Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis)
In order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2008-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.006 |
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| author | LI Xiao-liang LIU Gang ZHANG Yan-ming SHUAI Jiang-bing FANG Wei-huan |
| author_facet | LI Xiao-liang LIU Gang ZHANG Yan-ming SHUAI Jiang-bing FANG Wei-huan |
| author_sort | LI Xiao-liang |
| collection | DOAJ |
| description | In order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide], the lymphocyte at a density of 2×10<sup>6</sup> cells·mL<sup>-1</sup> incubated with 10 μg·mL<sup>-1</sup> concanavalin A (Con A) for 120 hours or 5 μg·mL<sup>-1</sup> phytohaemagglutinin (PHA) for 144 hours was finally determined as the optimum conditions for proliferation. The results above indicate that MTT might be a reliable method for in vitro proliferation of splenic lymphocyte in Chinese soft-shelled turtle. |
| format | Article |
| id | doaj-art-bfe299b7514b45788ee0a529068d16a6 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-bfe299b7514b45788ee0a529068d16a62025-08-20T02:54:53ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-07-013438538810.3785/j.issn.1008-9209.2008.04.00610089209Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis)LI Xiao-liangLIU GangZHANG Yan-mingSHUAI Jiang-bingFANG Wei-huanIn order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide], the lymphocyte at a density of 2×10<sup>6</sup> cells·mL<sup>-1</sup> incubated with 10 μg·mL<sup>-1</sup> concanavalin A (Con A) for 120 hours or 5 μg·mL<sup>-1</sup> phytohaemagglutinin (PHA) for 144 hours was finally determined as the optimum conditions for proliferation. The results above indicate that MTT might be a reliable method for in vitro proliferation of splenic lymphocyte in Chinese soft-shelled turtle.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.006Chinese soft-shelled turtlesplenic lymphocyteproliferationmitogenMTT |
| spellingShingle | LI Xiao-liang LIU Gang ZHANG Yan-ming SHUAI Jiang-bing FANG Wei-huan Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) 浙江大学学报. 农业与生命科学版 Chinese soft-shelled turtle splenic lymphocyte proliferation mitogen MTT |
| title | Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) |
| title_full | Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) |
| title_fullStr | Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) |
| title_full_unstemmed | Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) |
| title_short | Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis) |
| title_sort | optimum conditions of in vitro proliferation of splenic lymphocyte from chinese soft shelled turtle trionyx sinensis |
| topic | Chinese soft-shelled turtle splenic lymphocyte proliferation mitogen MTT |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.04.006 |
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