Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation

Serratia marcescens is known for its high production of prodigiosin, a pigment that serves as a virulence factor and possesses beneficial biological, antibacterial, antifungal, and antimalarial characteristics. Another virulence factor of Serratia marcescens is the ability to produce biofilms, which...

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Main Authors: Lubna Abdul Kareem, Ali Al-Dewan
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2024-12-01
Series:Basrah Journal of Veterinary Research
Subjects:
Online Access:https://bjvr.uobasrah.edu.iq/article_185766_7f582118ac87b5e9adf64c85fdd8fe51.pdf
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author Lubna Abdul Kareem
Ali Al-Dewan
author_facet Lubna Abdul Kareem
Ali Al-Dewan
author_sort Lubna Abdul Kareem
collection DOAJ
description Serratia marcescens is known for its high production of prodigiosin, a pigment that serves as a virulence factor and possesses beneficial biological, antibacterial, antifungal, and antimalarial characteristics. Another virulence factor of Serratia marcescens is the ability to produce biofilms, which are aggregations of microorganisms adhering to surfaces surrounded by self-produced matrix. These biofilms pose many health risks in the milk production and processing industry, such as milk spoilage, poor-quality milk products, and other health risks. Our study revealed that, after 24 hours of incubation at 30°C, colonies on Chrome agar appeared pink with a dark center while on nutrient agar colonies appeared red at 28°C due to Serratia marcescens' ability to produce pigment. the ability of these local isolates to produce pigment was evaluated using NB Medium with different incubation time A total of 11 isolates from milk samples showed the ability to produce a high concentration of prodigiosin when incubated for 72 hours. All isolates were found to produce biofilm at different rates. In isolate (6), we observed the highest production of biofilm.
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publishDate 2024-12-01
publisher College of Veterinary Medicine, University of Basrah, Iraq
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spelling doaj-art-c003deff85cd4057bcbda15887905aef2025-08-20T03:09:58ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562024-12-0123411713010.23975/bjvr.2024.153110.1135185766Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm FormationLubna Abdul Kareem0Ali Al-DewanDepartment of Veterinary Microbiology, Veterinary Medicine College, University of Basrah, Iraq.Serratia marcescens is known for its high production of prodigiosin, a pigment that serves as a virulence factor and possesses beneficial biological, antibacterial, antifungal, and antimalarial characteristics. Another virulence factor of Serratia marcescens is the ability to produce biofilms, which are aggregations of microorganisms adhering to surfaces surrounded by self-produced matrix. These biofilms pose many health risks in the milk production and processing industry, such as milk spoilage, poor-quality milk products, and other health risks. Our study revealed that, after 24 hours of incubation at 30°C, colonies on Chrome agar appeared pink with a dark center while on nutrient agar colonies appeared red at 28°C due to Serratia marcescens' ability to produce pigment. the ability of these local isolates to produce pigment was evaluated using NB Medium with different incubation time A total of 11 isolates from milk samples showed the ability to produce a high concentration of prodigiosin when incubated for 72 hours. All isolates were found to produce biofilm at different rates. In isolate (6), we observed the highest production of biofilm.https://bjvr.uobasrah.edu.iq/article_185766_7f582118ac87b5e9adf64c85fdd8fe51.pdfserratia marcescensprodigiosinpigmentbiofilmantibacterial
spellingShingle Lubna Abdul Kareem
Ali Al-Dewan
Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
Basrah Journal of Veterinary Research
serratia marcescens
prodigiosin
pigment
biofilm
antibacterial
title Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
title_full Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
title_fullStr Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
title_full_unstemmed Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
title_short Characterization of Serratia marcescens: Prodigiosin Synthesis under Various Environmental Conditions and Biofilm Formation
title_sort characterization of serratia marcescens prodigiosin synthesis under various environmental conditions and biofilm formation
topic serratia marcescens
prodigiosin
pigment
biofilm
antibacterial
url https://bjvr.uobasrah.edu.iq/article_185766_7f582118ac87b5e9adf64c85fdd8fe51.pdf
work_keys_str_mv AT lubnaabdulkareem characterizationofserratiamarcescensprodigiosinsynthesisundervariousenvironmentalconditionsandbiofilmformation
AT alialdewan characterizationofserratiamarcescensprodigiosinsynthesisundervariousenvironmentalconditionsandbiofilmformation