In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses

Bacteriocin(s) producing lactic acid bacteria naturally present in traditional cheeses represent an inexhaustive pool of microbes with safeguarding potential. Some bacteriocins produced by cheese lactic acid bacteria were already described as successful anti-staphylococcal agents. The presence of ge...

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Main Authors: Aljoša Trmčić, Tanja Obermajer, Andreja Čanžek Majhenič, Irena Rogelj, Bojana Bogovič Matijašić
Format: Article
Language:English
Published: Croatian Dairy Union 2010-09-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=89058
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author Aljoša Trmčić
Tanja Obermajer
Andreja Čanžek Majhenič
Irena Rogelj
Bojana Bogovič Matijašić
author_facet Aljoša Trmčić
Tanja Obermajer
Andreja Čanžek Majhenič
Irena Rogelj
Bojana Bogovič Matijašić
author_sort Aljoša Trmčić
collection DOAJ
description Bacteriocin(s) producing lactic acid bacteria naturally present in traditional cheeses represent an inexhaustive pool of microbes with safeguarding potential. Some bacteriocins produced by cheese lactic acid bacteria were already described as successful anti-staphylococcal agents. The presence of genes for bacteriocins with potential anti-staphylococcal activity was also demonstrated in two Slovenian traditional raw milk cheeses, “Tolminc” and “Kraški ovčji sir”. Same bacteriocin genes were also detected in viable lactic acid bacteria consortia’s isolated from “bacteriocin positive cheeses” on Rogosa, M17 and CATC agar media. The aim of the research was to elucidate whether or not this particular cheese consortia, in which bacteriocin genes were detected, actually exhibit anti-staphylococcal activity in milk and/or cheese. For this purpose different cheese consortia were selected in relation to versatility of detected bacteriocin genes and used to perform challenge tests against Staphylococcus aureus in milk and cheese. In milk following the time/temperature regime of traditional cheese production all cheese consortia effectively inhibited growth of Staphylococcus aureus in the range of app. 2 to 3 log. In cheese the inhibition of staphylococci was less pronounced but still evident since inhibition of app. 1.5 log was detected. Sole inhibition by lactic acid production was ruled out whilelinking inhibition directly to bacteriocin production would take some additional work.
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publishDate 2010-09-01
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spelling doaj-art-bfbec6607516404db8b7f9d897fe18a12025-08-20T03:05:17ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252010-09-01603183190In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheesesAljoša TrmčićTanja ObermajerAndreja Čanžek MajheničIrena RogeljBojana Bogovič MatijašićBacteriocin(s) producing lactic acid bacteria naturally present in traditional cheeses represent an inexhaustive pool of microbes with safeguarding potential. Some bacteriocins produced by cheese lactic acid bacteria were already described as successful anti-staphylococcal agents. The presence of genes for bacteriocins with potential anti-staphylococcal activity was also demonstrated in two Slovenian traditional raw milk cheeses, “Tolminc” and “Kraški ovčji sir”. Same bacteriocin genes were also detected in viable lactic acid bacteria consortia’s isolated from “bacteriocin positive cheeses” on Rogosa, M17 and CATC agar media. The aim of the research was to elucidate whether or not this particular cheese consortia, in which bacteriocin genes were detected, actually exhibit anti-staphylococcal activity in milk and/or cheese. For this purpose different cheese consortia were selected in relation to versatility of detected bacteriocin genes and used to perform challenge tests against Staphylococcus aureus in milk and cheese. In milk following the time/temperature regime of traditional cheese production all cheese consortia effectively inhibited growth of Staphylococcus aureus in the range of app. 2 to 3 log. In cheese the inhibition of staphylococci was less pronounced but still evident since inhibition of app. 1.5 log was detected. Sole inhibition by lactic acid production was ruled out whilelinking inhibition directly to bacteriocin production would take some additional work.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=89058traditional cheesessafetyLAB consortiaanti-staphylococcal activity
spellingShingle Aljoša Trmčić
Tanja Obermajer
Andreja Čanžek Majhenič
Irena Rogelj
Bojana Bogovič Matijašić
In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
Mljekarstvo
traditional cheeses
safety
LAB consortia
anti-staphylococcal activity
title In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
title_full In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
title_fullStr In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
title_full_unstemmed In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
title_short In-situ inhibition of Staphylococcus aureus by lactic acid bacteria consortia from two traditional Slovenian raw milk cheeses
title_sort in situ inhibition of staphylococcus aureus by lactic acid bacteria consortia from two traditional slovenian raw milk cheeses
topic traditional cheeses
safety
LAB consortia
anti-staphylococcal activity
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=89058
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