Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1

Abstract Background A close relationship exists between castration-resistant prostate cancer (CRPC) and histidine metabolism by gut microbes. However, the effects of the histidine metabolite imidazole propionate (IMP) on prostate cancer (PCa) and its underlying mechanisms are not well understood. Me...

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Main Authors: Shengkai Jin, Yuhua Zhou, Jing Lv, Yichen Lu, Yuwei Zhang, Menglu Li, Ninghan Feng
Format: Article
Language:English
Published: BMC 2025-01-01
Series:Molecular Medicine
Subjects:
Online Access:https://doi.org/10.1186/s10020-025-01073-0
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author Shengkai Jin
Yuhua Zhou
Jing Lv
Yichen Lu
Yuwei Zhang
Menglu Li
Ninghan Feng
author_facet Shengkai Jin
Yuhua Zhou
Jing Lv
Yichen Lu
Yuwei Zhang
Menglu Li
Ninghan Feng
author_sort Shengkai Jin
collection DOAJ
description Abstract Background A close relationship exists between castration-resistant prostate cancer (CRPC) and histidine metabolism by gut microbes. However, the effects of the histidine metabolite imidazole propionate (IMP) on prostate cancer (PCa) and its underlying mechanisms are not well understood. Methods We first assessed the effects of IMP on cell proliferation and migration at the cellular level. Subsequently, we investigated the mechanism of action of IMP using transcriptome sequencing, qPCR, and Western blot analysis. Finally, we validated our findings in vivo using a mouse model. Results Histidine had no effect on PCa cell proliferation; however, IMP significantly inhibited the proliferation and migration of PC3 and DU145 cells. Mechanistic studies indicate that IMP exerts its effects by upregulating PDZK1 expression, which subsequently inhibits the phosphorylation of the PI3K-AKT pathway. Conclusions In conclusion, IMP significantly inhibits the progression of PCa, offering new insights into potential treatments for CRPC.
format Article
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institution Kabale University
issn 1528-3658
language English
publishDate 2025-01-01
publisher BMC
record_format Article
series Molecular Medicine
spelling doaj-art-bfe83bc47316435890fb62089924f9092025-01-19T12:27:22ZengBMCMolecular Medicine1528-36582025-01-0131111410.1186/s10020-025-01073-0Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1Shengkai Jin0Yuhua Zhou1Jing Lv2Yichen Lu3Yuwei Zhang4Menglu Li5Ninghan Feng6Wuxi School of Medicine, Jiangnan UniversityWuxi School of Medicine, Jiangnan UniversityWuxi School of Medicine, Jiangnan UniversityNanjing Medical UniversityNantong University Medical SchoolDepartment of Urology, Jiangnan University Medical School, Jiangnan University Medical Center (Wuxi No. 2 People’s Hospital)Wuxi School of Medicine, Jiangnan UniversityAbstract Background A close relationship exists between castration-resistant prostate cancer (CRPC) and histidine metabolism by gut microbes. However, the effects of the histidine metabolite imidazole propionate (IMP) on prostate cancer (PCa) and its underlying mechanisms are not well understood. Methods We first assessed the effects of IMP on cell proliferation and migration at the cellular level. Subsequently, we investigated the mechanism of action of IMP using transcriptome sequencing, qPCR, and Western blot analysis. Finally, we validated our findings in vivo using a mouse model. Results Histidine had no effect on PCa cell proliferation; however, IMP significantly inhibited the proliferation and migration of PC3 and DU145 cells. Mechanistic studies indicate that IMP exerts its effects by upregulating PDZK1 expression, which subsequently inhibits the phosphorylation of the PI3K-AKT pathway. Conclusions In conclusion, IMP significantly inhibits the progression of PCa, offering new insights into potential treatments for CRPC.https://doi.org/10.1186/s10020-025-01073-0Imidazole propionateHistidineProstate cancerMicrobiomeCastration-resistant prostate cancer
spellingShingle Shengkai Jin
Yuhua Zhou
Jing Lv
Yichen Lu
Yuwei Zhang
Menglu Li
Ninghan Feng
Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
Molecular Medicine
Imidazole propionate
Histidine
Prostate cancer
Microbiome
Castration-resistant prostate cancer
title Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
title_full Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
title_fullStr Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
title_full_unstemmed Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
title_short Microbially produced imidazole propionate impairs prostate cancer progression through PDZK1
title_sort microbially produced imidazole propionate impairs prostate cancer progression through pdzk1
topic Imidazole propionate
Histidine
Prostate cancer
Microbiome
Castration-resistant prostate cancer
url https://doi.org/10.1186/s10020-025-01073-0
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