Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers

Microemulsions are relatively stable carrier agents for bioactive components. This study aimed to determine the characteristics of oil-in-water (O/W) microemulsions as carriers of α-tocopherol compounds in variations of α-tocopherol concentration, pH, and dilution. The O/W microemulsion was made fro...

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Main Authors: I Dewa Gde Mayun Permana, Lutfi Suhendra, Bambang Admadi Harsojuwono, Ida Bagus Wayan Gunam
Format: Article
Language:English
Published: National Centre of Excellence in Analytical Chemsitry 2025-06-01
Series:Pakistan Journal of Analytical & Environmental Chemistry
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Online Access:https://pjaec.pk/index.php/pjaec/article/view/12
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author I Dewa Gde Mayun Permana
Lutfi Suhendra
Bambang Admadi Harsojuwono
Ida Bagus Wayan Gunam
author_facet I Dewa Gde Mayun Permana
Lutfi Suhendra
Bambang Admadi Harsojuwono
Ida Bagus Wayan Gunam
author_sort I Dewa Gde Mayun Permana
collection DOAJ
description Microemulsions are relatively stable carrier agents for bioactive components. This study aimed to determine the characteristics of oil-in-water (O/W) microemulsions as carriers of α-tocopherol compounds in variations of α-tocopherol concentration, pH, and dilution. The O/W microemulsion was made from a mixture of surfactants (Tween 80, Tween 20, and Span 80) with virgin coconut oil (VCO) in a ratio of 85:15 with a total volume of 5 mL added with 10 mL of distilled water. The research used a randomized block design (RBD). The data were analyzed using ANOVA and continued with Tukey's test and regression analysis. Treatments in microemulsion were the addition of α-tocopherol with concentrations of: 0.25, 1.50, 1.75 and 2.0%. Observations were made on the turbidity index values, appearance, droplet diameter, and microemulsion stability. In addition, observations were made on the stability of the microemulsion at variations of pH (4.5,5.5, 6.5), and dilution (10, 50, and 100 times). The results showed that the microemulsion was able to carry α-tocopherol compounds up to a concentration of 2% with a turbidity value reaching 0.1036 ± 0.0033% with a transparent appearance. A mixture of three surfactants (Tween 80, Tween 20 and Span 80) has the ability to increase droplet stability. The α-tocopherol microemulsion had a droplet diameter below 30 nm and stayed stable against the effects of centrifugation at pH 4.5 with dilutions up to 50 times and remained stable upto pH 6.5 with dilutions between 50 – 100 times.
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spelling doaj-art-bfd8b9df4f544fd89dec6ef09ca8dde82025-08-20T03:17:56ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552025-06-01250112613410.21743/pjaec/2024.06.12Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol CarriersI Dewa Gde Mayun Permana 0Lutfi Suhendra 1Bambang Admadi Harsojuwono2Ida Bagus Wayan Gunam3Department of Food Science and Technology, Agriculture Technology Faculty, Udayana University, Badung (80361), Bali, IndonesiaDepartment of Agroindustry Technology, Agriculture Technology Faculty, Udayana University, Badung (80361), Bali, IndonesiaDepartment of Agroindustry Technology, Agriculture Technology Faculty, Udayana University, Badung (80361), Bali, IndonesiaDepartmentof Agroindustry Technology, Agriculture Technology Faculty, Udayana University, Badung (80361), Bali, Indonesia Microemulsions are relatively stable carrier agents for bioactive components. This study aimed to determine the characteristics of oil-in-water (O/W) microemulsions as carriers of α-tocopherol compounds in variations of α-tocopherol concentration, pH, and dilution. The O/W microemulsion was made from a mixture of surfactants (Tween 80, Tween 20, and Span 80) with virgin coconut oil (VCO) in a ratio of 85:15 with a total volume of 5 mL added with 10 mL of distilled water. The research used a randomized block design (RBD). The data were analyzed using ANOVA and continued with Tukey's test and regression analysis. Treatments in microemulsion were the addition of α-tocopherol with concentrations of: 0.25, 1.50, 1.75 and 2.0%. Observations were made on the turbidity index values, appearance, droplet diameter, and microemulsion stability. In addition, observations were made on the stability of the microemulsion at variations of pH (4.5,5.5, 6.5), and dilution (10, 50, and 100 times). The results showed that the microemulsion was able to carry α-tocopherol compounds up to a concentration of 2% with a turbidity value reaching 0.1036 ± 0.0033% with a transparent appearance. A mixture of three surfactants (Tween 80, Tween 20 and Span 80) has the ability to increase droplet stability. The α-tocopherol microemulsion had a droplet diameter below 30 nm and stayed stable against the effects of centrifugation at pH 4.5 with dilutions up to 50 times and remained stable upto pH 6.5 with dilutions between 50 – 100 times.https://pjaec.pk/index.php/pjaec/article/view/12microemulsion characteristicsconcentration of α-tocopherolsurfactant
spellingShingle I Dewa Gde Mayun Permana
Lutfi Suhendra
Bambang Admadi Harsojuwono
Ida Bagus Wayan Gunam
Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
Pakistan Journal of Analytical & Environmental Chemistry
microemulsion characteristics
concentration of α-tocopherol
surfactant
title Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
title_full Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
title_fullStr Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
title_full_unstemmed Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
title_short Microemulsion Characteristics of Oil-in-Water with Surfactants as α-tocopherol Carriers
title_sort microemulsion characteristics of oil in water with surfactants as α tocopherol carriers
topic microemulsion characteristics
concentration of α-tocopherol
surfactant
url https://pjaec.pk/index.php/pjaec/article/view/12
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