Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages
Abstract The imbalance in the proportion of M1/M2 macrophage polarization is a crucial contributor to the persistent progression of osteoarthritis (OA). This study aimed to evaluate the effects of low-dose biocompatible ionized air (BIA) on macrophage polarization and its subsequent chondroprotectiv...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-12-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-83198-6 |
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| author | Haoran Yu Chengbiao Ding Zhongyao Hu Qi Liu Xuesong Gu Junyan He Yiqun Yan Shenrui Yu Lin Gao Wendan Cheng Zhengwei Wu Juehua Jing |
| author_facet | Haoran Yu Chengbiao Ding Zhongyao Hu Qi Liu Xuesong Gu Junyan He Yiqun Yan Shenrui Yu Lin Gao Wendan Cheng Zhengwei Wu Juehua Jing |
| author_sort | Haoran Yu |
| collection | DOAJ |
| description | Abstract The imbalance in the proportion of M1/M2 macrophage polarization is a crucial contributor to the persistent progression of osteoarthritis (OA). This study aimed to evaluate the effects of low-dose biocompatible ionized air (BIA) on macrophage polarization and its subsequent chondroprotective effects, thereby validating the potential of BIA in slowing the progression of OA. In vitro experiments demonstrated that BIA modulates the polarization of M1 macrophages toward the M2 phenotype via the ROS-mediated STAT6 pathway. This shift reduces the expression of pro-inflammatory mediators while increasing the expression of anti-inflammatory mediators and pro-chondrogenic factors, leading to an improved microenvironment surrounding chondrocytes. The direct benefits of this improved microenvironment include enhanced chondrocyte viability, inhibition of apoptosis, and reduced degradation of the extracellular matrix. In vivo studies in rats showed that BIA inhibited M1 macrophage infiltration in the synovium, upregulated the proportion of M2 macrophages, alleviated cartilage degeneration, and delayed OA progression. This gas-based regulatory strategy may open new avenues for the treatment of OA. |
| format | Article |
| id | doaj-art-bfb7d8b1c2834f6b9dc5da9384771272 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-bfb7d8b1c2834f6b9dc5da93847712722025-01-05T12:25:24ZengNature PortfolioScientific Reports2045-23222024-12-0114111810.1038/s41598-024-83198-6Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophagesHaoran Yu0Chengbiao Ding1Zhongyao Hu2Qi Liu3Xuesong Gu4Junyan He5Yiqun Yan6Shenrui Yu7Lin Gao8Wendan Cheng9Zhengwei Wu10Juehua Jing11Department of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Rehabilitation Medicine, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversitySchool of Nuclear Science and Technology, University of Science and Technology of ChinaThe Second Clinical Medical College of Anhui Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversityThe Second Clinical Medical College of Anhui Medical UniversityThe First Clinical Medical College of Anhui Medical UniversityDepartment of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversitySchool of Nuclear Science and Technology, University of Science and Technology of ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Anhui Medical UniversityAbstract The imbalance in the proportion of M1/M2 macrophage polarization is a crucial contributor to the persistent progression of osteoarthritis (OA). This study aimed to evaluate the effects of low-dose biocompatible ionized air (BIA) on macrophage polarization and its subsequent chondroprotective effects, thereby validating the potential of BIA in slowing the progression of OA. In vitro experiments demonstrated that BIA modulates the polarization of M1 macrophages toward the M2 phenotype via the ROS-mediated STAT6 pathway. This shift reduces the expression of pro-inflammatory mediators while increasing the expression of anti-inflammatory mediators and pro-chondrogenic factors, leading to an improved microenvironment surrounding chondrocytes. The direct benefits of this improved microenvironment include enhanced chondrocyte viability, inhibition of apoptosis, and reduced degradation of the extracellular matrix. In vivo studies in rats showed that BIA inhibited M1 macrophage infiltration in the synovium, upregulated the proportion of M2 macrophages, alleviated cartilage degeneration, and delayed OA progression. This gas-based regulatory strategy may open new avenues for the treatment of OA.https://doi.org/10.1038/s41598-024-83198-6Biocompatible ionized airOsteoarthritisMacrophages |
| spellingShingle | Haoran Yu Chengbiao Ding Zhongyao Hu Qi Liu Xuesong Gu Junyan He Yiqun Yan Shenrui Yu Lin Gao Wendan Cheng Zhengwei Wu Juehua Jing Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages Scientific Reports Biocompatible ionized air Osteoarthritis Macrophages |
| title | Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages |
| title_full | Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages |
| title_fullStr | Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages |
| title_full_unstemmed | Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages |
| title_short | Biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from M1 to M2 macrophages |
| title_sort | biocompatible ionized air alleviates rat osteoarthritis by modulating polarization from m1 to m2 macrophages |
| topic | Biocompatible ionized air Osteoarthritis Macrophages |
| url | https://doi.org/10.1038/s41598-024-83198-6 |
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