Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing
ABSTRACT Background Determining the complete genome sequence data of adenoviruses has recently become greatly important due to their use by scientists as vectors in cancer studies and other fields, including vaccine development. However, the GenBank database currently has few complete genome sequenc...
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Wiley
2025-01-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70163 |
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| author | Secil Sevinc Temizkan Mehmet Cevat Temizkan |
| author_facet | Secil Sevinc Temizkan Mehmet Cevat Temizkan |
| author_sort | Secil Sevinc Temizkan |
| collection | DOAJ |
| description | ABSTRACT Background Determining the complete genome sequence data of adenoviruses has recently become greatly important due to their use by scientists as vectors in cancer studies and other fields, including vaccine development. However, the GenBank database currently has few complete genome sequences of adenoviruses, which are known for their large genomes. To address this gap, we analysed next‐generation sequencing data obtained from our previous study to provide the complete genome sequence of the canine adenovirus‐2 strain. Methods For the obtained canine adenovirus‐2 strain (OQ596341), comparative genomics, recombination and phylogenetic analysis were conducted. This sequence was compared and phylogenetically analysed with the 20 complete genome sequences of canine adenovirus previously reported in GenBank worldwide, as well as partial E3 ORFA sequences obtained from Türkiye. Results The nucleotide similarity rates of the sequence obtained from this study with other CAdV‐2 whole genomes are over 99.04%. The gene alignment results reveal that the OQ596341 was found to be closely related to the AC000020 reference genome and LC557011. There are two recombination events related to the genome in this study. Comparisons with other complete genome sequences revealed several previously unseen mutations. These mutations include H34Y in the E1A gene; P55A in the E1B 55K gene; D13N and D202N in the IVa2 gene; K679R, V934I and K989N in the Pol gene; E205K in the pTP gene; T455A in the pIIIa gene; A310V in the V gene; G151R in the protease gene; E268K in the 100K gene; G66S and G141S in the 33K gene; T14A, E250K, D287N and I293T in the E3 ORFA gene; and L193F in the E434K gene. Moreover, a comparison with partial sequences obtained from Türkiye revealed the E250K mutation in the E3 ORFA gene, which we report for the first time in Türkiye. Conclusions The complete CAdV‐2 genome sequence obtained in the present study is the first sequence from Europe. Comparative analysis with other genomes revealed some unique mutations. This study is the first to report the E250K amino acid change in the E3 ORFA gene in Türkiye. We anticipate that this data can be used in future CAdV‐2 vaccine development studies. Further studies are recommended to evaluate the impact of these mutations on viral tropism and other host interactions. |
| format | Article |
| id | doaj-art-bfe7ebb87b7149e98c5a16da928b35f2 |
| institution | DOAJ |
| issn | 2053-1095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Veterinary Medicine and Science |
| spelling | doaj-art-bfe7ebb87b7149e98c5a16da928b35f22025-08-20T02:40:41ZengWileyVeterinary Medicine and Science2053-10952025-01-01111n/an/a10.1002/vms3.70163Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation SequencingSecil Sevinc Temizkan0Mehmet Cevat Temizkan1Department of Virology Faculty of Veterinary Medicine Yozgat Bozok University Yozgat TürkiyeDepartment of Genetics Faculty of Veterinary Medicine Yozgat Bozok University Yozgat TürkiyeABSTRACT Background Determining the complete genome sequence data of adenoviruses has recently become greatly important due to their use by scientists as vectors in cancer studies and other fields, including vaccine development. However, the GenBank database currently has few complete genome sequences of adenoviruses, which are known for their large genomes. To address this gap, we analysed next‐generation sequencing data obtained from our previous study to provide the complete genome sequence of the canine adenovirus‐2 strain. Methods For the obtained canine adenovirus‐2 strain (OQ596341), comparative genomics, recombination and phylogenetic analysis were conducted. This sequence was compared and phylogenetically analysed with the 20 complete genome sequences of canine adenovirus previously reported in GenBank worldwide, as well as partial E3 ORFA sequences obtained from Türkiye. Results The nucleotide similarity rates of the sequence obtained from this study with other CAdV‐2 whole genomes are over 99.04%. The gene alignment results reveal that the OQ596341 was found to be closely related to the AC000020 reference genome and LC557011. There are two recombination events related to the genome in this study. Comparisons with other complete genome sequences revealed several previously unseen mutations. These mutations include H34Y in the E1A gene; P55A in the E1B 55K gene; D13N and D202N in the IVa2 gene; K679R, V934I and K989N in the Pol gene; E205K in the pTP gene; T455A in the pIIIa gene; A310V in the V gene; G151R in the protease gene; E268K in the 100K gene; G66S and G141S in the 33K gene; T14A, E250K, D287N and I293T in the E3 ORFA gene; and L193F in the E434K gene. Moreover, a comparison with partial sequences obtained from Türkiye revealed the E250K mutation in the E3 ORFA gene, which we report for the first time in Türkiye. Conclusions The complete CAdV‐2 genome sequence obtained in the present study is the first sequence from Europe. Comparative analysis with other genomes revealed some unique mutations. This study is the first to report the E250K amino acid change in the E3 ORFA gene in Türkiye. We anticipate that this data can be used in future CAdV‐2 vaccine development studies. Further studies are recommended to evaluate the impact of these mutations on viral tropism and other host interactions.https://doi.org/10.1002/vms3.70163canine adenovirusdoggenomenext‐generation sequencingTürkiye |
| spellingShingle | Secil Sevinc Temizkan Mehmet Cevat Temizkan Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing Veterinary Medicine and Science canine adenovirus dog genome next‐generation sequencing Türkiye |
| title | Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing |
| title_full | Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing |
| title_fullStr | Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing |
| title_full_unstemmed | Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing |
| title_short | Complete Genome Characterization of Canine Adenovirus From Türkiye With Next‐Generation Sequencing |
| title_sort | complete genome characterization of canine adenovirus from turkiye with next generation sequencing |
| topic | canine adenovirus dog genome next‐generation sequencing Türkiye |
| url | https://doi.org/10.1002/vms3.70163 |
| work_keys_str_mv | AT secilsevinctemizkan completegenomecharacterizationofcanineadenovirusfromturkiyewithnextgenerationsequencing AT mehmetcevattemizkan completegenomecharacterizationofcanineadenovirusfromturkiyewithnextgenerationsequencing |