Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant
Tetragonolobus palaestinus Bioss (Aljalaton) is one of the Jordanian medicinal plants that can be used to treat stomach pain and some infections. This study was done in order to establish optimal in vitro propagation method for T. palaestinus. Factors of in vitro shooting, rooting, and acclimatizati...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2022-10-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/13107 |
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| author | Mawadda MHERAT Mohammad SHATNAWI Rida SHIBLI Tamara QUDAH Saida ABU MALLOH Tamadour AL-QUDAH |
| author_facet | Mawadda MHERAT Mohammad SHATNAWI Rida SHIBLI Tamara QUDAH Saida ABU MALLOH Tamadour AL-QUDAH |
| author_sort | Mawadda MHERAT |
| collection | DOAJ |
| description | Tetragonolobus palaestinus Bioss (Aljalaton) is one of the Jordanian medicinal plants that can be used to treat stomach pain and some infections. This study was done in order to establish optimal in vitro propagation method for T. palaestinus. Factors of in vitro shooting, rooting, and acclimatization of the in vitro Tetragonolobus palaestinus seedlings were studied using different growth regulators. For in vitro shooting, different cytokinins including benzylamino purine (BAP), kinetin, TDZ, and zeatin were used in increasing concentrations (0.0, 0.3, 0.6, 0.9, 1.2, 1.5, and 2.0 mg l-1). Using benzylamino purine (BAP produced a maximum of 2.0 shoots/explants on Murashige and Skoog (MS) medium supplemented with 0.3 mg l-1. Moreover, the effect of different concentrations of IBA (indole-3-butyric acid), IAA (indole-3-acetic acid), andnaphthalene acetic acid (NAA) was evaluated for in vitro rooting. The highest number of roots (4.06 roots/explant) was obtained on MS medium supplemented with 0.3 mg l-1 IBA. All of the plants (100 %) were grown normally after the acclimatization process. Based on these results simple protocol of T. palaestinus in vitro culture was optimized for the first time which can be utilized to do more studies on cell culture and production of active secondary metabolites. |
| format | Article |
| id | doaj-art-bfec1e7481df41ee8638fb49323cacaa |
| institution | OA Journals |
| issn | 1854-1941 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-bfec1e7481df41ee8638fb49323cacaa2025-08-20T02:05:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412022-10-0111831910.14720/aas.2022.118.3.120819499Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plantMawadda MHERAT0Mohammad SHATNAWI1Rida SHIBLI2Tamara QUDAH3Saida ABU MALLOH4Tamadour AL-QUDAH5Faculty of Agricultural technology, Al Balqa Applied University, Salt, JordanFaculty of Agricultural technology, Al Balqa Applied University, Salt, JordanAl Ahliyya Amman University (and The University of Jordan), Faculty of Agricultural technology, Amman, JordanHamdi Mango Centre for Scientific Research, University of Jordan, JordanHamdi Mango Centre for Scientific Research, University of Jordan, JordanDepartment of Nutrition and Food Technology, Faculty of Agriculture, Mutah University, Karak, JordanTetragonolobus palaestinus Bioss (Aljalaton) is one of the Jordanian medicinal plants that can be used to treat stomach pain and some infections. This study was done in order to establish optimal in vitro propagation method for T. palaestinus. Factors of in vitro shooting, rooting, and acclimatization of the in vitro Tetragonolobus palaestinus seedlings were studied using different growth regulators. For in vitro shooting, different cytokinins including benzylamino purine (BAP), kinetin, TDZ, and zeatin were used in increasing concentrations (0.0, 0.3, 0.6, 0.9, 1.2, 1.5, and 2.0 mg l-1). Using benzylamino purine (BAP produced a maximum of 2.0 shoots/explants on Murashige and Skoog (MS) medium supplemented with 0.3 mg l-1. Moreover, the effect of different concentrations of IBA (indole-3-butyric acid), IAA (indole-3-acetic acid), andnaphthalene acetic acid (NAA) was evaluated for in vitro rooting. The highest number of roots (4.06 roots/explant) was obtained on MS medium supplemented with 0.3 mg l-1 IBA. All of the plants (100 %) were grown normally after the acclimatization process. Based on these results simple protocol of T. palaestinus in vitro culture was optimized for the first time which can be utilized to do more studies on cell culture and production of active secondary metabolites.https://journals.uni-lj.si/aas/article/view/13107acclimatizationin vitroshoot multiplicationrooting |
| spellingShingle | Mawadda MHERAT Mohammad SHATNAWI Rida SHIBLI Tamara QUDAH Saida ABU MALLOH Tamadour AL-QUDAH Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant Acta Agriculturae Slovenica acclimatization in vitro shoot multiplication rooting |
| title | Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant |
| title_full | Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant |
| title_fullStr | Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant |
| title_full_unstemmed | Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant |
| title_short | Clonal propagation of Tetragonolobus palaestinus Bioss: A Jordanian medical plant |
| title_sort | clonal propagation of tetragonolobus palaestinus bioss a jordanian medical plant |
| topic | acclimatization in vitro shoot multiplication rooting |
| url | https://journals.uni-lj.si/aas/article/view/13107 |
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