Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress
Summary: Cadmium (Cd), a highly toxic non-essential heavy metal, disrupts cellular processes even at low concentrations. The current studies of Cd stress rely on whole-plant models with prolonged treatments, obscuring early response signals. Tobacco, a typical model plant, lacks protoplast-level inv...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225015986 |
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| author | Chun Wang Huina Zhou Niu Zhai Qiansi Chen Zefeng Li Chen Wang Qingxia Zheng Ang Li Qi Shen Weimin Shi Pingping Liu |
| author_facet | Chun Wang Huina Zhou Niu Zhai Qiansi Chen Zefeng Li Chen Wang Qingxia Zheng Ang Li Qi Shen Weimin Shi Pingping Liu |
| author_sort | Chun Wang |
| collection | DOAJ |
| description | Summary: Cadmium (Cd), a highly toxic non-essential heavy metal, disrupts cellular processes even at low concentrations. The current studies of Cd stress rely on whole-plant models with prolonged treatments, obscuring early response signals. Tobacco, a typical model plant, lacks protoplast-level investigations under Cd stress. Here, we integrated SWATH-based LC-MS proteomics and GC-MS metabolomics to resolve early Cd-responsive mechanisms in tobacco protoplasts, uniting bioinformatics analyses (WGCNA, k-means, PPI networks). 3262 proteins and 131 primary metabolites were identified. The hub proteins (ROS scavengers, protein synthesis) and differentially expressed metabolites, transient monosaccharide depletion (15 min) followed by recovery, and sustained organic acid accumulation-were characterized. The results indicate rapid energy and some metabolites were supplied via glycolysis and the TCA cycle to alleviate oxidative damage and enhance stress protein synthesis. This study results deciphers tobacco’s multi-omics defense strategy against Cd stress, filling knowledge gaps in early stress signaling. In the future, we anticipate that our study will contribute to advancing protoplast omics. |
| format | Article |
| id | doaj-art-c004c9d6a2644d8b9bbfc4846527f3cc |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-c004c9d6a2644d8b9bbfc4846527f3cc2025-08-24T05:14:04ZengElsevieriScience2589-00422025-09-0128911333710.1016/j.isci.2025.113337Proteomics and metabolomics analyses of tobacco protoplasts under Cd stressChun Wang0Huina Zhou1Niu Zhai2Qiansi Chen3Zefeng Li4Chen Wang5Qingxia Zheng6Ang Li7Qi Shen8Weimin Shi9Pingping Liu10College of Chemistry, Zhengzhou University, Zhengzhou 450000, China; Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, ChinaCollege of Chemistry, Zhengzhou University, Zhengzhou 450000, ChinaCollege of Chemistry, Zhengzhou University, Zhengzhou 450000, ChinaCollege of Chemistry, Zhengzhou University, Zhengzhou 450000, China; Corresponding authorZhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450000, China; Corresponding authorSummary: Cadmium (Cd), a highly toxic non-essential heavy metal, disrupts cellular processes even at low concentrations. The current studies of Cd stress rely on whole-plant models with prolonged treatments, obscuring early response signals. Tobacco, a typical model plant, lacks protoplast-level investigations under Cd stress. Here, we integrated SWATH-based LC-MS proteomics and GC-MS metabolomics to resolve early Cd-responsive mechanisms in tobacco protoplasts, uniting bioinformatics analyses (WGCNA, k-means, PPI networks). 3262 proteins and 131 primary metabolites were identified. The hub proteins (ROS scavengers, protein synthesis) and differentially expressed metabolites, transient monosaccharide depletion (15 min) followed by recovery, and sustained organic acid accumulation-were characterized. The results indicate rapid energy and some metabolites were supplied via glycolysis and the TCA cycle to alleviate oxidative damage and enhance stress protein synthesis. This study results deciphers tobacco’s multi-omics defense strategy against Cd stress, filling knowledge gaps in early stress signaling. In the future, we anticipate that our study will contribute to advancing protoplast omics.http://www.sciencedirect.com/science/article/pii/S2589004225015986Natural sciencesPlant biochemistryPlant bioinformaticsPlant BiologyPlant physiologyPlant systematics |
| spellingShingle | Chun Wang Huina Zhou Niu Zhai Qiansi Chen Zefeng Li Chen Wang Qingxia Zheng Ang Li Qi Shen Weimin Shi Pingping Liu Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress iScience Natural sciences Plant biochemistry Plant bioinformatics Plant Biology Plant physiology Plant systematics |
| title | Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress |
| title_full | Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress |
| title_fullStr | Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress |
| title_full_unstemmed | Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress |
| title_short | Proteomics and metabolomics analyses of tobacco protoplasts under Cd stress |
| title_sort | proteomics and metabolomics analyses of tobacco protoplasts under cd stress |
| topic | Natural sciences Plant biochemistry Plant bioinformatics Plant Biology Plant physiology Plant systematics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225015986 |
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