Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens
ABSTRACT: The objective of this study was to investigate the effect of sorghum with or without protease and NSP-ase on performance, jejunal histomorphology, caecal short-chain fatty acids (SCFA), and gene expression of barrier function in the broiler chickens. A total of 240 males New Lohmann strain...
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Elsevier
2024-12-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124008770 |
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| author | Lanti Yulianti Rebecca Sitohang Ziyan A'fif Untari Zuprizal Zuprizal Muhsin Al Anas |
| author_facet | Lanti Yulianti Rebecca Sitohang Ziyan A'fif Untari Zuprizal Zuprizal Muhsin Al Anas |
| author_sort | Lanti Yulianti Rebecca Sitohang |
| collection | DOAJ |
| description | ABSTRACT: The objective of this study was to investigate the effect of sorghum with or without protease and NSP-ase on performance, jejunal histomorphology, caecal short-chain fatty acids (SCFA), and gene expression of barrier function in the broiler chickens. A total of 240 males New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 12 treatments with 4 replications, 5 birds per replicate for 35 d. The diets were a factorial combination of 3 levels of sorghum (0, 10, and 20%), 2 concentrations of protease (with and without protease), and 2 concentrations of NSP-ase (with and without NSP-ase). Results showed that villus height (VH) was improved (P < 0.05) and villus to crypt ratio (VH:CD) tended (P = 0.05) to increase with inclusion of sorghum. Inclusion of sorghum upregulated (P < 0.05) expression of the gene anti-inflammatory IL-13, and downregulated (P < 0.05) gene of toll receptor (TLR) (TLR-3 and TLR-4), pro-inflammatory (IL-18, IL-6, tumor necrosis factor-alpha (TNF-α) and tight junction zona occluding-1 (ZO-1). The result further indicated a decrease (P < 0.05) in the concentration of SCFA. The birds fed diets with protease had lower (P < 0.05) expression of gene TLR-4 and TLR-3, pro-inflammatory (IL-6 and TNF-α), and anti-inflammatory IL-10. Inclusion of sorghum markedly (P < 0.05) interacted with protease, increasing the total of SCFA, acetate, and VH in the broiler. The bird offered diets with NSP-ase had higher (P < 0.05) expression genes of pro-inflammatory IL-18 and TNF-α. Inclusion of sorghum and NSP-ase significantly (P < 0.05) interacted, upregulating gene of tight junction ZO-1 and anti-inflammatory IL-13 but downregulating gene TLR-3, and pro-inflammatory IL-6, while the SCFA production was decreased. Inclusion of sorghum in the diet with or without enzymes did not affect broiler performance (P > 0.05). In conclusion, a diet with sorghum improved jejunal histomorphology without negatively affecting growth performance. Additionally, supplementation with enzymes enhanced intestinal immune responses by up-regulating anti-inflammatory, and downregulating pro-inflammatory gene expression. |
| format | Article |
| id | doaj-art-bfbc8f9e220345a4bcb2edcfbafd274e |
| institution | DOAJ |
| issn | 0032-5791 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| spelling | doaj-art-bfbc8f9e220345a4bcb2edcfbafd274e2025-08-20T02:39:29ZengElsevierPoultry Science0032-57912024-12-011031210429810.1016/j.psj.2024.104298Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickensLanti Yulianti Rebecca Sitohang0Ziyan A'fif Untari1Zuprizal Zuprizal2Muhsin Al Anas3Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, 55281, IndonesiaDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, 55281, IndonesiaDepartment of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, 55281, IndonesiaCorresponding author:; Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Universitas Gadjah Mada Yogyakarta, 55281, IndonesiaABSTRACT: The objective of this study was to investigate the effect of sorghum with or without protease and NSP-ase on performance, jejunal histomorphology, caecal short-chain fatty acids (SCFA), and gene expression of barrier function in the broiler chickens. A total of 240 males New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 12 treatments with 4 replications, 5 birds per replicate for 35 d. The diets were a factorial combination of 3 levels of sorghum (0, 10, and 20%), 2 concentrations of protease (with and without protease), and 2 concentrations of NSP-ase (with and without NSP-ase). Results showed that villus height (VH) was improved (P < 0.05) and villus to crypt ratio (VH:CD) tended (P = 0.05) to increase with inclusion of sorghum. Inclusion of sorghum upregulated (P < 0.05) expression of the gene anti-inflammatory IL-13, and downregulated (P < 0.05) gene of toll receptor (TLR) (TLR-3 and TLR-4), pro-inflammatory (IL-18, IL-6, tumor necrosis factor-alpha (TNF-α) and tight junction zona occluding-1 (ZO-1). The result further indicated a decrease (P < 0.05) in the concentration of SCFA. The birds fed diets with protease had lower (P < 0.05) expression of gene TLR-4 and TLR-3, pro-inflammatory (IL-6 and TNF-α), and anti-inflammatory IL-10. Inclusion of sorghum markedly (P < 0.05) interacted with protease, increasing the total of SCFA, acetate, and VH in the broiler. The bird offered diets with NSP-ase had higher (P < 0.05) expression genes of pro-inflammatory IL-18 and TNF-α. Inclusion of sorghum and NSP-ase significantly (P < 0.05) interacted, upregulating gene of tight junction ZO-1 and anti-inflammatory IL-13 but downregulating gene TLR-3, and pro-inflammatory IL-6, while the SCFA production was decreased. Inclusion of sorghum in the diet with or without enzymes did not affect broiler performance (P > 0.05). In conclusion, a diet with sorghum improved jejunal histomorphology without negatively affecting growth performance. Additionally, supplementation with enzymes enhanced intestinal immune responses by up-regulating anti-inflammatory, and downregulating pro-inflammatory gene expression.http://www.sciencedirect.com/science/article/pii/S0032579124008770BroilerSorghumEnzymeShort-chain fatty acidInflammatory genes |
| spellingShingle | Lanti Yulianti Rebecca Sitohang Ziyan A'fif Untari Zuprizal Zuprizal Muhsin Al Anas Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens Poultry Science Broiler Sorghum Enzyme Short-chain fatty acid Inflammatory genes |
| title | Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| title_full | Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| title_fullStr | Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| title_full_unstemmed | Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| title_short | Inclusion of sorghum (Sorghum bicolor (L). Moench) with protease and NSP-ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| title_sort | inclusion of sorghum sorghum bicolor l moench with protease and nsp ase increases jejunal histomorphology and barrier function gene expression in broiler chickens |
| topic | Broiler Sorghum Enzyme Short-chain fatty acid Inflammatory genes |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124008770 |
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