Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications

This study describes a sediment sampling protocol using a Kinoshita-type grab (K-grab) sediment sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. During the GB24 geological survey cruise aboard the Bosei-maru, 133 surface sediment samples were...

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Main Authors: Mutsumi Iizuka, Atsuko Amano, Takuya Itaki
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:MethodsX
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Online Access:http://www.sciencedirect.com/science/article/pii/S2215016125000603
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author Mutsumi Iizuka
Atsuko Amano
Takuya Itaki
author_facet Mutsumi Iizuka
Atsuko Amano
Takuya Itaki
author_sort Mutsumi Iizuka
collection DOAJ
description This study describes a sediment sampling protocol using a Kinoshita-type grab (K-grab) sediment sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. During the GB24 geological survey cruise aboard the Bosei-maru, 133 surface sediment samples were collected from depths of 20–800 m. The K-grab, equipped with a head-slide weight mechanism, enhanced sampling efficiency across various sediment types. For microplastics, stainless steel containers and J-shaped aluminum tubes minimized contamination while maintaining sample integrity. Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination. These results demonstrate that the K-grab is a reliable tool for microplastic and macroplastic analysis, providing valuable data on plastic pollution in marine sediments. • This study describes a sediment sampling protocol using a grab sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. • During the survey, 133 surface sediment samples were collected from depths of 20–800 m, with microplastics handled using J-shaped aluminum tubes and stainless steel containers to minimize contamination while maintaining sample integrity. • Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination.
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publishDate 2025-06-01
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spelling doaj-art-bffd4a6aa3ae4e9bbf60cc9b803fd2622025-08-20T02:35:30ZengElsevierMethodsX2215-01612025-06-011410321310.1016/j.mex.2025.103213Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applicationsMutsumi Iizuka0Atsuko Amano1Takuya Itaki2The Research Institute of Geology and Geoinformation, Geological Survey of Japan, AIST, Tsukuba Central 7 AIST, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan; Corresponding author.The Research Institute of Geology and Geoinformation, Geological Survey of Japan, AIST, Tsukuba Central 7 AIST, 1-1-1 Higashi, Tsukuba, Ibaraki, JapanThe Research Institute of Geology and Geoinformation, Geological Survey of Japan, AIST, Tsukuba Central 7 AIST, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan; Estuary Research Center, Shimane University, 1060 Nishikawatu-cho, Matsue, Shimane, JapanThis study describes a sediment sampling protocol using a Kinoshita-type grab (K-grab) sediment sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. During the GB24 geological survey cruise aboard the Bosei-maru, 133 surface sediment samples were collected from depths of 20–800 m. The K-grab, equipped with a head-slide weight mechanism, enhanced sampling efficiency across various sediment types. For microplastics, stainless steel containers and J-shaped aluminum tubes minimized contamination while maintaining sample integrity. Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination. These results demonstrate that the K-grab is a reliable tool for microplastic and macroplastic analysis, providing valuable data on plastic pollution in marine sediments. • This study describes a sediment sampling protocol using a grab sampler to collect and analyze microplastics (<5 mm) and macroplastics (>5 mm) in marine sediments. • During the survey, 133 surface sediment samples were collected from depths of 20–800 m, with microplastics handled using J-shaped aluminum tubes and stainless steel containers to minimize contamination while maintaining sample integrity. • Macroplastics were separated using a 5 mm mesh and analyzed on board. Method verification confirmed high-spatial-resolution sampling with minimal contamination.http://www.sciencedirect.com/science/article/pii/S2215016125000603A sediment sampling protocol for analyzing microplastics and macroplastics using a K-grab
spellingShingle Mutsumi Iizuka
Atsuko Amano
Takuya Itaki
Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
MethodsX
A sediment sampling protocol for analyzing microplastics and macroplastics using a K-grab
title Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
title_full Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
title_fullStr Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
title_full_unstemmed Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
title_short Accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless-steel containers for shallow and deep-sea applications
title_sort accurate sampling of undisturbed top sediment from grab sampler collected using aluminum tube and stainless steel containers for shallow and deep sea applications
topic A sediment sampling protocol for analyzing microplastics and macroplastics using a K-grab
url http://www.sciencedirect.com/science/article/pii/S2215016125000603
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AT atsukoamano accuratesamplingofundisturbedtopsedimentfromgrabsamplercollectedusingaluminumtubeandstainlesssteelcontainersforshallowanddeepseaapplications
AT takuyaitaki accuratesamplingofundisturbedtopsedimentfromgrabsamplercollectedusingaluminumtubeandstainlesssteelcontainersforshallowanddeepseaapplications