Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea.
Clostridium difficile infections are an emerging health problem in the modern hospital environment. Severe alterations of the gut microbiome with loss of resistance to colonization against C. difficile are thought to be the major trigger, but there is no clear concept of how C. difficile infection e...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089417&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850189912400199680 |
|---|---|
| author | Henrik Knecht Sven C Neulinger Femke Anouska Heinsen Carolin Knecht Anke Schilhabel Ruth A Schmitz Alexandra Zimmermann Vitor Martins dos Santos Manuel Ferrer Philip C Rosenstiel Stefan Schreiber Anette K Friedrichs Stephan J Ott |
| author_facet | Henrik Knecht Sven C Neulinger Femke Anouska Heinsen Carolin Knecht Anke Schilhabel Ruth A Schmitz Alexandra Zimmermann Vitor Martins dos Santos Manuel Ferrer Philip C Rosenstiel Stefan Schreiber Anette K Friedrichs Stephan J Ott |
| author_sort | Henrik Knecht |
| collection | DOAJ |
| description | Clostridium difficile infections are an emerging health problem in the modern hospital environment. Severe alterations of the gut microbiome with loss of resistance to colonization against C. difficile are thought to be the major trigger, but there is no clear concept of how C. difficile infection evolves and which microbiological factors are involved. We sequenced 16S rRNA amplicons generated from DNA and RNA/cDNA of fecal samples from three groups of individuals by FLX technology: (i) healthy controls (no antibiotic therapy); (ii) individuals receiving antibiotic therapy (Ampicillin/Sulbactam, cephalosporins, and fluoroquinolones with subsequent development of C. difficile infection or (iii) individuals receiving antibiotic therapy without C. difficile infection. We compared the effects of the three different antibiotic classes on the intestinal microbiome and the effects of alterations of the gut microbiome on C. difficile infection at the DNA (total microbiota) and rRNA (potentially active) levels. A comparison of antibiotic classes showed significant differences at DNA level, but not at RNA level. Among individuals that developed or did not develop a C. difficile infection under antibiotics we found no significant differences. We identified single species that were up- or down regulated in individuals receiving antibiotics who developed the infection compared to non-infected individuals. We found no significant differences in the global composition of the transcriptionally active gut microbiome associated with C. difficile infections. We suggest that up- and down regulation of specific bacterial species may be involved in colonization resistance against C. difficile providing a potential therapeutic approach through specific manipulation of the intestinal microbiome. |
| format | Article |
| id | doaj-art-c010fa2eacee4e30a735966fc5c9e554 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c010fa2eacee4e30a735966fc5c9e5542025-08-20T02:15:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8941710.1371/journal.pone.0089417Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea.Henrik KnechtSven C NeulingerFemke Anouska HeinsenCarolin KnechtAnke SchilhabelRuth A SchmitzAlexandra ZimmermannVitor Martins dos SantosManuel FerrerPhilip C RosenstielStefan SchreiberAnette K FriedrichsStephan J OttClostridium difficile infections are an emerging health problem in the modern hospital environment. Severe alterations of the gut microbiome with loss of resistance to colonization against C. difficile are thought to be the major trigger, but there is no clear concept of how C. difficile infection evolves and which microbiological factors are involved. We sequenced 16S rRNA amplicons generated from DNA and RNA/cDNA of fecal samples from three groups of individuals by FLX technology: (i) healthy controls (no antibiotic therapy); (ii) individuals receiving antibiotic therapy (Ampicillin/Sulbactam, cephalosporins, and fluoroquinolones with subsequent development of C. difficile infection or (iii) individuals receiving antibiotic therapy without C. difficile infection. We compared the effects of the three different antibiotic classes on the intestinal microbiome and the effects of alterations of the gut microbiome on C. difficile infection at the DNA (total microbiota) and rRNA (potentially active) levels. A comparison of antibiotic classes showed significant differences at DNA level, but not at RNA level. Among individuals that developed or did not develop a C. difficile infection under antibiotics we found no significant differences. We identified single species that were up- or down regulated in individuals receiving antibiotics who developed the infection compared to non-infected individuals. We found no significant differences in the global composition of the transcriptionally active gut microbiome associated with C. difficile infections. We suggest that up- and down regulation of specific bacterial species may be involved in colonization resistance against C. difficile providing a potential therapeutic approach through specific manipulation of the intestinal microbiome.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089417&type=printable |
| spellingShingle | Henrik Knecht Sven C Neulinger Femke Anouska Heinsen Carolin Knecht Anke Schilhabel Ruth A Schmitz Alexandra Zimmermann Vitor Martins dos Santos Manuel Ferrer Philip C Rosenstiel Stefan Schreiber Anette K Friedrichs Stephan J Ott Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. PLoS ONE |
| title | Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. |
| title_full | Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. |
| title_fullStr | Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. |
| title_full_unstemmed | Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. |
| title_short | Effects of β-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile associated diarrhea. |
| title_sort | effects of β lactam antibiotics and fluoroquinolones on human gut microbiota in relation to clostridium difficile associated diarrhea |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089417&type=printable |
| work_keys_str_mv | AT henrikknecht effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT svencneulinger effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT femkeanouskaheinsen effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT carolinknecht effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT ankeschilhabel effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT ruthaschmitz effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT alexandrazimmermann effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT vitormartinsdossantos effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT manuelferrer effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT philipcrosenstiel effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT stefanschreiber effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT anettekfriedrichs effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea AT stephanjott effectsofblactamantibioticsandfluoroquinolonesonhumangutmicrobiotainrelationtoclostridiumdifficileassociateddiarrhea |