A-AS1 is a novel diagnostic marker and a therapeutic target for CP.Keywords: EPB41L4A-AS1, miR-214-3p, osteogenic differentiation, chronic periodontitis |
| format | Article |
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| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-c015604b30c64cf286ebb6ac0b6a97fb2025-08-20T02:14:31ZengDove Medical PressJournal of Inflammation Research1178-70312025-02-01Volume 1824832497100356Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1Li RHuang ZChen MRui Li,1,* Zhibin Huang,2,* Mengmeng Chen3 1Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of China; 2Medical Room, Nantong College of Science and Technology, Nantong, People’s Republic of China; 3Department of Stomatology, The Fourth Hospital of Harbin, Harbin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Mengmeng Chen, Department of Stomatology, The Fourth Hospital of Harbin, No. 119, Jingyu Street, Daowai District, Harbin City, Heilongjiang Province, 150026, People’s Republic of China, Tel +86 0451-88029850, Email chenmengm74@163.comBackground: Several long non-coding RNAs (lncRNAs) are dysregulated in chronic periodontitis (CP).Purpose: The study aimed to elucidate the molecular mechanisms and clinical significance of lncRNA EPB41L4A antisense RNA 1 (EPB41L4A-AS1) in CP.Patients and Methods: This study enrolled 101 patients with CP and 90 subjects with healthy periodontal tissues. Patients with CP were categorized according to severity. The expression of EPB41L4A-AS1 and osteogenic markers in the lipopolysaccharide (LPS)-induced human periodontal ligament cells (hPDLCs) was assessed using real-time quantitative reverse transcription PCR (RT-qPCR). The diagnostic significance of EPB41L4A-AS1 was evaluated using receiver operating characteristic (ROC) analysis. The levels of inflammatory factors were measured using an enzyme-linked immunosorbent assay. Cell proliferation and apoptosis were analyzed using cell counting kit − 8 and flow cytometry, respectively. The interaction between EPB41L4A-AS1 and microRNAs was verified using dual luciferase reporter assays, RNA immunoprecipitation, and RNA pull-down assays.Results: EPB41L4A-AS1 was downregulated in the gingival sulcus fluid of patients with CP and LPS-induced hPDLCs. Additionally, EPB41L4A-AS1 could distinguish patients with CP from control subjects with sensitivity (88.12%) and specificity (81.11%). The expression of EPB41L4A-AS1 was downregulated in patients with severe CP. EPB41L4A-AS1 downregulation was directly correlated with severe clinical indicators and inversely correlated with inflammatory indicators. The overexpression of EPB41L4A-AS1 promoted the proliferation and osteogenic differentiation of hPDLCs and mitigated LPS-induced inflammation. Mechanistically, EPB41L4A-AS1 directly targets and downregulates miR-214-3p expression, resulting in the upregulation of Yes1-associated transcriptional regulator (YAP1) levels. The overexpression of miR-214-3p partially suppressed the effects of EPB41L4A-AS1 on LPS-induced hPDLC injury and osteogenic differentiation.Conclusion: The overexpression of EPB41L4A-AS1 suppressed LPS-induced hPDLC injury and enhanced osteogenic differentiation through the miR-214-3p/YAP1 axis. Thus, EPB41L4A-AS1 is a novel diagnostic marker and a therapeutic target for CP.Keywords: EPB41L4A-AS1, miR-214-3p, osteogenic differentiation, chronic periodontitishttps://www.dovepress.com/long-non-coding-rna-epb41l4a-as1-serves-as-a-diagnostic-marker-for-chr-peer-reviewed-fulltext-article-JIRepb41l4a-as1mir-214-3posteogenic differentiationchronic periodontitis |
| spellingShingle | Li R Huang Z Chen M Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 Journal of Inflammation Research epb41l4a-as1 mir-214-3p osteogenic differentiation chronic periodontitis |
| title | Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 |
| title_full | Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 |
| title_fullStr | Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 |
| title_full_unstemmed | Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 |
| title_short | Long Non-Coding RNA EPB41L4A-AS1 Serves as a Diagnostic Marker for Chronic Periodontitis and Regulates Periodontal Ligament Injury and Osteogenic Differentiation by Targeting miR-214-3p/YAP1 |
| title_sort | long non coding rna epb41l4a as1 serves as a diagnostic marker for chronic periodontitis and regulates periodontal ligament injury and osteogenic differentiation by targeting mir 214 3p yap1 |
| topic | epb41l4a-as1 mir-214-3p osteogenic differentiation chronic periodontitis |
| url | https://www.dovepress.com/long-non-coding-rna-epb41l4a-as1-serves-as-a-diagnostic-marker-for-chr-peer-reviewed-fulltext-article-JIR |
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