Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing

Eighty percent of asthma attacks in children are accompanied by an upper respiratory tract viral infection. Adenovirus is one of the major viral causes of childhood bronchiolitis. As the polymerase chain reaction (PCR) is the most sensitive technique for documenting viral respiratory infectio...

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Main Authors: Cansin Saçkesen, Ahmet Pinar, Bülent E Sekerel, Yakut Akyön, Yildiz Saraçlar
Format: Article
Language:English
Published: Hacettepe University Institute of Child Health 2005-07-01
Series:The Turkish Journal of Pediatrics
Online Access:https://turkjpediatr.org/article/view/2719
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author Cansin Saçkesen
Ahmet Pinar
Bülent E Sekerel
Yakut Akyön
Yildiz Saraçlar
author_facet Cansin Saçkesen
Ahmet Pinar
Bülent E Sekerel
Yakut Akyön
Yildiz Saraçlar
author_sort Cansin Saçkesen
collection DOAJ
description Eighty percent of asthma attacks in children are accompanied by an upper respiratory tract viral infection. Adenovirus is one of the major viral causes of childhood bronchiolitis. As the polymerase chain reaction (PCR) is the most sensitive technique for documenting viral respiratory infections, the PCR method was performed on the throat swab samples of asthmatic children with and without wheezing to investigate the presence of the adenovirus genome in the upper respiratory tract. The frequencies of adenovirus in asymptomatic and symptomatic asthmatic patients, healthy controls and wheezy children were as follows: 33.3%, 71.4%, 37% and 62.96%, respectively. The adenovirus was detected in a significantly higher percentage in the upper airways of patients with asthma exacerbation and in children with wheezing than in patients without asthma exacerbation and in the healthy controls (p < 0.05). The frequency of adenovirus was not different between asthmatic patients receiving or not receiving inhaled corticosteroid. Adenovirus has the potential to precipitate asthma exacerbations in asthmatic patients; its frequency was not affected by the treatment of inhaled corticosteroid.
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issn 0041-4301
2791-6421
language English
publishDate 2005-07-01
publisher Hacettepe University Institute of Child Health
record_format Article
series The Turkish Journal of Pediatrics
spelling doaj-art-c0128e6a644f4c88a192be6e5bf47f2a2025-08-20T02:01:50ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212005-07-01473Use of polymerase chain reaction for detection of adenovirus in children with or without wheezingCansin Saçkesen0Ahmet PinarBülent E SekerelYakut AkyönYildiz SaraçlarDepartment of Pediatrics, Hacettepe University Faculty of Medicine, Ankara, Turkey. Eighty percent of asthma attacks in children are accompanied by an upper respiratory tract viral infection. Adenovirus is one of the major viral causes of childhood bronchiolitis. As the polymerase chain reaction (PCR) is the most sensitive technique for documenting viral respiratory infections, the PCR method was performed on the throat swab samples of asthmatic children with and without wheezing to investigate the presence of the adenovirus genome in the upper respiratory tract. The frequencies of adenovirus in asymptomatic and symptomatic asthmatic patients, healthy controls and wheezy children were as follows: 33.3%, 71.4%, 37% and 62.96%, respectively. The adenovirus was detected in a significantly higher percentage in the upper airways of patients with asthma exacerbation and in children with wheezing than in patients without asthma exacerbation and in the healthy controls (p < 0.05). The frequency of adenovirus was not different between asthmatic patients receiving or not receiving inhaled corticosteroid. Adenovirus has the potential to precipitate asthma exacerbations in asthmatic patients; its frequency was not affected by the treatment of inhaled corticosteroid. https://turkjpediatr.org/article/view/2719
spellingShingle Cansin Saçkesen
Ahmet Pinar
Bülent E Sekerel
Yakut Akyön
Yildiz Saraçlar
Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
The Turkish Journal of Pediatrics
title Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
title_full Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
title_fullStr Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
title_full_unstemmed Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
title_short Use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
title_sort use of polymerase chain reaction for detection of adenovirus in children with or without wheezing
url https://turkjpediatr.org/article/view/2719
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AT bulentesekerel useofpolymerasechainreactionfordetectionofadenovirusinchildrenwithorwithoutwheezing
AT yakutakyon useofpolymerasechainreactionfordetectionofadenovirusinchildrenwithorwithoutwheezing
AT yildizsaraclar useofpolymerasechainreactionfordetectionofadenovirusinchildrenwithorwithoutwheezing