Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it
Protists are paramount for biogeochemical cycling in every aquatic ecosystem due to their vast population sizes and physiological versatility. Numerical response (NR) and functional response (FR) experiments are cornerstones of trait-based functional ecology and are increasingly studied experimental...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1559802/full |
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| author | Thomas Weisse |
| author_facet | Thomas Weisse |
| author_sort | Thomas Weisse |
| collection | DOAJ |
| description | Protists are paramount for biogeochemical cycling in every aquatic ecosystem due to their vast population sizes and physiological versatility. Numerical response (NR) and functional response (FR) experiments are cornerstones of trait-based functional ecology and are increasingly studied experimentally with phagotrophic aquatic protists. Such experiments provide estimates of protist growth, production and consumption rates in relation to biotic (food supply) and abiotic variables (e.g., temperature, pH, and salinity) that can be used in mathematical models of ecosystem dynamics. Until now, NR and FR experiments lack standardization and are subject to potential pitfalls that received little attention in the literature. It is a common misconception that an experimental investigation of a phagotrophic protist’s growth and ingestion rates represents a single experiment with replication. I demonstrate that a typical NR or FR experiment consists of a series of individual experiments in which not only the experimental target variable (food, i.e., prey abundance or biomass) changes but also other factors (physiological conditions of prey and predator, nutrient levels, unwanted contaminants) vary that may affect the experimental outcome. Standardizing all variables affecting a series of NR and FR experiments is virtually impossible. I further explain why FR experiments are more prone to experimental bias than NR experiments. Since it is principally impossible to perform an “ideal” NR or FR experiment, fulfilling all criteria of experimental standardization, the goal is to reduce the “noise” to obtain statistically significant and reproducible results. To this end, I provide guidelines that may help achieve this goal in future studies. |
| format | Article |
| id | doaj-art-bfd93186e43b4baa8d817b65e1494caf |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-bfd93186e43b4baa8d817b65e1494caf2025-08-20T03:44:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15598021559802Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get itThomas WeisseProtists are paramount for biogeochemical cycling in every aquatic ecosystem due to their vast population sizes and physiological versatility. Numerical response (NR) and functional response (FR) experiments are cornerstones of trait-based functional ecology and are increasingly studied experimentally with phagotrophic aquatic protists. Such experiments provide estimates of protist growth, production and consumption rates in relation to biotic (food supply) and abiotic variables (e.g., temperature, pH, and salinity) that can be used in mathematical models of ecosystem dynamics. Until now, NR and FR experiments lack standardization and are subject to potential pitfalls that received little attention in the literature. It is a common misconception that an experimental investigation of a phagotrophic protist’s growth and ingestion rates represents a single experiment with replication. I demonstrate that a typical NR or FR experiment consists of a series of individual experiments in which not only the experimental target variable (food, i.e., prey abundance or biomass) changes but also other factors (physiological conditions of prey and predator, nutrient levels, unwanted contaminants) vary that may affect the experimental outcome. Standardizing all variables affecting a series of NR and FR experiments is virtually impossible. I further explain why FR experiments are more prone to experimental bias than NR experiments. Since it is principally impossible to perform an “ideal” NR or FR experiment, fulfilling all criteria of experimental standardization, the goal is to reduce the “noise” to obtain statistically significant and reproducible results. To this end, I provide guidelines that may help achieve this goal in future studies.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1559802/fullfunctional ecologyexperimental designnoise and biasgrowth ratesingestion ratesciliates |
| spellingShingle | Thomas Weisse Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it Frontiers in Microbiology functional ecology experimental design noise and bias growth rates ingestion rates ciliates |
| title | Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it |
| title_full | Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it |
| title_fullStr | Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it |
| title_full_unstemmed | Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it |
| title_short | Numerical and functional response of phagotrophic aquatic protists: the ideal experiment—and why we cannot get it |
| title_sort | numerical and functional response of phagotrophic aquatic protists the ideal experiment and why we cannot get it |
| topic | functional ecology experimental design noise and bias growth rates ingestion rates ciliates |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1559802/full |
| work_keys_str_mv | AT thomasweisse numericalandfunctionalresponseofphagotrophicaquaticprotiststheidealexperimentandwhywecannotgetit |