Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro
Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase anaphylactic reaction, and haematopoiesis. Lipopolysaccharide (6–24 μg/ml) significantly induced IL-6 release from murine spleen cells. In cultured rabbit synovial cells interleukin-1 (IL-1, 1–10 U/ml)...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000226 |
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| author | Dian-Wen Ju Qin-Yue Zheng Xue-Tao Cao Qing-Hua Zheng Xiao-Jun Guan Hong-Bin Wang |
| author_facet | Dian-Wen Ju Qin-Yue Zheng Xue-Tao Cao Qing-Hua Zheng Xiao-Jun Guan Hong-Bin Wang |
| author_sort | Dian-Wen Ju |
| collection | DOAJ |
| description | Interleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase anaphylactic reaction, and haematopoiesis. Lipopolysaccharide (6–24 μg/ml) significantly induced IL-6 release from murine spleen cells. In cultured rabbit synovial cells interleukin-1 (IL-1, 1–10 U/ml) induced IL-6 production in a concentration-dependent manner. Triazolodiazepine (Tri) is a hetrazepine platelet-activating factor antagonist. In this study we found that Tri (0.1–10 μmol/l) exerted strong inhibitory effects on LPS stimulated IL-6 production in murine spleen cells. Kinetic studies showed that the inhibition of IL-6 release was time-independent. In rabbit synovial cells Tri also reduced IL-6 release induced by IL-1 and tumour necrosis factor. Inhibition of cytokine production by Tri may partially explain its wide and strong anti-inflammatory effects. |
| format | Article |
| id | doaj-art-c00647765bb54866bbead20b4c9a3e94 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c00647765bb54866bbead20b4c9a3e942025-08-20T02:19:26ZengWileyMediators of Inflammation0962-93511466-18611995-01-014213013210.1155/S0962935195000226Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitroDian-Wen Ju0Qin-Yue Zheng1Xue-Tao Cao2Qing-Hua Zheng3Xiao-Jun Guan4Hong-Bin Wang5Department of Immunology, School of Basic Medicine, Second Military Medical University, Shanghai 200433, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, ChinaDepartment of Immunology, School of Basic Medicine, Second Military Medical University, Shanghai 200433, ChinaDepartment of Immunology, School of Basic Medicine, Second Military Medical University, Shanghai 200433, ChinaDepartment of Immunology, School of Basic Medicine, Second Military Medical University, Shanghai 200433, ChinaDepartment of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, ChinaInterleukin-6 (IL-6) is a multifunctional cytokine that regulates the immune response, acute phase anaphylactic reaction, and haematopoiesis. Lipopolysaccharide (6–24 μg/ml) significantly induced IL-6 release from murine spleen cells. In cultured rabbit synovial cells interleukin-1 (IL-1, 1–10 U/ml) induced IL-6 production in a concentration-dependent manner. Triazolodiazepine (Tri) is a hetrazepine platelet-activating factor antagonist. In this study we found that Tri (0.1–10 μmol/l) exerted strong inhibitory effects on LPS stimulated IL-6 production in murine spleen cells. Kinetic studies showed that the inhibition of IL-6 release was time-independent. In rabbit synovial cells Tri also reduced IL-6 release induced by IL-1 and tumour necrosis factor. Inhibition of cytokine production by Tri may partially explain its wide and strong anti-inflammatory effects.http://dx.doi.org/10.1155/S0962935195000226 |
| spellingShingle | Dian-Wen Ju Qin-Yue Zheng Xue-Tao Cao Qing-Hua Zheng Xiao-Jun Guan Hong-Bin Wang Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro Mediators of Inflammation |
| title | Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro |
| title_full | Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro |
| title_fullStr | Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro |
| title_full_unstemmed | Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro |
| title_short | Effects of triazolodiazepine on the production of interleukin-6 from murine spleen cells and rabbit synovial cells in vitro |
| title_sort | effects of triazolodiazepine on the production of interleukin 6 from murine spleen cells and rabbit synovial cells in vitro |
| url | http://dx.doi.org/10.1155/S0962935195000226 |
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